Operating Manual Axio Lab.A1 www.ryfag.
Carl Zeiss Copyright / Trade Marks Axio Lab.A1 Knowledge of this manual is essential for the operation of the instrument. Please familiarize yourself with the contents of this manual and pay special attention to instructions concerning safe operation of the instrument. The specifications are subject to change; the manual is not covered by an update service.
Axio Lab.A1 Contents / List of Illustrations Carl Zeiss CONTENTS Page 1 INTRODUCTION ................................................................................................... 7 1.1 1.2 1.3 Notes on instrument safety .......................................................................................... 7 Notes on ergonomics of the microscope .................................................................... 12 Notes on warranty ..........................................................
Carl Zeiss Contents / List of Illustrations Axio Lab.A1 3.2 3.2.1 3.2.2 3.2.3 3.2.4 3.3 3.4 3.5 3.5.1 3.5.2 3.5.3 3.5.4 3.5.5 3.5.6 3.5.7 Mounting optional components ................................................................................ 62 Mounting the light intensive co-observer unit ................................................................. 62 Mounting polarizer D or filter holder ..............................................................................
Axio Lab.A1 Contents / List of Illustrations Carl Zeiss LIST OF ILLUSTRATIONS Fig. 1-1 Fig. 1-2 Fig. 2-1 Fig. 2-2 Fig. 2-3 Fig. 2-4 Fig. 2-5 Fig. 2-6 Fig. 2-7 Fig. 2-8 Fig. 2-9 Fig. 2-10 Fig. 2-11 Fig. 2-12 Fig. 2-13 Fig. 2-14 Fig. 2-15 Fig. 2-16 Fig. 2-17 Fig. 2-18 Fig. 2-19 Fig. 2-20 Fig. 2-21 Fig. 2-22 Fig. 2-23 Fig. 2-24 Fig. 3-1 Fig. 3-2 Fig. 3-3 Fig. 3-4 Fig. 3-5 Fig. 3-6 Fig. 3-7 Fig. 3-8 Fig. 3-9 Fig. 3-10 Fig. 3-11 Fig. 3-12 Fig. 3-13 Fig. 3-14 Fig. 3-15 Fig. 3-16 Fig.
Carl Zeiss Fig. 3-18 Fig. 3-19 Fig. 3-20 Fig. 3-21 Fig. 3-22 Fig. 3-23 Fig. 3-24 Fig. 3-25 Fig. 3-26 Fig. 3-27 Fig. 3-28 Fig. 3-29 Fig. 3-30 Fig. 3-31 Fig. 3-32 Fig. 3-33 Fig. 3-34 Fig. 3-35 Fig. 4-1 Fig. 4-2 Fig. 4-3 Fig. 4-4 Contents / List of Illustrations Axio Lab.A1 Fig. 4-5 Fig. 4-6 Fig. 4-7 Inserting the LED lamp ......................................................................................................58 Attaching condenser ............................................................
Axio Lab.A1 INTRODUCTION Notes on instrument safety 1 INTRODUCTION 1.1 Notes on instrument safety Carl Zeiss Axio Lab.A1 microscopes have been designed, produced and tested in compliance with DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 safety requirements for electrical measuring, control and laboratory instruments. These instruments meet the requirements of EC Directive IVDD 98/79/EC (In Vitro Diagnostic); they carry the mark.
Carl Zeiss INTRODUCTION Notes on instrument safety Axio Lab.A1 Should it be determined that protective measures are no longer effective, the instrument must be taken out of service and secured against unintended use. Please contact a Zeiss service agency or the Carl Zeiss Microscopy Service to repair the instrument. Axio Lab.
Axio Lab.A1 INTRODUCTION Notes on instrument safety Carl Zeiss LED Risk Group 2 to IEC 62471, LED radiation will be emitted. Never look into the LED beam of the illuminating device – either with or without optical instruments. Failure to observe this precaution may results in eye injuries! Combustible and easily inflammable materials should not be held close to the light beam. Be sure to read the safety data sheets for the Immersol 518 N£, Immersol 518 F£ and Immersol W£.
Carl Zeiss INTRODUCTION Notes on instrument safety Axio Lab.A1 Warning labels on Axio Lab.A1 stands Warning label: Hot surface! Affixed to all stands with transmitted light illumination. Fig. 1-1 10 "RADIATION" and "LED APERTURE" warning labels on Axio Lab.
Axio Lab.A1 Fig. 1-2 04/2013 INTRODUCTION Notes on instrument safety Carl Zeiss "Hot surface below" warning label on Axio Lab.
INTRODUCTION Notes on ergonomics of the microscope Carl Zeiss 1.2 Axio Lab.A1 Notes on ergonomics of the microscope The Axio Lab.A1 light microscope was developed and designed in cooperation with occupational health physicians and the TÜV Rhineland to meet the most exacting demands with regard to ergonomics at the microscope workstation. This first light microscope worldwide is available with a special ergonomic configuration and bears TÜV certificate ID:0000025994 "Ergonomically tested".
Axio Lab.
DESCRIPTION OF THE INSTRUMENT Intended use Carl Zeiss 2 DESCRIPTION OF THE INSTRUMENT 2.1 Intended use Axio Lab.A1 Axio Lab.A1 microscopes were designed as all-purpose microscopes for biological and medical applications, as well as material analyses. Depending on the microscope stand selected, they may also be used as true transmitted or reflected light microscopes or as combined transmitted/reflected light fluorescence microscopes. Typical biomedical applications of Axio Lab.
