AxioCam MRc5 Installation + Reference - 10.2003 000000-1256-586 Number of this manual: B 48-0028 e Date of issue: 05.2003 Date of the last revision/update: 10.
Carl Zeiss Vision draws the User's attention to the fact that the information and references contained in these documents may be subject to technical modifications, in particular due to the continuous further development of Carl Zeiss Vision's products. The documents enclosed do not contain any warranty by Carl Zeiss Vision with regard to the technical processes described in the documentation or to certain reproduced product characteristics.
Safety Refer to the safety notes and instructions in the manuals of all necessary devices (e.g. microscope peripherals, cameras, computers, computer additionals, etc.) before installing and using the software.
Revision / Update Information 27.10.2003 Installation adapted from Product CD 07.2003 to the installation of the product CD number 15 of October 2003.
Table of Contents Table of Contents IMPORTANT NOTE ..................................................................................... 1 1 Installation of the AxioCam MRc5........................................................ 1 1.1 Safety Regulations........................................................................... 1 General notes .............................................................................. 3 Backup copies.......................................................................
Table of Contents Installing the camera driver using the setup program .................. 25 How to correct the driver entry in the device manager ... 29 Resetting the AxioCam MRc5 firmware ......................... 32 1.5 Notes on Maintenance .................................................................. 33 Camera Electronic ...................................................................... 33 Optical System ...........................................................................
Table of Contents Gamma setting .......................................................................... 63 Linearity of AxioCam MRc5 ........................................................ 63 Fluorescence images .................................................................. 63 Configurable toolbars for operating the camera.......................... 64 3 Trouble Shooting ................................................................................ 65 3.1 Software..................................
IMPORTANT NOTE FireWire (IEEE 1394) Interface The FireWire (IEEE 1394) interface is defined as a "hot plugging" interconnection, i.e. peripherals (cameras, drives, etc.) may be connected or disconnected while the computer is powered up. In connection with this "hot plugging" feature in isolated cases damages being caused to interface components were reported, presumably due to a discharge of static electricity during the insertion of the cables.
Installation of the AxioCam MRc5 1 Installation of the AxioCam MRc5 The AxioCam MRc5 is a professional camera scanner with FireWire interface for universal light-microscopy applications. To make it easier for you to set up the camera, please follow the instructions in these chapters step by step. In this manual the interface between AxioCam MRc5 and the computer is called FireWire. Synonym the terms IEEE 1394 and i.LINK are also used for this interface. 1.
Installation of the AxioCam MRc5 Limitation of liability No warranty shall be assumed by Carl Zeiss during the warranty period if the equipment is operated without observing the safety regulations. In any such case, Carl Zeiss shall be exempt from statutory liability for accidents resulting from such operation. Exemption from warranty Carl Zeiss shall be exempt from any warranty obligations should the user fail to observe the safety regulations.
Installation of the AxioCam MRc5 General notes Please ensure the notes described below are adhered to when setting up and operating the AxioCam MRc5 camera. All connectors must be firmly and securely attached. Please protect the data cable from excessive heat (e.g. halogen lamps, microscope fluorescence illumination). Only use the camera in a clean and dry location. The camera must be protected against mechanical impact. External damage may affect the operation of inner components.
Installation of the AxioCam MRc5 Backup copies We strongly recommend that all users save the data they create, such as images, measurement data, archives, reports, forms and documents, at regular intervals on an external medium. Otherwise it cannot be excluded that access to this data may be lost as a result of operational errors or hardware defects. Carl Zeiss accepts no liability for consequential damage resulting from insufficient data protection. 1.
Installation of the AxioCam MRc5 Recommended hardware and software Xeon workstation 2 GHz or faster 1 GB RAM 120 GB hard disk 1280 x 1024 bit graphics adapter for real color CD-ROM drive One free FireWire slot (OHCI compatibel, 400 Mbits/s transfer rate) respectively a free PCI slot (PCI Local Bus specification rev. 2.1 or higher) for the FireWire PCI bus interface 17" Multisync monitor Windows 2000 Professional Service Pack 3 or Windows XP Professional Service Pack 1 AxioVision software release 4.
