User manual

Section 4- Method Editor
Left Pane
When the Method Editor task bar is selected, the following icons will appear at the top of the left pane:
• New - creates a new custom method.
•
Save - saves a new custom method. This icon will be grayed out until the minimum setting requirements for
a new method have been entered. A new custom method may be saved to Groups other than Classic by
using the Copy to another group icon located under the method list drop down box.
• Measure - opens a new custom method once the method is saved to at least one group. This icon will be
grayed out until the minimum setting requirements for a new method have been entered.
• Delete - deletes the highlighted method in the method list box from the selected group.
Under the icons are two drop-down list boxes:
• Group List – selects the group to which the new methods is saved. The default group is Classic.
• Method List - displays the available methods associated with the selected group.
Right Pane
• Name & Type - defines the custom method name and type. The type refers to the method of calculating the
sample concentration and offers a choice of multiplying absorbance values by factors/extinction coefficients
or utilizing standard curves.
Method types include:
- Manually entered factor/extinction coefficient - a molecular weight is required if the results units and
the user defined factor/extinction coefficient units are not both weight based and molarity based. Click
Information icons within the Method Editor for additional information.
- Standard Curve - uses a simple standard curve based on the absorbance of a set of standards at the
specified analysis wavelength to determine a sample concentration.
- Single Point Standard Curve - uses the sample absorbance value divided by the average absorbance
value of the single standard to determine the sample concentration as a ratio of the standard
concentration.
- Standard Curve with two wavelengths - calculates the sample concentration by taking the average of
values determined by each of two standard curves associated with the user defined wavelengths.
- Advanced Standard Curve - calculates the sample concentration as determined by a standard curve
generated as a result of a user defined equation.
• Measurement - defines the analysis wavelength and the preferred result units. The measurement results or
concentration will be calculated using the absorbance value at the analysis wavelength and by applying the
selected method type (factor or standard curve). The default label for the result name is Concentration. If an
alternate user-defined label is entered, it will be utilized in the user-defined workbook and autosave files.
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The spectral display shows data for the current sample normalized to a 1 mm pathlength if measured
using the pedestal mode or the selected pathlength if using the cuvette option (model 2000c only). The
pathlength is indicated on the Y axis.
• Correction - selects both an across the spectrum baseline correction and a choice of three corrections for
the entered analysis wavelength. The analysis corrections of None, Single point, or Sloping baseline
subtract from the absorbance of the analysis wavelength. The baseline correction offsets the spectrum
such that the absorbance at the correction wavelength is subtracted from the entire spectrum.
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