Axio Lab.A1 DESCRIPTION OF THE INSTRUMENT Intended use Carl Zeiss The ergonomic design elements of the Axio Lab.
DESCRIPTION OF THE INSTRUMENT System overview Carl Zeiss 2.2 16 Axio Lab.
Axio Lab.
Carl Zeiss 18 DESCRIPTION OF THE INSTRUMENT System overview 430037-7144-001 Axio Lab.
Axio Lab.
Carl Zeiss 20 DESCRIPTION OF THE INSTRUMENT System overview 430037-7144-001 Axio Lab.
Axio Lab.A1 2.3 DESCRIPTION OF THE INSTRUMENT Technical data Carl Zeiss Technical data Dimensions (width x depth x height) Axio Lab.A1 basic microscope stand without tube (430037-9000-000) ................................................ approx. 219 mm x 410 mm x 3395 mm The other stand types differ slightly in depth and significantly in height, depending on the tube used. An overview of viewing heights (eyepoint heights) of the various tubes can be found in Section 2.3.1.
Carl Zeiss DESCRIPTION OF THE INSTRUMENT Technical data Axio Lab.A1 Light sources LED transmitted light Power consumption ............................................................................................................. max. 3 W Adjustment of light source ...................................................................continuous approx. 0.5 to 12 V Halogen lighting transmitted light Power consumption ..........................................................................................
Axio Lab.A1 2.3.1 DESCRIPTION OF THE INSTRUMENT Technical data Carl Zeiss Viewing height and tube angle Order No.
DESCRIPTION OF THE INSTRUMENT Technical data Carl Zeiss 2.3.2 Axio Lab.A1 Assignment of dust covers, intermediate plate and base plate Cat. No.
DESCRIPTION OF THE INSTRUMENT Technical data Axio Lab.A1 Carl Zeiss Cat. No.
DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss 2.4 Control and functional elements on microscope 2.4.1 Stand models Axio Lab.A1 Five stand models are available in the delivery program: 1. Transmitted light stand for biomedical applications in brightfield, darkfield and phase contrast 2. Transmitted light stand for biomedical applications in brightfield, darkfield, phase contrast and polarization 3.
Axio Lab.A1 Fig. 2-1 04/2013 DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss Axio Lab.
DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss 2.4.3 Axio Lab.A1 Stand for transmitted light polarization Key to Fig.
Axio Lab.A1 Fig. 2-2 04/2013 DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss Axio Lab.
DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss 2.4.4 Axio Lab.A1 Stand for transmitted light and reflected light fluorescence Key to Fig.
Axio Lab.A1 Fig. 2-3 04/2013 DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss Axio Lab.
DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss 2.4.5 Axio Lab.A1 Stand for reflected light Key to Fig.
Axio Lab.A1 Fig. 2-4 04/2013 DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss Axio Lab.
DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss 2.4.6 Axio Lab.A1 Stand for transmitted light conoscopy Key to Fig.
Axio Lab.A1 Fig. 2-5 04/2013 DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss Axio Lab.
DESCRIPTION OF THE INSTRUMENT Control and functional elements on microscope Carl Zeiss 2.4.7 Axio Lab.A1 Ergo stands with TÜV certificate "Ergonomically tested" Key to Fig. 2-6: 1 2 3 Binocular ergo tube 8-33°, 50 mm vertically adjustable Mechanical stage 75x30 ergonomic with stationary drive Stand for transmitted light and reflected light fluorescence Fig. 2-6 Axio Lab.
Axio Lab.A1 DESCRIPTION OF THE INSTRUMENT Control and functional elements on optional components 2.5 Control and functional elements on optional components 2.5.1 Tubes/photo tubes Carl Zeiss The appropriate adapters for reflex cameras, microscope cameras and video cameras may be plugged into the camera port (Fig. 2-7/1 or Fig. 2-8/1) of the binocular photo tubes. Binocular photo tube 30°/20 with fixed graduation 50:50 Fig. 2-7 Binocular photo tube 30°/20 with fixed graduation 50:50 Fig.
Carl Zeiss DESCRIPTION OF THE INSTRUMENT Control and functional elements on optional components Axio Lab.A1 Interpupillary distance and viewing height In all tubes: The interpupillary distance can be varied by adjusting the eyepiece sockets relative to one another. The viewing height can be varied by swiveling the eyepiece sockets up (Fig. 2-9/A) or down (Fig. 2-9/B). For polarization microscopy we recommend the photo tube Pol with upright crosshairs. Fig.
Axio Lab.A1 DESCRIPTION OF THE INSTRUMENT Control and functional elements on optional components Carl Zeiss Binocular comfortable ergo tube 8-33°/22 with vertical adjustment 50 mm This comfortable ergo tube is designed for a field of view of 22. The viewing angle can be continuously adjusted between 8° and 33° by swiveling the binocular section (Fig. 2-11/3) with the aid of the angle scale (Fig. 2-11/2). The viewing height can be adjusted independently of the viewing angle.