Installation of the AxioCam MRc5 Contents of Delivery AxioCam MRc5 camera head FireWire data cable (6-pin to 6-pin) for power supply to the AxioCam MRc5 and for data transmission between camera and computer CD-ROM with device driver for AxioVision This Installation+Reference Guide Camera, data cable 6 B 48-0028 e 10.
Installation of the AxioCam MRc5 1.3 Setting up the Camera System The standardized FireWire interface allows the camera to be used for a variety of applications.
Installation of the AxioCam MRc5 The table below provides an overview of the order numbers of the accessories: Accessory Order Number FireWire hub 000000-0452-376 FireWire PCI bus interface card 000000-0452-036 FireWire PCcard (CardBus) interface card 000000-0452-037 The FireWire interface supports "hot plugging", i.e. the insertion and removal of the FireWire cable with the PC switched on.
Installation of the AxioCam MRc5 Operating the AxioCam MRc5 on a PC using the FireWire PCI bus interface card Installing the PCI bus interface card on the PC Static electricity can damage electronic components. To protect electronic components against static electricity, do not touch them until you have earthed yourself to the casing of the device. Never touch the contacts of electronic components. We also recommend that you work only on an antistatic mat.
Installation of the AxioCam MRc5 FireWire PCI bus interface card To ensure that power is supplied to the AxioCam MRc5, no other devices should be connected to the FireWire or USB ports of the FireWire PCI bus interface card. If you wish to operate other FireWire or USB devices, you can configure the FireWire PCI bus interface card in accordance with the manufacturer’s documentation. Switch off your PC and all connected peripherals. Disconnect the PC and the peripherals from the mains.
Installation of the AxioCam MRc5 Connecting the camera to the PC Lay the data cable carefully between the camera and the PC. Make sure that the cable is a safe distance from hot light sources on the microscope, to prevent it being damaged by heat. To connect the camera to a PC, you require a FireWire cable with a 6-pin connector at both ends. This is supplied with the camera. Connect the FireWire cable of the AxioCam MRc5 to the PCI bus interface card.
Installation of the AxioCam MRc5 Operating the AxioCam MRc5 on a PC with onboard FireWire interface Connecting the camera to the PC Lay the data cable carefully between the camera and the PC. Make sure that the cable is a safe distance from hot light sources on the microscope, to prevent it being damaged by heat. To connect the camera to a PC, you require a FireWire cable with a 6-pin connector at both ends. This cable is supplied with the camera.
Installation of the AxioCam MRc5 The AxioCam MRc5 has been designed for continuous operation. During operation the camera may heat up. This is quite safe, and does not in any way impair the functioning of the device.
Installation of the AxioCam MRc5 For installation of the FireWire PCcard (CardBus) interface card, please also take into account the notes in the interface card and notebook documentation. Only the basic principles of interface card installation are described in this manual. FireWire PCcard (CardBus) interface card Switch off your notebook and all connected peripherals. Insert the FireWire PCcard (CardBus) interface card into the appropriate slot on the notebook.
Installation of the AxioCam MRc5 The following components are required: FireWire hub with power supply (order number: 000000-0452-376) 6-pin to 6-pin FireWire cable (supplied with the AxioCam MRc5) to connect the AxioCam MRc5 to the FireWire hub. A second 6-pin to 6-pin FireWire cable (supplied with the FireWire hub) to connect the FireWire hub to the PCcard (CardBus) interface card. Connecting the AxioCam MRc5 to the FireWire PCcard interface card The camera is powered by the FireWire hub.
Installation of the AxioCam MRc5 Operating the AxioCam MRc5 on a notebook with onboard FireWire interfaces Connecting the camera to the notebook Lay the data cable carefully between the camera and the notebook. Make sure that the cable is a safe distance from hot light sources on the microscope, to prevent it being damaged by heat. To ensure the efficient use of energy resources, the FireWire slots on the notebook are not designed to supply power to peripheral devices.
