User manual

Section 1-Introduction
Cuvette Measurements
The NanoDrop 2000c will accept 10 mm cuvettes up to 48 mm tall. When measuring samples using micro, semi-
micro, or ultra-micro cuvettes, we recommend using masked cuvettes. Masked cuvettes ensure that all light hitting
the detector has passed through the sample. Unmasked cuvettes can allow light to hit the detector that has not
passed through the sample. Expected differences between unmasked plastic cuvettes can lead to significant
measurement error, especially at low concentrations.
Un-masked Eppendorf Uvette® Starna masked micro cuvette
When measuring samples with analytical wavelengths in the UV (<340 nm), use cuvettes made of quartz, as these
pass UV wavelengths. Although some manufacturers offer "UV-transparent" plastic disposable cuvettes, even the
best of these are not transparent at wavelengths below 220 nm; most plastic and glass cuvettes block UV
wavelengths entirely.
While matched cuvettes have been recommended for single-beam instruments, many cuvette manufacturers have
sufficient controls over their manufacturing process to deliver excellent cuvette-to-cuvette performance without
requiring matching or certification. These cuvettes should be sufficient for measurements with the NanoDrop
2000c.
Cuvette Sample Size Requirements
It is essential to ensure that the sample volume in the cuvette is adequate to allow the light to pass through a
representative portion of the sample when making a measurement. The optical beam (2 mm) is directed 8.5 mm
above the bottom of the cuvette. Refer to the cuvette manufacturer for volume recommendations.
Cuvette Basic Use
1. Add the sample to the cuvette and ensure that the volume is sufficient to cover the light path.
2. Raise the sampling arm and insert the cuvette into the instrument. Insert the cuvette noting the direction of
the light path indicated by the etched arrow.
3. The sampling arm must be lowered into the down position for cuvette measurements.
4. Initiate a spectral measurement using the software on the PC.
5. When the measurement is complete, remove the cuvette, rinse thoroughly and dry between samples.
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