Carl Zeiss DESCRIPTION OF THE INSTRUMENT Control and functional elements on optional components Axio Lab.A1 Binocular ergo tube/ergo photo tube 20°/23 and ergo photo tube 15°/23, each with continuous vertical adjustment These ergo photo tubes are designed for field of view of 23. For use on the Axio Lab.A1 they are recommended for max. field of view of 22. The viewing angle is 20° or 15°. Ergo tubes are continuously vertically adjustable in the range 0 mm to 44 mm. Fig.
Axio Lab.A1 2.5.2 DESCRIPTION OF THE INSTRUMENT Control and functional elements on optional components Carl Zeiss Microscope stages Mechanical stage 75x30 R or L or mechanical stage 75x30 R ergonomic with stationary drive Mechanical stage (Fig. 2-14/7) for seating, positioning and securing specimens with specimen holder. Specimen holder (Fig. 2-14/2) for singlehanded operation or specimen holder for counting chambers (replaceable after loosening the two knurled screws, Fig. 2-14/1).
Carl Zeiss DESCRIPTION OF THE INSTRUMENT Control and functional elements on optional components Axio Lab.A1 Rotary stage Pol 360° with lock Rotary stage Pol (Fig. 2-17) for seating, positioning and securing specimens with specimen guide (Fig. 2-17/5) and specimen holder (Fig. 2-17/7). 360° rotation with lock using knurled screw (Fig. 2-17/6). Angle of rotation with vernier (Fig. 2-17/1) readable on angle scale. scale Specimen guide (Fig. 2-17/5) removable (after loosening clamp screw, Fig.
Axio Lab.A1 2.5.3 DESCRIPTION OF THE INSTRUMENT Control and functional elements on optional components Carl Zeiss Condensers Condenser 0.9/1.25 H, D, Ph1, Ph2, Ph3 Condenser 0.9/1.25 H (Fig. 2-19/1) with aperture diaphragm (Fig. 2-19/4) with modulator disk (Fig. 2-19/3) for: Brightfield (H) Darkfield (D) Phase contrast Ph 1, Ph 2, Ph 3 Position adjustment of modulator disk by turning knurled ring (Fig. 2-19/2). This condenser is also available without modulator disk, i.e. only brightfield. Fig.
DESCRIPTION OF THE INSTRUMENT Control and functional elements on optional components Carl Zeiss Axio Lab.A1 Overview fixture 2.5x–4x The overview fixture is for full display field illumination when using an objective with a weak magnification factor (2.5x–4x) in combination with the Abbe condenser 0.0/1.25 H (424227-9000000). It can be centered and remains swiveled into the beam path for as long as the respective objective is in use. Swivel overview fixture (Fig.
Axio Lab.A1 2.5.4 DESCRIPTION OF THE INSTRUMENT Control and functional elements on optional components Carl Zeiss Reflector turret 4x The 4x reflector turret is equipped with reflector positions P&C. The reflector position is adjusted by turning the knurled ring (Fig. 2-23/1). The marking (Fig. 2-23/3) on the knurled ring shows the reflector position in the beam path. The stickers supplied can be used to identify the reflector modules employed. The stickers can be applied to the areas provided (Fig.
START-UP Installing standard components Carl Zeiss 3 Axio Lab.A1 START-UP The Axio Lab.A1 microscope can be independently installed, converted and started up by the customer. On request, the microscope can also be installed or converted by Zeiss Service at an extra charge. Before installing and starting-up the microscope, be sure to carefully read the Notes on instrument safety (see Section 1.1).
Axio Lab.A1 START-UP Installing standard components Carl Zeiss x For shipping the power cord (Fig. 3-3/1) can be rolled up and stowed in the open cover flap (Fig. 3-3/2). Fig. 3-3 3.1.2 Stowing power cord in cover flap for shipping Mounting base plate for use of larger tubes The base plate must be mounted on Axio Lab.A1 stands in order to increase stability during operation for the majority of tubes/photo tubes/ergo tubes, or this is at least recommended.
Carl Zeiss 3.1.3 START-UP Installing standard components Axio Lab.A1 Attaching the binocular tube/photo tube All binocular tubes listed in the system overview (refer to Section 2.2) can be mounted on the microscope stand as described below. Regardless of the stand type and tube used, in some cases an intermediate plate must also be mounted (see Section 2.2). For tubes mounted without an intermediate plate, proceed as follows: x Loosen the hexagonal socket head screw (Fig.
Axio Lab.A1 3.1.4 START-UP Installing standard components Carl Zeiss Installing eyepieces or auxiliary microscope or diopter x Remove both dust caps (Fig. 3-6/1 and 5) from the binocular tube. x Remove the two eyepieces (Fig. 3-6/2) from their cases and insert them into the binocular tube as far as they will go. Before inserting Pol eyepieces with tubes without upright reticle, the orientation screw on the reverse side of the eyepieces must be unscrewed.
START-UP Installing standard components Carl Zeiss Axio Lab.A1 Inserting reversible eyecups The eyepieces have rubber protection rings to avoid scratches on the eyeglasses. These may be replaced by reversible eyecups as desired. x For this purpose remove the eyeglass protection rings (Fig. 3-7/2) from the eyepieces and mount the eyecups (Fig. 3-7/1). Sometimes the eyeglass protection rings are seated very tightly in the eyepiece groove, so you may need a blunt object (wooden stick) to prod them off. 3.