Installation of the AxioCam MRc5 Connecting the AxioCam MRc5 to the onboard FireWire interface of the notebook The camera is powered by the FireWire hub. The hub must therefore be connected to the power supply. If you switch off the FireWire hub, the camera is also switched off. When the power supply of the FireWire hub is connected, the red LED of the FireWire hub lights up. The green LED of the FireWire hub lights up as soon as a FireWire device is connected.
Installation of the AxioCam MRc5 Mounting the camera onto the microscope To mount the camera onto your microscope’s TV port, use a 1.0x or a 0.63x C-mount adapter. Suitable adapters (example): 1.0 x 0.63 x Port 44 No. 452995 (44 C2/3" 1.0x) No. 452997 (44 C2/3" 0.63x) Port 60 No. 456105 (60 C2/3" 1.0x) No. 1069-414 (60 C2/3" 0.63x) For the Axiovert 25 please use adapter 451268. The adapters are not supplied with the camera.
Installation of the AxioCam MRc5 Second FireWire socket This FireWire socket may be used to cascade FireWire devices. This feature should only be used, if you are sure that the power specification of your computer's FireWire interface fits the needs of all connected devices. Expansion socket In addition to the socket for the data cable, the camera also has an additional socket. This socket can be used to open and close an external shutter via trigger output signals.
Installation of the AxioCam MRc5 1.4 Installing the Driver Software In order to operate the AxioCam MRc5 the AxioVision software is needed. First connect the AxioCam MRc5 to your PC (see the section "Setting up the camera system"), and then install the driver software. General Firstly we would like to emphasize a number of important points: Allow sufficient time for installation. As a rule this will take just over five minutes, although in exceptional circumstances considerably more time may be necessary.
Installation of the AxioCam MRc5 When installing the components it is possible that some of the files to be installed may already exist. You are informed if this is the case. You are then asked if you want to retain or overwrite the existing files. Always select the answers suggested by the installation program.
Installation of the AxioCam MRc5 This section describes installation using the hardware wizard. If you want to update the camera installation or if you have interrupted the driver wizard, follow the instructions in chapter "Installing the camera driver with the setup program". Once you have connected the AxioCam MRc5, the Found New Hardware Wizard dialog appears on your screen. Please ensure that during all the steps below, the CD-ROM containing the AxioCam MRc5 driver is in your PC’s CD drive.
Installation of the AxioCam MRc5 Select the option Search for a suitable driver for my device (recommended). Click Next. Activate the check box CD-ROM drives. Click Next. The following dialog window shows the actual path of the AxioCam MRc5 driver. Click Next.
Installation of the AxioCam MRc5 Under Windows 2000 Professional this dialog window is displayed. Click Yes to continue. Under Windows XP Professional the Hardware Installation dialog window is displayed. In this window click on Continue Installation. The last dialog window of the hardware wizard is displayed. Click Finish to close the Hardware Wizard. To complete the installation follow the steps in the next chapter. 24 B 48-0028 e 10.
Installation of the AxioCam MRc5 Installing the camera driver using the setup program NOTE: For installation under Windows 2000 Professional and Windows XP Professional, you need to log on with administrator privileges. If you do not have access to administrator rights on your computer, please speak to your system administrator. In this section, installation is explained using screenshots from Windows 2000 Professional. However, the procedure is the same for Windows XP Professional.
Installation of the AxioCam MRc5 If the automatic start function has been switched off on your system (Autorun) you must start the installation program manually in the following way: Double-click on the desktop icon My Computer Double-click on the CD-ROM Drive icon Double-click on the entry setup.exe After the start procedure (automatically resp. manually) this image will be displayed on your monitor: Select the language you want to install (English or German) and click OK.
Installation of the AxioCam MRc5 Under Windows 2000 Professional this dialog window will be displayed during installation of the AxioCam MRc5. Click Yes to continue the installation. Under Windows XP Professional the Hardware Installation dialog window is displayed. In this window click on Continue Installation. Once the AxioCam MRc5 driver has been installed, the installation program checks the camera’s firmware.
