User manual

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Leica FluoCombi III™ – Appendix
4.1 Filter blocks for macro-fluorescence
4. Appendix
Filter block Fluorochrome Excitation Excitation filter Dichromatic Suppression Order no.
Mirrors filter
A various UV BP 340-380 400 LP 425 11 513 804
+ A4 various UV BP 360/40 400 BP 470/40 11 513 839
D various UV / violet BP 355-425 455 LP 470 11 513 805
E4 various violet / blue BP 436/7 455 LP 470 11 513 806
H3 various violet / blue BP 420-490 510 LP 515 11 513 807
I3 various blue BP 450-490 510 LP 515 11 513 808
K3 various blue BP 470-490 510 LP 515 11 513 809
+ L5 various blue BP 480/40 505 BP 527/30 11 513 840
M2 various green BP 546/14 580 LP 590 11 513 811
N2.1 various green BP 515-560 580 LP 590 11 513 812
+ N3 various green BP 546/12 565 BP 600/40 11 513 841
+ TX2 TEXAS RED green BP 560/40 595 BP 645/75 11 513 843
+ Y3 CY3 green BP545/30 565 BP 610/75 11 513 837
+ Y5 CY5 red BP 620/60 660 BP 700/75 11 513 844
+ Y7 CY7 red BP 710/75 750 BP 810/90 11 513 845
+ GFP Green-FP blue BP 470/40 500 BP 525/50 11 513 847
+ YFP Yellow-FP blue BP 500/20 515 BP 535/30 11 513 863
+ Red GFP Red-FP green BP 546/12 560 BP 605/75 11 513 864
+ CGFP Cyan-FP violet / blue BP 436/20 455 BP 480/40 11 513 862
Empty system* 0 0 0 11 513 842
BP bandpass filter, wavelength in nm
LP long-pass filter, flank wavelength in nm
+ Specifically for multi-parameter fluorescence
* for customer-specific instrumentation
On principle, it is possible to arrange filter combination accord-
ing to customer information (with filters from other manufactur-
ers) so that they meet the requirements according to 0-pixel
shift (ch. 3.8). This requires that the third-party products meet
certain minimum quality requirements.