Axio Lab.A1 3.1.6 START-UP Installing standard components Carl Zeiss Fitting and removing push&click modules in the reflector turret The reflector turret 4x stand transmitted and reflected light (BioMed) and the reflected light stand (Material) is firmly installed. The modules must be inserted and removed from the front after removing the cover cap. Inserting a module: x Remove cover cap (Fig. 3-9/4) from the stand towards the front. x Insert the module (Fig.
START-UP Installing standard components Carl Zeiss 3.1.7 Axio Lab.A1 Mounting a mechanical stage Axio Lab.A1 stands are fitted with the respective mechanical stage at the factory according to customer requirements. The friction torque of the drive knobs is set at an average value at the factory. Should the stage need to be replaced or the stage settings changed, proceed as follows: 3.1.7.1 Dismantling a stage x Remove the four fastening screws (Fig. 3-10/4) on the stage carrier (Fig.
Axio Lab.A1 3.1.7.4 START-UP Installing standard components Carl Zeiss Setting friction torque of drive knobs for X/Y adjustment of the mechanical stage (1) X drive x Push drive knob for X adjustment (Fig. 3-11/4) right to the bottom. x Remove supplied adjusting pin (Fig. 3-11/5) from the drive knob for Y adjustment (Fig. 3-11/1) and insert into one of the holes of the lower hole nut(Fig. 3-11/3). x Hold the drive knob for X adjustment (Fig.
Carl Zeiss START-UP Installing standard components 3.1.8 3.1.8.1 Axio Lab.A1 Mounting rotary stage Pol Removing rotary stage pole x Loosen screw cap (Fig. 3-12/6) from the spring housing (about three rotations). x Press rotary stage Pol (Fig. 3-12/4) to the front against spring-loaded pin (Fig. 3-12/7), lift it off the stage carrier (Fig. 3-12/5) from the back and remove it upwards. x Re-tighten screw cap (Fig. 3-12/6). 3.1.8.2 Attaching rotary stage Pol x Where necessary, loosen screw cap (Fig.
Axio Lab.A1 3.1.8.4 START-UP Installing standard components Carl Zeiss Removing the stage clips and mounting the detachable specimen guide Pol x Remove the stage clips (Fig. 3-12/9) from the rotary stage Pol. x Insert the specimen guide Pol (Fig. 3-12/2) with the two cylindrical pins on the underside into the holes provided (Fig. 3-12/3) and tighten the clamp screw (Fig. 3-12/1). 3.1.8.5 Centering rotary stage Pol With high-power objectives centering can be exact only for one selected objective.
START-UP Installing standard components Carl Zeiss 3.1.8.6 Centring objectives polarization stand Axio Lab.A1 of the The nosepiece 4x Pol is equipped with one fixed and three centerable objective positions. Stage centering of the non-centering objective mount is necessary to ensure that a specimen feature located in the center of the field of view does not drift out while rotating the stage.
Axio Lab.A1 3.1.9 START-UP Installing standard components Carl Zeiss Attaching condenser x Move the stage carrier with focusing drive to the higher stop position. CAUTION The objectives should not collide with other parts. x Swivel out front lens (if shiftable) on condenser using lever (Fig. 3-15/7). x Remove both centering screws (Fig. 3-15/5) on the condenser carrier until their ends are no longer visible. x Push the condenser carrier (Fig. 3-15/3) with drive knob for vertical adjustment (Fig.
START-UP Installing standard components Carl Zeiss 3.1.10 Axio Lab.A1 Installing or replacing 35 W halogen bulb or 3 W white light LED lamp If desired, Axio Lab.A1 transmitted light stands can be equipped with a 3 W white light LED lamp with spectrum daylight or warm light. To insert or replace the halogen/LED lamp, proceed as follows: x Switch off the microscope, remove the power cord on the microscope and allow it to cool down at least 15 min. Fig.
Axio Lab.A1 START-UP Installing standard components Carl Zeiss x Check that the lighting unit is seated correctly and insert the plug (Fig. 3-19/2) onto the pins of the lighting unit (Fig. 3-19/1). Ensure that it engages properly to avoid bending the pins. x Insert the cable of the lamp plug into the stand so that it is not damaged when the cover is mounted. x Insert the lower edge of the cover (Fig. 3-16/2) into the retaining channels (Fig.
START-UP Installing standard components Carl Zeiss 3.1.11 Axio Lab.A1 Installing or changing the 12 V 50 W halogen lamp Each Axio Lab.A1 reflected light stand is equipped with a 12 V 50 W halogen lamp. To insert or replace a halogen lamp, proceed as follows: x Switch off the microscope, remove the power cord on the microscope and allow it to cool down at least 15 min. x Loosen the screw (Fig. 3-20/2) in the cover. Fig. 3-20 x Swing the cover (Fig.
Axio Lab.A1 3.1.12 START-UP Installing standard components Carl Zeiss Installing or replacing LED modules Axio Lab.A1 transmitted/reflected light stands may be equipped for transmitted light applications with a 35 W halogen lamp or an 3 W LED lamp (see Section 3.1.10) and for reflected light fluoresce applications with up to two LED modules from the delivery program (see also Section 2.2). To insert or replace LED modules, proceed as follows: x Switch off the microscope, disconnect the power cord.
