YSI 5905, 5010, 200-BOD, and Pro-BOD Probe Instruction Manual
TABLE OF CONTENTS INTRODUCTION ................................................................ 1 OPERATING PRINCIPLES ....................................................... 2 USING THE PROBE ............................................................. 3 Changing the Membrane Cap.................................................... 3 Warm Up..................................................................................... 5 Electrode Cleaning..............................................................
INTRODUCTION The YSI Model 5905/5010/200-BOD/Pro-BOD (Biochemical Oxygen Demand) probe is used for measuring dissolved oxygen in all popular size BOD bottles. These probes feature self stirring, easily replaceable membrane caps, and a refurbishable electrode system. The 5905 probe is designed for use with all YSI grey-box-style dissolved oxygen meters, except for the Model 55 or Model 550A. It must be used with an adapter (5011) in order to be used on the Model 5000 and 5100 bench top instruments.
OPERATING PRINCIPLES The 5905/5010/200-BOD/Pro-BOD probe is a polarographic dissolved oxygen sensor. An oxygen permeable membrane covers an electrolytic cell consisting of a cathode and an anode. This membrane acts as a diffusion barrier and an isolation barrier preventing the fouling of the cathode surface by impurities in the environment. The cathode is the gold electrode, and the anode is the silver electrode which completes the electrolytic cell and acts as a reference electrode.
USING THE PROBE The 5905/200-BOD/Pro-BOD must be plugged into a YSI meter and the stirrer power plug must be plugged into its power adapter. The 5010 must be plugged into a YSI meter only, as it gets its stirring power from the 5000/5100 instrument. Changing the Membrane Cap ---IMPORTANT--New Probe Use New probes are shipped with a dry membrane cap to protect the electrodes. A new membrane cap must be installed before first use. For the 5905/5010, use a Teflon membrane from a 5906 Membrane Kit.
Remove Stir Paddle Fill Membrane Cap with Solution Unscrew Cap Replace Stir Paddle Screw Cap on moderately tight Figure 1: Membrane Installation on the 5905/5010/200-BOD/ProBOD .
Warm Up Every time the instrument is turned on, the 5905/5010/200BOD/Pro-BOD Probe must be allowed to warm up for about 1020 minutes before taking the first reading. This allows the probe to use up all the oxygen retained in the cap. ---IMPORTANT--New Probe Break In (motor off) For best results, please allow the probe more than the usual 20 minutes to warm up after the first membrane is installed. The process may be faster if the probe is allowed to warm up in a BOD bottle filled with clean water.
Silver Anode It is normal for a dark layer of silver chloride to cover the silver anode (for its location, see figure 2). After prolonged use it may become necessary to clean the anode. Soak the probe in a 14% ammonium hydroxide solution for 2 to 3 minutes or overnight in a 3% ammonium hydroxide solution. Rinse with deionized water, recharge with electrolyte, and install a new membrane. The anode may also need a very light sanding with the supplied sanding discs.
OPERATING PROCEDURES AND PRECAUTIONS ---IMPORTANT--Intended Use The 5905/5010/200-BOD/Pro-BOD probe is intended for laboratory use, not field use. It is not waterproof and should never be immersed past the taper on the stem of the probe. Air Calibration When calibrating in air, make sure there are no water droplets on the membrane. Water droplets will cause a low calibration reading. Excess water may be removed by shaking the probe downward.
The average replacement interval is two to four weeks. Probes in constant or heavy use may require more frequent membrane changes. Interferences Hydrogen sulfide, sulfur dioxide, halogens, carbon monoxide, chlorine, nitric oxide, and nitrous oxide can cause the probe to give erroneous readings. If you suspect erroneous readings, it may be necessary to determine if these are the cause.
PROBLEM SOLVING Trouble Shooting Guide Use this quick reference table to correct symptoms you might be experiencing. Symptoms Unable to calibrate Actions • Allow approximately 20 min. and up to 30 min.
