User manual

Section 5-Kinetics
Right Pane
When Kinetics Editor is selected, clicking on the icon at the top left will start a new method. A two-step wizard style
interface will appear at the bottom of the right pane. This wizard style interface includes Previous, Next, Finish and
Cancel buttons on the bottom right of the page.
The right pane features include:
• Kinetics Tab - displays the current method name, description, result label and time units. The default
setting for time units is seconds.
Two screen panels are used to define a method:
• Measure Stage – defines measurement stage intervals and the duration of each stage. To add the first
stage, enter a value in the highlighted Stage Interval field. Next, click on and enter a value in the highlighted
Stage Duration field. Click Enter and cumulative time will auto-populate. The next row will now be available
for additional stage entries. The minimum stage interval allowed is 2.0 seconds.
• Wavelengths to Monitor - monitors multiple wavelengths during the reaction. To add the first wavelength,
highlight the empty field and enter a value. The next row will become available for additional wavelength
selections.
- A cursor (colored vertical line) will be positioned at each of the monitored wavelengths. These cursors may
be moved and the associated changes will be reflected in the rate display.
- Additional wavelengths may be selected post measurement by positioning the black crosshair cursor at
the wavelength of interest and clicking Add.
- Wavelengths may be removed by selecting the associated vertical-line cursor and Remove.
- A trace for each specified wavelength will be added to the top panel once the measurement has been
initiated.
• Instrument settings - selects the spectral display wavelength range: UV, Visible, UV-Vis or custom.
A Kinetics method can either be saved or immediately run without saving. To save a Kinetics method, click Save on
the upper left side of the left pane.
To start the kinetics measurement, click on Measure on the upper left side of the left pane.
Note: When a measurement is initiated without first saving the method, the method settings will not be available
for review or re-use. The method would have to be recreated for another sample to be run under the same
conditions. Only one sample may be saved in each kinetic workbook. Kinetic data is not saved in autosave
files.
A previously saved Kinetics method can be edited by highlighting the method name within the method list box on the
"Home" screen and clicking Kinetics Editor. A previously saved Kinetics method can run by double-clicking on the
method name within the method list box. Alternatively a button can be added to the "Home" screen for the method
using the Option page.
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