Carl Zeiss 3.2 START-UP Mounting optional components Axio Lab.A1 Mounting optional components The microscope must be switched off and unplugged before starting work. Upon completion of work the respective modules must be restored to function (see Sections 3.1 to 3.4). 3.2.1 Mounting the light intensive co-observer unit The light-intensive co-observer unit is mounted on the Axio Lab.
Axio Lab.A1 3.2.3 START-UP Mounting optional components Carl Zeiss Mounting and centering the overview fixture x Lift the condenser carrier together with its drive knob upwards as far as it will go. x Remove the polarizer or filter holder from the condenser carrier as applicable. x Hold the overview fixture or filter holder (Fig. 3-27/2) parallel to the underside of the condenser carrier (Fig. 3-27/1) and screw the holding pin (Fig. 3-27/4) of overview fixture with the angled adjusting lever (Fig.
Carl Zeiss START-UP Mounting optional components 3.2.4 Inserting modulator condenser 0.9 H Pol Axio Lab.A1 disk in x Remove the condenser (Fig. 3-28/1) from the condenser carrier (see Section 3.1.9). If the condenser carrier cannot be lowered sufficiently, e.g. when the overview fixture is fitted, the latter must be detached to enable the condenser to be pushed down as far as it will go and removed. x Loosen the clamp screw (Fig. 3-28/5) of the condenser scale segment (Fig.
Axio Lab.A1 3.3 START-UP Connecting to the power supply Carl Zeiss Connecting to the power supply In all stand models the power supply of the Axio Lab.A1 is located in the rear side of the instrument. x Connect microscope (Fig. 3-29/1) to the mains power supply via a power cord and mains socket. x The Axio Lab.A1 can be connected to a line voltage of 100 to 240 VAC, 50/60 Hz. The power unit is set automatically to the line voltage available. 3.4 Fig.
Carl Zeiss START-UP Basic setting of the microscope from an ergonomic point of view 3.5 Basic setting of the microscope from an ergonomic point of view 3.5.1 Configuration of an ergonomic microscope workplace Axio Lab.A1 The Axio Lab.A1 light microscope was developed and designed in cooperation with occupational health physicians and the TÜV Rhineland to meet the most exacting demands with regard to ergonomics at the microscope workstation.
Axio Lab.A1 3.5.2 START-UP Basic setting of the microscope from an ergonomic point of view Carl Zeiss TÜV certificate ID:0000025994 "Ergonomically tested" TÜV certificate ID:0000025994 "Ergonomically tested" prescribes the distance of control elements from the table, the user and each other. Furthermore, it defines a broad adjustment range for the eyepiece to cater for the different body heights of male and female microscope users worldwide.
Carl Zeiss START-UP Basic setting of the microscope from an ergonomic point of view Axio Lab.A1 The microscope workstation must be separate from the general laboratory area to enable uninterrupted work, in particular for medium and long periods of work.
Axio Lab.A1 START-UP Basic setting of the microscope from an ergonomic point of view Carl Zeiss For example, in the TÜV-certified ergonomic configuration, including the comfortable ergo tube (4255229040-000), the eyepiece viewing height and angle are continuously variable to cover the range from the 5th percentile female to the 95th percentile male. When using other ergo tubes from the Axio Lab.A1 program the coverage of the setting range is somewhat smaller.
Carl Zeiss START-UP Basic setting of the microscope from an ergonomic point of view 3.5.5 Axio Lab.A1 Setting interpupillary distance on the binocular tube x Adjusting individual interpupillary distance by symmetrical swiveling of the two eyepiece sockets against each other (Fig. 3-34). The correct interpupillary distance has been set when the observer sees only one round image when looking into the eyepiece! Fig. 3-34 Setting the interpupillary distance on the binocular tube 3.5.
Axio Lab.A1 3.5.7 START-UP Basic setting of the microscope from an ergonomic point of view Carl Zeiss Adjusting for ametropia when using eyepiece reticles The prerequisite for correct use of an eyepiece reticle is two adjustable eyepieces to compensate for different degrees of ametropia of the user. x Focus on the line figure of the eyepiece reticle with the focusable eye lens of the adjustable eyepiece.
OPERATION Lighting and contrasting method in transmitted light Carl Zeiss 4 OPERATION 4.1 Lighting and contrasting method in transmitted light 4.1.1 Adjusting the transmitted light brightfield according to KÖHLER Axio Lab.A1 (1) General principle Transmitted light brightfield microscopy is the most common optical microscopy method, as highcontrast or colored specimens (e.g. blood smears) can be viewed quickly and easily. For a true-to-object imaging, indirect ray bundles, i.e.
Axio Lab.A1 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss x Close luminous-field diaphragm (Fig. 4-1/5) until it is visible (even if not in focus) in the field of view (Fig. 4-1/A). x Turn the vertical control of the condenser drive to lower the condenser until the edge of the luminous-field diaphragm appears in focus (Fig. 4-1/B). x Center the luminous-field diaphragm using the two centering screws (Fig. 4-1/4) on the condenser carrier (Fig.
Carl Zeiss OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 (4) Setting the height stop on the condenser carrier x Loosen the fastening screw (Fig. 4-2/1) of the height stop using a SW 3 ball-headed screwdriver. x Use focusing drive to focus on the specimen. x Close luminous-field diaphragm and focus it by turning the vertical control (Fig. 4-2/2) of the condenser. x Carefully raise the condenser slightly without lifting the specimen. Fig.