Installation of the AxioCam MRc5 Start the systems Device Manager to check the installation: Click Start ⇒ Settings ⇒ Control Panel. Double-click the System icon. The System Properties dialog will be displayed. Select the property page Hardware and here the Device Manager button. Now double-click the entry Imaging devices to check the entry Carl Zeiss AxioCam MRc5. 28 B 48-0028 e 10.
Installation of the AxioCam MRc5 How to correct the driver entry in the device manager NOTE: In this chapter the correction of the driver entry is described with Windows 2000 Professional screen shots, but the procedure is similar under Windows 2000 Professional and Windows XP Professional. If the entry in the Device Manager must be corrected, i.e. if a question mark or an exclamation mark is in front of the AxioCam MRc5 entry, proceed as follows.
Installation of the AxioCam MRc5 The property page General opens. In this case, reinstall the AxioCam MRc5 using the installation program (see section "Installing the camera driver using the setup program"). 30 B 48-0028 e 10.
Installation of the AxioCam MRc5 After the camera driver has been installed and (depending on the operating system you are using) your computer has been restarted, the property window now contains the following entry: The entry in the Device Manager is now Carl Zeiss AxioCam MRc5.
Installation of the AxioCam MRc5 Resetting the AxioCam MRc5 firmware If the color of the LED on the back of the AxioCam MRc5 does not immediately change from red to green but goes out after the insertion of the FireWire cable, this means that the AxioCam MRc5’s firmware is not functioning properly. This error can occur, for example, if the data transmission or power supply to the camera has been cut off during the firmware recording procedure.
Installation of the AxioCam MRc5 1.5 Notes on Maintenance Camera Electronic Camera, interface and power supply are maintenance free. Optical System The internal optical components of the camera should always be protected. If no lens or TV adapter incorporating optics is screwed into the camera's CMount thread, the camera's sensor and IR-filter must be protected by screwing the protective cap into the camera's C-Mount thread.
Image Acquisition with the AxioCam MRc5 2 Image Acquisition with the AxioCam MRc5 2.1 General The AxioCam MRc5 is a professional digital camera with a high-resolution 5 megapixel CCD color sensor for universal application in the field of light microscopy. The color camera is suitable for all situations where stained or naturally colored samples need to be acquired quickly and with very high camera resolution.
Image Acquisition with the AxioCam MRc5 2.2 Quick Guide to the First Image with AxioVision and AxioCam MRc5 If you are using several cameras on your system you must always make the AxioCam MRc5 camera the active camera before proceeding. Start AxioVision by double-clicking on the corresponding symbol. Select from the Acquisition menu the Select AxioCam MRc5 function. The image acquisition can also be easily done via the toolbar. Open the corresponding menu by clicking on .
Image Acquisition with the AxioCam MRc5 The Workflow Standard Live starts and closes the live window. Show live window properties opens and closes the window for adjusting the display characteristic curve and for controlling the camera (exposure time, white balance etc.). Snap acquires an image using the active camera. Show Properties adapts the image display using a characteristic curve and shows the parameters of the image. Scale bar inserts a scale bar.
Image Acquisition with the AxioCam MRc5 Step by step to the first image Select the camera you want to use (if only one camera is installed, this step is not necessary). Set the light path to the camera. Then click on the live image icon. The live window is opened to display the camera image. This function can also be called up via the Acquisition ⇒ Live menu. Now focus the camera image and select the frame you want to acquire.
Image Acquisition with the AxioCam MRc5 Here you can see the settings for the display of the image like Brightness, Contrast and Gamma. Settings made here are passed on the acquired image. NOTE: Only the display is adapted, the camera data remain unchanged. 38 B 48-0028 e 10.
Image Acquisition with the AxioCam MRc5 Make changes for the camera settings on the following controls: The central element is the setting for the exposure time on a digital camera. The Measure button can be used to determine an optimum exposure time. Check the quality of the exposure in the live image. The arrow keys and sliders can be used for fine adjustment of the exposure time. As a color camera the AxioCam MRc5 needs to perform a white balance for the image.