Symptoms Unstable readings Actions • Allow sufficient time for temperature and oxygen stabilization • Allow 60 sec for reading to stabilize • Change membrane • Clean probe (see instructions under Electrode Cleaning) Motor not working • Check power supply • Manually turn the stir paddle to help start the motor • Replace motor Loud motor • Check if stir paddle is pushed all the way in • Replace motor Membrane cap is tight 10 • Use pliers to loosen the cap (turn counter clockwise while facing senso
SERVICING The 5905/5010/200-BOD/Pro-BOD has four user replaceable parts. The motor assembly can be replaced if it becomes noisy, unbalanced, or malfunctions. The probe assembly, housing assembly, and stir paddle can also be replaced if they become damaged. To disassemble the probe, perform the following steps while referring to figure 3: 1. Remove stir paddle by pulling it straight out. 2. Remove the two screws (A) on the outside of the housing using a 3/32 Allen wrench. 3.
(B) Housing Assembly (A) (A) (C) (C) (D) (D) Motor Assembly Probe Assembly Stir Paddle Figure 3: Exploded View of 5905/5010/200-BOD/Pro-BOD Probe 12
WARRANTY AND REPAIR YSI Models 5905/5010/200-BOD/Pro-BOD Dissolved Oxygen Probes are warranted for one year from date of purchase by the end user against defects in materials and workmanship. Electrode cleaning or maintenance is not covered under warranty. Within the warranty period, YSI will repair or replace, at its sole discretion, free of charge, any product that YSI determines to be covered by this warranty.
Authorized Service Centers YSI has several Authorized Service Centers in the U.S. and around the world. Please refer to the YSI web site (www.YSI.com) for your nearest Authorized Service Center. Cleaning Instructions NOTE: Before they can be serviced, equipment exposed to biological, radioactive, or toxic materials must be cleaned and disinfected. Biological contamination is presumed for any instrument, probe, or other device that has been used with body fluids or tissues, or with wastewater.
decontamination has been accomplished and that no radioactivity is detectable by survey equipment. 4. Any product being returned to the YSI Repair Center should be packed securely to prevent damage. 5. Cleaning must be completed and certified on any product before returning it to YSI. Packing Instructions 1. Clean and decontaminate items to insure the safety of the handler. 2. Complete and include the Cleaning Certificate. 3. Place the product in a plastic bag to keep out dirt and packing material. 4.
Alternatively, if you are planning to send your probe directly to YSI for servicing, please visit www.ysi.com, download and fill out the Product Return Form.
Probe Specifications Cathode: Gold Anode: Silver Membrane: FEP Teflon (for 5905/5010) Polyethylene (for 200-BOD/Pro-BOD) Dissolved Oxygen Accuracy: ± 0.1 mg/L or ± 2% of reading, whichever is greater Operating Temperature Range: 0° to 45°C Temperature Accuracy: ± 0.2°C Polarizing Voltage: 0.8 volts Response Time: Typical response to a step change in dissolved oxygen at 20°C is 90% of the change in 30 seconds.
APPENDIX A The 5905 and 5010 probes come in several versions, designed to fit different bottle sizes. 5905 5010 200-BOD Pro-BOD 5905 - J 5010 - J 5° taper per side to fit U.S. Standard BOD bottles. Includes an adapter to fit 5° taper per side smaller bottles with built - in overflow funnels. 5905 - K 5010 - K Includes a 19 funnel. 5905 - L 5010 - L Includes a 24 funnel. 5905 - W 5010 - W 6″ long probe for use with wine bottles.
19 5905 059880 N/A 059895 110590 110592 110591 N/A 073035 073047 Description 5906 Membrane Kit 5908 Membrane Kit Stir Paddle Probe Assembly Motor Assembly Housing Assembly Funnel / Adapter 115 V Power Supply 230 V Power Supply 073047 073035 059929 110591 110592 115904 059902 N/A 059880 5905-J See dealer See dealer 059933 110591 110594 115904 059902 N/A 059880 5905-K 073047 073035 059911 110591 110594 115904 059902 N/A 059880 5905-L 073047 073035 N/A 1105
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