Axio Lab.A1 4.1.2 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss Adjusting the transmitted light darkfield according to KÖHLER (1) General principle Due to their transparency, unstained biological specimens, such as bacteria or living cell cultures, are often barely or not at all visible in transmitted light bright field. This is radically changed when such specimens are observed in the transmitted light darkfield.
OPERATION Lighting and contrasting method in transmitted light Carl Zeiss Axio Lab.A1 x Reinsert the eyepiece into the tube. x If the height of the darkfield condenser is set correctly and sensitively, it is possible to reduce any brightening in the field of view left, and the luminous-field diaphragm image appears almost perfectly in focus. x Finally, match the size of the luminous-field diaphragm to the size of the field of view.
Axio Lab.A1 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss (3) Setting transmitted light phase contrast x Swivel the phase contrast objective, e.g. labeled Ph 1, into the beam path. x On the turret disk of the condenser, swivel in the phase stop to match the phase contrast objective, e.g. 1.
OPERATION Lighting and contrasting method in transmitted light Carl Zeiss 4.1.4 4.1.4.1 Axio Lab.A1 Setting transmitted light polarization Detecting birefringence (1) Application The transmitted light polarization method is used for specimens which change the state of polarization of light. These specimens, such as crystals, minerals or polymers, are referred to as birefringent.
Axio Lab.A1 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss (3) Setting the microscope x Set the microscope as in the transmitted light brightfield according to KÖHLER (see Section 4.1.1 (3)). x Center rotary stage Pol (Fig. 4-5/1) (see Section 3.1.8.5) and objectives (see Section 3.1.8.6). x Swivel polarizer (Fig. 4-5/3) into the light path and, if it is rotatable, position it at 0°. x Insert the analyzer slider (Fig.
Carl Zeiss 4.1.4.2 OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 Determination of gout and pseudogout x Set the microscope as in the transmitted light brightfield according to KÖHLER (see Section 4.1.1 (3)). x Then swivel the polarizer, rigidly fixed to the lambda plate (445226-0000-000) (Fig. 4-5/3) into the beam path. x Insert the analyzer slider (Fig. 4-5/2) into the slit for compensators. x The field of view will appear dark due to the crossed polarizers.
OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 4.1.4.3 Carl Zeiss Determining the direction of oscillation nJ (1) Application The determination of the direction of oscillation of nJ and nJ' (direction of oscillation with the absolutely or relatively highest refractive index) and nD and nD' (direction of oscillation with the absolutely or relatively lowest refractive index) in relation to the morphological directions, e.g.
Carl Zeiss OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 (3) Setting the microscope x Set the microscope as for the transmitted light brightfield (see Section 4.1.1), taking care to ensure the correct interpupillary distance in the binocular tube (see Section 3.5.5). x Center rotary stage Pol (Fig. 4-5/1) and objectives (see Sections 3.1.8.5 and 3.1.8.6). x Swivel polarizer (Fig. 4-5/3) into the light path and, if it is rotatable, position it at 0°.
Axio Lab.A1 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss (4) Conclusions The grayish-white color appearing first in the bright position in the above example (Fig. 4-7/1) corresponds to a path difference of 150 nm according to the Michel-Lévy color chart (Fig. 4-8).
Carl Zeiss OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 x Limit to aperture to a value of about 0.2. x Turn the rotary stage Pol until the specimen is almost obliterated, i.e. completely dark, and set the 45° locking position. x Rotate the stage once (by 45°) so that the specimen is in a diagonal position (bright).
OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 4.1.4.5 Carl Zeiss Circular polarization contrast (1) Application Unlike standard polarization contrast, circular polarization contrast does not show any dark (extinction) positions that depend on the angle of rotation (azimuth) of the specimen relative to polarizer or analyzer. This means that when the stage is rotated the same image impression remains, as the light/dark positions are omitted.
Carl Zeiss 1 2 3 4 5 OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 Slide 6x20 with O/4 plate Lower section of circular polarizer Lever for rotating O/4 plate O/4 plate in upper part of the circular polarizer Adjustment slits Fig. 4-9 Components for circular polarization contrast x An (anisotropic) specimen should not be observed until after the above adjustment. x Reinsert the specimen to be examined.
Axio Lab.A1 OPERATION Lighting and contrasting method in transmitted light 4.1.5 Setting transmitted light polarization with the conoscopy stand 4.1.6 Determining the optical character of crystals Carl Zeiss For the classification (and thus identification) of crystalline material – instead of the observation of the specimen itself – the analysis of an interference image in the objective pupil provides the more valuable information.
Carl Zeiss OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 x Set the microscope as in the transmitted light brightfield according to KÖHLER (see Section 4.1.1). x Place the specimen on the stage and focus on it. x Swivel the analyzer into the beam path (on position) with rotary knob A (Fig. 4-10/2). The direction of oscillation can be changed using the setting wheel (Fig. 4-10/4) of the analyzer. Fig. 4-10 Axio Lab.
Axio Lab.A1 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss Inserting a compensator O (473704-0000-000) or O/4 (473714-0000-000) or a wedge compensator 0-4 O (000000-1140-663) in the compensator slot with the initial state of the axial figure being as illustrated in Fig. 4-11 results in the following changes in color shown schematically (blue and yellow areas) to the axial figure, thus allowing differentiation in "optically positive" and "optically negative".
