Operation Manual

3D Microscopy
Monitor version Axiovert 100/135/135 M
28 B 40-022-4 e 09/98
3.3 Adjustment
Use cover slip as an object and focus on it
using the10x objective;
after adjustment, check the setting using the
objective of the highest magnification
Check center position of the 3D illumination
shutter in the same way as with the eyepiece
version;
remove camera cable from the MCU II or
from the camera:
see page 21
Place the required object on the stage and
focus on it
Switch beam path to camera/TV
A 2-dimensional image first appears on the
monitor;
if the image is reversed, turn the camera
around 180°
Wear polarizing glasses
The image appears in 3 dimensions on the
monitor
Place required object on the stage,
focus on it, set Köhler illumination and
adjust aperture diaphragm until optimum 3D
contrast is achieved