Image Acquisition with the AxioCam MRc5 Move the sliders Gamma Contrast Brightness up or down to adjust the image. Good color reproduction on a monitor is achieved with a gamma value of around 0.45. To find the optimum setting for contrast and brightness, click on Min/Max. To undo all your changes, click on Linear. You can use this icon to insert a scale bar into your image. The appropriate scaling must have been generated and selected in advance.
Image Acquisition with the AxioCam MRc5 Captions and markings can be inserted using the Annotations window. Click on this icon to save the image you have generated to the computer’s hard drive. NOTES: Once you have found a good camera setting, you can save it with the Settings Editor in the Tool menu and load it again automatically in the future. This allows you, for example, to acquire particular samples under the same conditions every time.
Image Acquisition with the AxioCam MRc5 2.3 Adjust Property Page This property page contains a summary of the most important camera settings, which are constantly used during operation. The property page is splitted in the fields Exposure, White Balance (with color settings) and Histogram (for the display of an image histogram). 42 B 48-0028 e 10.
Image Acquisition with the AxioCam MRc5 Exposure The camera’s exposure time can be adjusted to the lighting conditions on the microscope using a slider or by entering an exposure time directly into the input field. When entering the exposure time manually you can state the value range directly using the abbreviations "ms" for milliseconds and "s" for seconds.
Image Acquisition with the AxioCam MRc5 A setting of 200% means that 200% of the exposure time set in the display window or measured by pressing the appropriate button is used for actual acquisition. A setting of 50% means that 50% of the exposure time set in the display window or measured by pressing the appropriate button is used for actual acquisition. The setting range is between 5% to 500%. EXAMPLE: A thick sample is acquired in bright field.
Image Acquisition with the AxioCam MRc5 White Balance In order to achieve a color display which is neutral and close to reality it is necessary to balance the individual color channels against one another. For this, you need to use the pointer to click in the live image on an area of the microscope’s specimen, which is white, neutral and illuminated as evenly as possible. The scene must not be overexposed. It is important to set the exposure correctly before carrying out the white balance.
Image Acquisition with the AxioCam MRc5 Color adjustment Clicking on the buttons and allows you to change the weighting of the individual color channels manually. Using this method a white balance you have generated can be subsequently changed. Each click on the arrows shifts the weighting of the colors in the direction indicated. These changes can be observed immediately in the live image and in the histogram display.
Image Acquisition with the AxioCam MRc5 If an image is underexposed, the histogram does not reach the right-hand edge of the display area. As a result optimum use is not made of the camera’s resolvable gray value range. You should therefore increase the exposure time or the amount of light in the microscope. Log: By clicking on this button it is possible to influence the histogram display. You can toggle between a linear scaling and a logarithmic display of the signals represented in the histogram.
Image Acquisition with the AxioCam MRc5 2.4 Frame Property Page The Frame property page can be used to select a frame for the image acquisition and is splitted in the fields Camera Mode and Frame. Camera Mode RGB and B/W: With the AxioCam MRc5 you can use these setting options (RGB or B/W) to determine whether the camera should supply a color or a black and white image. This may be desirable for fluorescence images, or to 48 B 48-0028 e 10.
Image Acquisition with the AxioCam MRc5 reduce the amount of data. The camera will always acquire a color image, which is then converted to a black and white image. Depending on the selected mode certain binning modes are available. Acquisition modes The AxioCam MRc5 essentially offers two acquisition modes: "Quality" mode and "Fast" mode. These modes are determined by the technical functioning of the sensor.
Image Acquisition with the AxioCam MRc5 Selectable resolution in "Quality" mode ("Iinterlaced") – recommended for highest resolution of detail: H V 516 x x 387 Binning 5x5 646 x 484 4x4 861 x 645 3x3 1292 x 968 2x2 2584 x 1936 Standard single exposure at sensor’s highest basic resolution (All modes in color) Selectable resolution in "Fast" mode ("Progressive") – recommended for moving objects: H 258 x x V 193 Binning 10x10 Color Color 323 x 242 8x8 Mono 430 x 322 6x6 Col
Image Acquisition with the AxioCam MRc5 Binning The process of Binning ensures that the information in neighboring pixels, in both a horizontal and vertical direction, is summarized on the camera's sensor. This increases image brightness in accordance with the number of pixels summarized. However, this process also reduces the sensor's spatial resolution accordingly. Since the amount of data to be transferred is reduced, the potential image rate is increased.
