User manual

Section 5-Kinetics
Note: Rate vectors are by default locked to the curve and will display the best fit for the two time points
selected. Highlight the vector and right-click to deselect the Lock to curve feature to manually
move the vector.
The data for the rate vector is shown in a table to the right of the Rate display. Fields include:
• Vector Name - name of the rate vector.
• Time 1 - beginning time of the rate vector.
• Time 2 - end time of the rate vector.
• Factor – user-entered rate scaling factor.
• Rate - calculated rate or slope between selected time points.
• R Squared –an indication of how well the rate vector fits the data points.
Working with Rate Vectors:
• To remove a particular rate vector, highlight the line and choose Remove in the rate vector box at the top
right of the pane.
• To move a rate vector, drag it to the desired location on the data curve.
• To change the start or end times associated with a vector- click either end and resize the vector to reflect
the desired time points. Alternatively, highlight the time in the rate table and overtype with the specific time
point of interest.
• To change the attributes of a rate vector, right-click it to open the following menu:
- Color - changes the color of a rate vector.
- Line Thickness - changes the thickness of a rate vector.
- Lock to Curve - determines whether a curve fit is applied to a rate vector. De-select this option to
manually move the vector.
- Delete - removes a rate vector.
Making Kinetic Measurements
1. Select a kinetics method from the main menu custom method list box. Alternatively, choose Measure from
within the Kinetics Editor application.
2. Select the cuvette pathlength. The default selection is 10 mm.
3. Select Heat to 37°C if appropriate. Allow the cuvette holder to reach temperature before proceeding. When
the Heat to 37°C box is selected, the current cuvette holder temperature will be displayed in the bottom
status bar.
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