User manual

Section 4- Method Editor
Example: Lysozyme (A(280)* 10 / 26.4) / Path() mg/ml
The above example will result in a “Lysozyme” column to be added to the report. The value will be the absorbance
at 280 nm multiplied by an adjustment factor of 10 divided by the constant 26.4 divided by the pathlength used in
centimeters.
Note: The 10 fold factor is used to adjust the E1% extinction coefficient value to a 0.1% value. This adjustment
allows the protein concentration to be reported in units of mg/ml.
Pedestal measurements will use the value 0.1 for the pathlength whereas cuvette measurements will apply the
user-selected pathlength.
All equation functions are case sensitive. Spaces are not allowed between a function name and “(.” For example-
A(280) is correct, but A (280) is incorrect.
Min(x, y) Minimum of x and y
Max(x, y) Maximum of x and y
abs(x) Absolute value of x
Ceiling(x) Round the fractional value of x up to an integer
Floor(x) Round the fractional value of x down to an integer
Round(x) Round decimal x to the nearest integer
Sqrt(x) Square root of x
Area(x, y) Area under the curve from wavelength x to y
Path() Represents the pathlength in cm used to calculate concentration
A(x) Absorbance at wavelength x
R(x) Reflectance at wavelength x
T(x) Transmittance at wavelength x
C(x) Concentration of specified component x
Ln(x) Natural logarithm of x
exp(x) Returns e raised to the power of x where e is 2.718
Log(x) or log10(x) Log base 10 of x
Pow(x, y) Take x to the y power
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