User manual

Section 6- Warranty and Maintenance
Maintenance of the Sample Retention System
Cleaning
The primary maintenance requirement for the NanoDrop 2000/2000c is keeping the measurement pedestal
surfaces clean. Upon completion of each sample measurement, wipe the sample from the upper and lower
pedestals with a clean, lint-free lab wipe to prevent sample carryover and avoid residue buildup.
Although generally not necessary, 2 µL water aliquots can be used to clean the measurement surfaces after high
concentration samples to ensure no residual sample is retained on either measurement surface.
1. Apply 3-5 µL of dH
2
0 on to the bottom pedestal.
2. Lower the upper pedestal arm to form a liquid column; let it sit for approximately 2-3 minutes.
3. Wipe away the water from both the upper and lower pedestal with a clean lab wipe.
- Between measurements
Upon completion of each sample measurement, wipe the sample from both the upper and lower
pedestals with a clean, dry, lint-free lab wipe, to prevent sample carryover and avoid residue buildup. It is
not necessary to clean with dH
2
O between samples.
- Between users
A final cleaning of both measurement surfaces with dH
2
O is recommended before the first sample
measurement and after the last sample measurement.
- Additional cleaning
When a more rigorous cleaning protocol is required (i.e. dried proteins) substitute 0.5M HCl for the dH
2
O
in the procedure above. Follow with 3- 5 µL of dH
2
O.
- Decontamination
A sanitizing solution, such as a 0.5% solution of sodium hypochlorite (1:10 dilution of common
commercial bleach solutions – freshly prepared), may be used to ensure that no biologically active
material is present on the measurement pedestals. Follow with 3- 5 µL of dH
2
O.
The use of detergents or isopropyl alcohol is not recommended as they may un-condition the pedestal
measurement surfaces. If a solution containing either is used, it is important to follow with 3- 5 µL of dH
2
O.
Note: Do not use a squirt or spray bottle to apply dH
2
O or any other liquid to the surface of the instrument.
A single page
Cleaning and Reconditioning Guide can be accessed from the “Appendices” chapter of the NanoDrop
2000 User Manual PDF. It is recommended that this guide be printed and posted near the instrument as a
reference.
The diaphragm around the pedestal is permanently affixed to the instrument. Do not attempt to remove the
diaphragm for cleaning purposes. Avoid prolonged exposure of the diaphragm to HCl, alcohol, bleach, acetone or
other solvents as the adhesive underneath may be affected. If the diaphragm comes loose- please contact
Technical Support. Outside of the US or Canada, contact your local NanoDrop products distributor.
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