User manual

Section 5-Kinetics
Data Acquisition Page
There are two graphical displays in the right pane of the Kinetics screen:
• The Wavelength table to the right of the display shows the absorbance values at each stage of the
measurement for all of the wavelengths being monitored.
- The spectrum plot at the bottom of the display shows a trace for each measurement taken in each stage.
The data is displayed with absorbance as the vertical axis and wavelength as the horizontal axis. Each
spectrum in the display is color-coded. The time and date each spectrum was collected is shown in the
upper-left corner of the spectrum plot area using the same color as the associated spectrum. The colored
vertical-line crosshairs correspond to the wavelengths the method was initially set to monitor. They can be
repositioned, or new ones added, to analyze other wavelengths of interest.
• The top Rate display allows rate vectors to be calculated for selected portions of associated data curves. A
data curve is the series of absorbance values at the wavelength selected in the Spectrum plot area at each
stage of the measurement. The data is shown as absorbance versus time where absorbance is the vertical
axis and time is the horizontal axis. Right-click on a data series to copy the data to a clipboard.
Calculating a Rate
1. If the wavelength of interest is not indicated by a cursor in the bottom display add the wavelength by
positioning the vertical crosshair to the desired wavelength and choosing Add.
- Up to 6 individual moveable cursors may be monitored and displayed.
2. Position the mouse over the initial time point of interest in the top wavelength display. Click and drag the
mouse to draw a line to the point at the end time of interest. The vector color will correspond to the primary
color associated with that wavelength cursor in the bottom panel although the associated wavelength ID in
the top display will be colored red for the active wavelength.
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