OPERATION Lighting and contrasting method in transmitted light Carl Zeiss 4.1.6.1 Axio Lab.A1 Demonstrating birefringence with the Axio Lab for conoscopy (1) Application The transmitted light polarization method is used for specimens which change the state of polarization of light. These specimens, such as crystals, minerals or polymers, are referred to as birefringent.
Axio Lab.A1 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss (3) Setting the microscope x Set the microscope as in the transmitted light brightfield according to KÖHLER (see Section 4.1.1 (3)). x Center rotary stage Pol (Fig. 4-12/1) (see Section 3.1.8.5) and objectives (see Section 3.1.8.6). x Swivel polarizer (Fig. 4-12/3) into the light path and, if it is rotatable, position it at 0°.
OPERATION Lighting and contrasting method in transmitted light Carl Zeiss Axio Lab.A1 Evaluation: If the crystal needles parallel to the gamma direction are yellow and those perpendicular to the gamma direction are blue, they are monosodium urate crystals (gout). If the crystal needles parallel to the gamma direction are blue and those perpendicular to the gamma direction are yellow, they are calcium pyrophosphate crystals (pseudogout). 4.1.6.
Axio Lab.A1 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss (3) Setting the microscope x Set the microscope as for the transmitted light brightfield (see Section 4.1.1), taking care to ensure the correct interpupillary distance in the binocular tube (see Section 3.5.5). x Center rotary stage Pol (Fig. 4-5/1) and objectives (see Sections 3.1.8.5 and 3.1.8.6). x Swivel polarizer (Fig. 4-5/3) into the light path and, if it is rotatable, position it at 0°.
Carl Zeiss OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 (4) Conclusions The grayish-white color appearing first in the bright position in the above example (Fig. 4-14/1) corresponds to a path difference of 150 nm according to the Michel-Lévy color chart (Fig. 4-15).
Axio Lab.A1 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss x Limit to aperture to a value of about 0.2. x Turn the rotary stage Pol until the specimen is almost obliterated, i.e. completely dark. x Rotate the stage once (by 45°) so that the specimen is in a diagonal position (bright).
OPERATION Lighting and contrasting method in transmitted light Carl Zeiss 4.1.6.5 Axio Lab.A1 Circular polarization contrast with Axio Lab for conoscopy (1) Application Unlike standard polarization contrast, circular polarization contrast does not show any dark (extinction) positions that depend on the angle of rotation (azimuth) of the specimen relative to polarizer or analyzer. This means that when the stage is rotated the same image impression remains, as the light/dark positions are omitted.
Axio Lab.A1 1 2 3 4 5 OPERATION Lighting and contrasting method in transmitted light Carl Zeiss Slide 6x20 with O/4 plate Lower section of circular polarizer Lever for rotating O/4 plate O/4 plate in upper part of the circular polarizer Adjustment slits Fig. 4-16 Components for circular polarization contrast on conoscopy stand x An (anisotropic) specimen should not be observed until after the above adjustment. x Reinsert the specimen to be examined.
OPERATION Lighting and contrasting method in transmitted light Carl Zeiss 4.1.7 Axio Lab.A1 Setting transmitted light polarization for conoscopic observation – determining the optical character of crystals For the classification (and thus identification) of crystalline material – instead of the observation of the specimen itself – the analysis of an interference image in the objective pupil provides the more valuable information.
OPERATION Lighting and contrasting method in transmitted light Axio Lab.A1 Carl Zeiss x Set the microscope as in the transmitted light brightfield according to KÖHLER (see Section 4.1.1). x Swivel polarizer (Fig. 4-12/3) into the light path and, if it is rotatable, position it at 0°. x Swivel the analyzer into the beam path and bring into a crossed position with the setting wheel. (The field of view will now appear dark) x Place the specimen on the stage and focus on it.
OPERATION Lighting and contrasting method in transmitted light Carl Zeiss Axio Lab.A1 With optically biaxial crystals, the cross resolves into two dark hyperbola branches (the so-called isogyres) depending on stage rotation, which are surrounded by colored interference patterns depending on the amount of birefringence and specimen thickness (suggestive of the figure "8").
Axio Lab.A1 OPERATION Lighting and contrasting method in reflected light 4.2 Lighting and contrasting method in reflected light 4.2.1 Adjusting the reflected light brightfield according to KÖHLER Carl Zeiss (1) Application Reflected light brightfield microscopy is the simplest and most common optical microscopy method for examining opaque samples or specimens, e.g. material sections or wafers. For a true-to-object imaging, indirect ray bundles, i.e.
Carl Zeiss OPERATION Lighting and contrasting method in reflected light Axio Lab.A1 x For specimens with medium contrast characteristics, set the aperture diaphragm with the knurled wheel (Fig. 4-19/1) to about 2/3 to 4/5 of the exit pupil diameter of the objective. In most applications, this aperture diaphragm setting provides optimum contrast at almost ideal resolution, and is therefore the best compromise for the human eye.
Axio Lab.A1 4.2.2 OPERATION Lighting and contrasting method in reflected light Carl Zeiss Adjusting the reflected light darkfield (1) Application The reflected light darkfield method is used for examining incompletely reflecting surfaces with different degrees of reflectivity (ideal reflected light brightfield specimens), i.e. with scratches, ruptures, pores or other disruptions to the even surface.
