User manual

22
3.7 Adjusting diopter settings (parfocality)
With adjustable eye lenses at the eyepieces, the
observer can compensate his or her defective
vision. The correct diopter compensation is
required for both eyes so that focusing remains
constant during zooming (parfocal). With a cor-
rect setting, you no longer have to refocus during
zooming.
If you turn the eye lens, an engraved silver
line running all around on the outside of the eye-
piece housing becomes visible. It indicates the
correct position of the eye lens for normal eye-
sight and for users wearing glasses during
microscopy work with glasses.
Diopter compensation for defective vision
We recommend to perform the following
settings in transmitted light or incident light.
왘 Activate transmitted or incident light at the
light source.
If the fluorescence illumination is already
activated at the supply unit and the burner
is adjusted:
왘 Switch filter turret to an empty position (14.1) or
close the light stop (14.4).
왘 Set up the zoom system in the working distance
(13.5) of the objective used (see the table on
p. 30).
왘 Set the eyebase (13.1).
왘 Fully OPEN the iris diaphragm (13.6).
왘 Turn the eye lenses (13.2) until the rotating part
reaches the line engraved in the eyepiece
housing.
While adjusting the eye lenses, securely hold
the eyepiece at the bottom of the knurled ring.
왘 Set the lowest zoom position at the magnifica-
tion changer (13.7).
왘 Illuminate a flat test object with sharp contours
using transmitted light or incident light and
focus it using the focusing drive (13.8, shown
with motor focus).
왘 Set the highest zoom position and optimize
image sharpness with focusing drive.
왘 Set the lowest zoom position at the magnifica-
tion changer (13.7).
왘 Individually adjust the image sharpness for
each eye by turning the eye lenses (13.2):
– For example, first observe the object with the
left eye while closing the right eye. Slowly turn
the left eye lens until you can sharply see the
test object with the left opened eye.
– Now close your left eye and adjust the eye lens
for the right eye.
왘 Set the highest zoom position at the magnifi-
cation changer (13.7).
왘 If necessary, use the focusing drive (13.8) to
fine-focus again.
왘 Set the lowest zoom position at the magnifi-
cation changer (13.7).
왘 Check image sharpness and parfocality: Slowly
zoom to the highest zoom position (13.7).
• The image sharpness will now remain constant
at all magnification levels, without the need to
refocus with the focusing drive.
If this is not the case, repeat this procedure.
Leica MacroFluo – Operation