Image Acquisition with the AxioCam MRc5 Frame In this field you can specify whether the frame or the entire area of the sensor is used for the acquisition. Refresh overview: This button makes it possible to insert an overview image into the area where the frame can be positioned. This makes it extremely easy to target your specific area of interest. In the displayed overview image a frame can be positioned by using the mouse, which corresponds to the selected frame of the acquired image.
Image Acquisition with the AxioCam MRc5 2.5 General Property Page On this property page you enter the basic settings for the camera. Digital Gain Using the slider it is possible to change the interpretation of the brightness of the image data received. This allows you to display dark images as very bright images, which means you can acquire images with very high contrast using short exposure times.
Image Acquisition with the AxioCam MRc5 The disadvantage of this technique, however, is that noise is greatly intensified, and the potential dynamic range of the corresponding image data is reduced. You should therefore carefully weigh up the benefits of using digital signal gain before actually employing the technique. For this reason the recommended value is gain by a factor of 1. From a technical viewpoint, this technique involves multiplying the data by a corresponding factor.
Image Acquisition with the AxioCam MRc5 For this calibration process make sure that the camera is viewing a completely black image. For example, close the light path to the camera on the microscope. In exceptional cases it may be necessary to remove the camera from the microscope for this calibration process and to cover the sensor with the cap provided. Calibration takes approx. 20 seconds. At the end of this period it will continue to be available even if the software is restarted.
Image Acquisition with the AxioCam MRc5 Shutter Control Via a supplementary cable the AxioCam MRc5 offers a TTL control pulse, which can be used, for example, to control a mechanical shutter (e.g. "Uniblitz") in synchronism with the camera’s exposure time.
Image Acquisition with the AxioCam MRc5 Enhance Color: Activating this check box enables you to switch the color optimization algorithm on or off. Under normal circumstances this check box should always be activated in order to obtain vivid color reproduction during image acquisition. If it is deactivated the noise in the image is reduced and the speed of the live image increased. NOTE: For the Gamma curve a value of approx.
Image Acquisition with the AxioCam MRc5 Deactivating the focus bar. Deactivating the live image scaling (1:1 representation of the image). Activating a frame for the live image (Size) on the Frame property page. Set image orientation to original on the General property page (no rotation). NOTE: Note that the above mentioned notes are only valid for short exposure times, as for very long exposure times the speed of the acquisition is mainly dominated by the exposure time.
Image Acquisition with the AxioCam MRc5 This adjusts the display characteristic curve automatically for an optimum color reproduction of the monitor (gamma = 0.45). In the Properties dialog window, set the gamma characteristic curve in the histogram image on the Display property page. NOTE: This also influences the reproduction of the color saturation. For fluorescence images, background correction can also be carried out simply by shifting the offset of the characteristic curve.
Image Acquisition with the AxioCam MRc5 You can now select the area to be displayed by dragging the black rectangles along the characteristic curve or carry out automatic optimization by clicking on the corresponding buttons. A histogram of the gray and color values will be displayed which makes the gray and color distribution visible. The Best Fit, Min/Max and Linear buttons can be used to quickly adjust the histogram display. You can influence the display by clicking on the Log or Skip buttons.
Image Acquisition with the AxioCam MRc5 Best Fit calculates in the current histogram the values which exclude 1‰ of the pixels contained in the image. The color/color range calculated in this way is displayed on the screen. (You can adjust the value of 1‰ to suit your requirements). Min/Max displays on the screen the smallest and largest color or color value present in the image. Linear displays on the screen the total possible value range.
Image Acquisition with the AxioCam MRc5 Recommended camera settings Activate the Enhance Color check box on the General property page to improve the color reproduction quality (this slows down the live image rate on older PCs slightly as additional calculations are required). The standard setting installed for 3200 K (3200 K button on the Adjust property page) often offers the camera the most natural reproduction of the image situation on the microscope.
