User manual

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3.8 Incident light fluorescence illuminator
The patented Leica zero-pixel-shift technology
ensures an exact convergence for the superposi-
tion of different fluorescences. When the filter
blocks are switched, no interfering pixel offset
occurs between the individual images of the dif-
ferent spectral ranges – an ideal prerequisite for
digital multicolor fluorescence experiments.
The illumination is coupled into the zoom optics.
This unique innovation from Leica Microsystems
guarantees exact light guide, maximum light
utilization and homogenous fields of view in any
zoom position.
Fig. 14 Incident light fluorescence
illuminator LRF 4/22
1 Nosepiece disc with 4 positions
2 Adjustment of field diaphragm F
3 Adjustment of aperture diaphragm A
4 Light stop (dark flap) STOP
3.8.1 Field diaphragm
The field diaphragm is used for reflection protec-
tion and specimen protection.
왘 Open the field diaphragm F (14.2) until the
complete field of view is illuminated.
3 = open
To protect sensitive fluorescence specimens
Restrict the field diaphragm (14.2) as needed.
Centering the field diaphragm
왘 Insert the supplied centering keys in the two
centering openings (14.6)
왘 Turn the centering key until the diaphragm is
centered.
5 Blue filter BG 38
6 Sockets for centering keys (field diaphragm)
7 Sockets for centering keys (aperture
diaphragm)
Leica MacroFluo – Operation
1
2
7 3 4 5
6