User manual

Section 7- Diagnostics and Troubleshooting
7. Diagnostics and Troubleshooting
The Diagnostics task bar accesses both the Intensity Check and the Calibration Check. The Intensity Check
confirms that the internal spectrometer is functioning as expected and the Calibration Check confirms that the
instrument is performing within the pedestal pathlength calibration specifications.
Intensity Check
With the sampling arm down, click Measure to perform an Intensity Check.
If yellow triangles are displayed in the top left of the spectral display for either the Visible or UV indicator, please
clean the pedestal as described below, and then repeat the Intensity Check.
A
Cleaning and Reconditioning Guide can be accessed from the “Appendices” chapter of the NanoDrop 2000 User
Manual PDF. It is recommended that this guide be printed and posted near the instrument as a reference.
1. Apply 3-5 µL of dH
2
O onto the bottom pedestal.
2. Lower the upper pedestal arm to form a liquid column; let it sit for approximately 2-3 minutes.
3. Wipe away the water from both the upper and lower pedestal with a clean, dry lab wipe.
If a yellow triangle is displayed in the top left of the spectral display for the Bias indicator, please ensure that the
instrument is not located in a cold environment. Allow the unit to warm up to room temperature to repeat the
Intensity check.
If problems persist, email the Intensity check results to
Technical Support. Outside of the US and Canada, please
contact your local NanoDrop products distributor. The spectra are automatically saved as .JPG files at the following
location:
Vista: C:\Users\Public\Public Documents\Thermo\Autosave\IntCheck
XP: C:\Documents and Settings\AllUsers\Documents\Thermo\NanoDrop2000\Autosave\IntCheck
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