User manual

Section 6- Warranty and Maintenance

Pedestal Reconditioning

The Bradford reagent as well as other buffers containing surfactants may “un-condition” the measurement pedestal

surfaces so that the liquid column does not form well with 1 µL samples. Use the NanoDrop Pedestal

Reconditioning Compound (PR-1) as a rapid means of reconditioning the pedestals when the surface properties

have been compromised and liquid columns break during measurement. This will “re-condition” the surfaces

allowing the liquid sample column to form.

Procedure

1. Open the vial containing PR-1 and use the applicator provided in the kit to remove a pin-head sized amount

of the compound.

2. Apply a very thin, even layer of PR-1 to the flat surface of the upper and lower pedestals.

3. Wait 30 seconds for the PR-1 to dry.

4. Fold a clean, dry laboratory wipe into quarters and remove the PR-1 by rubbing the surface of the upper

and lower pedestals until all compound residue is removed.

Note: The black appearance of the removed residue on the laboratory wipe is normal.

The reconditioning process is complete once the laboratory wipe no longer shows black residue. Use canned air to

remove excess lint from the diaphragm.

To check the effectiveness of the reconditioning, pipette a 1 µL aliquot of dH

O onto the lower measurement

pedestal and visually verify that the water “beads” up. Additional information about the PR-1 kit may be found on our

website.

Droplet “flattens” out on Droplet “Beads” up on

un-conditioned pedestal properly conditioned pedestal

As an alternative to using the PR-1 Kit, the pedestals may be reconditioned as follows:

1. Fold a clean dry lab wipe over several times to increase its thickness.

2. Press the lab wipe firmly down on the lower pedestal and "buff/rub" aggressively at least 50 times. The lab

wipe may rip during this process and may need to be refolded several times. The upper pedestal should

also be buffed but care should be taken not to put too much force on the upper arm.

3. Use canned air to remove any excess lint from the diaphragm.

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