Image Acquisition with the AxioCam MRc5 2.8 Background Information Gamma setting This setting is required to compensate for the inherently non-linear behavior of the image tube ("gamma"). It also influences the quality of the color display. You can get the best display of colored objects, if the gamma setting is in the range of approx. 0,45. Many video cameras have built-in gamma compensation, as they are intended for use on video monitors.
Image Acquisition with the AxioCam MRc5 In the case of fluorescence, however, only very narrow-band light is emitted. This is received only by the color pixels that are sensitive to it. Consequently, the resolution of images of this type is reduced in comparison with white light. As fluorescence samples can be subject to bleaching during exposure, there is a risk that the image intensity of images in “quality” mode may diminish from one component image to another.
Trouble Shooting 3 Trouble Shooting If errors occur, check first the setup instructions of the camera. 3.1 Software Please also pay attention to all the comments in the file ReadMe.TXT, which is displayed during the driver installation. AxioCam MRc5 does not appear in the menu of selectable cameras Make sure that the AxioCam MRc5 files have been installed in accordance with the installation of the software.
Trouble Shooting 3.2 Image Quality Sudden jolt, vibration or moving objects If the microscope is jolted whilst you are taking a shot, the image will show periodic interference. Repeat the shot and take care to ensure the conditions remain calm. Do not use the "Quality" mode for moving objects. Fluctuations in illumination If the light source has a fluctuating quality of light intensity, stripes or blotches will show up in the image.
Technical Data 4 Technical Data 4.1 AxioCam MRc5 Number of pixels: 2584 (H) x 1936 (V) = 5 Megapixels Digitization: 12 bit / 12 Mhz pixel clock Dynamic range: Typical 1:1300 Integration time: 1 ms to 60 s Cooling: Single stage Peltier cooling Interface: FireWire / IEEE 1394, 6-pin socket, speed 400 Mbit Live image frame rate: Mode H x V Max.frame rate Slow 1296 x 968 3 frames/s Medium 430 x 322 11 frames/s Fast 258 x 193 16 frames/s Note: Max.
Technical Data Pixel size: 3,4 µm x 3,4 µm Sensor size: 8,7 mm x 6,6 mm entspricht 2/3" CCD Sensor: ICX 282, Interline Transfer Spectral range: Approx. 400 nm to 710 nm with infrared barrier filter BG40 Read-out modes: "Progressive" / "Interlaced" Control signals: TTL-output for controlling external electrical shutters Optical interface: C-mount (17,5 mm) Thread depth for objectives: Maximum of 5 mm (C-mount) Max. file size per image: Approx.
Technical Data 4.2 Spectral Sensitivity Standardized at maximum amplitude It does not make sense to operate the AxioCam MRc5 (color version) without an infrared barrier filter, as color is only defined for the visible wavelength range.
Index Index control signal 56 D 1 12-bit images 59 A acquisition delay 56 acquisition modes 49 Adjust property page 42 automatic exposure 44 automaticalle adjust white balance 45 B background information 63 backup copies 4 basic settings 53 binning 51 black reference 54 brightness, change 61 C camera adaption 50 camera electronic 33 camera mode 48 camera settings 42, 62 camera system 7 center frame 52 C-mount objectives 33 color adjustment 46, 57 color channel balance 45 color reproduction 58 color value
Index How to correct the driver entry in the device manager 29 optimum color reproduction 58 overexposure 47 I P image acquisition 34 image frame 48 image orientation 54 image properties 59 image quality 66 infra-red barrier filter 33 installing the camera driver using the setup program 25 installing the camera driver with the hardware wizard 21 installing the driver software 20 PCcard interface on the notebook 13 PCI bus interface card 9 practical notes on operation 57 property pages Adjust 42 Frame
Index system requirements 4 T technical data 67 toolbars 64 trouble shooting 65 U underexposure 47 W warranty 2 weighting of exposure time measurement 43 white balance 45 white reference 55 Workflow 36 AxioCam MRc5 Installation + Reference III