OPERATION Lighting and contrasting method in reflected light Carl Zeiss 4.2.3 Axio Lab.
Axio Lab.A1 4.2.4 OPERATION Lighting and contrasting method in reflected light Carl Zeiss Adjusting reflected light fluorescence (1) General principle The reflected light fluorescence method is used to show fluorescent substances in typical fluorescence colors in high contrast. The light originating from a high-performance illuminator in a reflected light fluorescence microscope passes through a heat protection filter to an excitation filter (bandpass).
Carl Zeiss OPERATION Lighting and contrasting method in reflected light Axio Lab.A1 x On the reflector turret (Fig. 4-20/9), select reflector module FL P&C with the desired fluorescence filter combination (depending on excitation mode) and switch on. x Use the push-pull rod (Fig. 4-20/1) to swivel the desired LED (1 or 2) into the beam path. When switching between the two LEDs the current brightness setting is adopted.
Axio Lab.A1 CARE, FUSE REPLACEMENT AND SERVICE Instrument care 5 CARE, FUSE REPLACEMENT AND SERVICE 5.1 Instrument care Carl Zeiss The only care required for the Axio Lab.A1 is as follows: x Switch the device off each time after use and apply the protective cover (protects against dust and moisture). x Do not set the instrument up in a moist environment (max. humidity < 75 %). x Cover all open tubes with dust caps.
Carl Zeiss CARE, FUSE REPLACEMENT AND SERVICE Instrument maintenance 5.2 Instrument maintenance 5.2.1 Checking the instrument Axio Lab.A1 x Ensure that the prescribed line voltages are observed. x Check the power cable and the plug for possible damage. x If any damage is observed, turn the instrument off and secure immediately. Call in a qualified professional to remedy the problem. 5.2.2 Replacing the fuses in the stand Always disconnect the instrument from the power supply before replacing fuses.
Axio Lab.A1 5.3 CARE, FUSE REPLACEMENT AND SERVICE Troubleshooting Carl Zeiss Troubleshooting Problem Cause Shadows or inhomogeneous image brightness in the field of view; the field is not entirely visible. The vis/fot push-pull rod/shift knob on Move the vis/fot push-pull rod/shift the photo tube is not in the correct knob to the correct position (end position (intermediate position). position). Low resolving power and poor image contrast.
Carl Zeiss CARE, FUSE REPLACEMENT AND SERVICE Troubleshooting Axio Lab.A1 Problem Cause Troubleshooting Asymmetric image sharpness, e.g. one side is sharp, one is side blurred. Condenser is not correctly adjusted. Adjust the condenser correctly; see p. 72 ff. Nosepiece is not engaged in its locking position. Engage the nosepiece in its locking position (click-stop). The specimen has not been fixated on Insert and fixate correctly in the the mechanical stage. specimen holder.
Axio Lab.A1 CARE, FUSE REPLACEMENT AND SERVICE Troubleshooting Carl Zeiss Problem Cause Troubleshooting LED/halogen lamp does not light up although the switch in the on position. Power plug is not plugged into the mains outlet. Insert the plug into the mains outlet. Ensure that the outlet and instrument have the same voltage. Lamp is not installed. Install lamp, see p. 58. Lamp is defective. Replace lamp, see p. 58. Fuses are defective. Replace fuses, see p. 108.
CARE, FUSE REPLACEMENT AND SERVICE Service Carl Zeiss 5.4 Axio Lab.A1 Service Repairs of mechanical, optical or electronic components inside the instrument and electrical components of Axio Lab.A1 microscopes may only be performed by Carl Zeiss service staff or specially authorized personnel. To ensure optimum setting and trouble-free function of your microscope over a longer period of time, we recommend that you enter into a service/maintenance agreement with Carl Zeiss.
Axio Lab.A1 6 ANNEX 6.1 List of abbreviations AC BL Br. CSA D D d DIC DIN EC EN Ergo FL foc. fot H IEC IP ISO L LED Ph PL Pol P&C R SLR SW T TIC TL UL UV VAC vis ANNEX List of abbreviations Carl Zeiss Alternating current Bertrand lens Suitability for spectacle wearers Canadian Standards Association Frame glass thickness Darkfield Diameter (e.g.
ANNEX Index Carl Zeiss 6.2 Axio Lab.A1 Index Page A Ametropia...........................................................................................................................................71 Analyzer.......................................................................................................... 34, 78, 85, 90, 91, 93, 96 Analyzer slider .........................................................................................................................
Axio Lab.A1 ANNEX Index Carl Zeiss E Ergo photo tube ................................................................................................................................. 38 Ergo tube ..........................................................................................................................36, 38, 39, 40 Ergonomics .................................................................................................................................. 12, 36 Eyecups ...................
Carl Zeiss ANNEX Index Axio Lab.A1 Microscope adjustment .......................................................................................................................68 Microscope stages ...............................................................................................................................41 Modulator disk ....................................................................................................................................64 N Nosepiece ....................
Axio Lab.A1 ANNEX Index Carl Zeiss T Technical data .................................................................................................................................... 21 Toggle switch FL/TL............................................................................................................................. 30 Tool ................................................................................................................................................... 46 Tool flap .......
ANNEX Industrial property rights Carl Zeiss 6.3 Axio Lab.
