User manual

Section 3- Applications
4. Establish a blank using the appropriate buffer. The reference or blank solution generally is the buffer that
the molecule of interest is suspended or dissolved in. This solution should be the same pH and of a similar
ionic strength as the sample solution.
- Pedestal Option: Pipette 2 µL of the appropriate blanking solution onto the bottom pedestal and click
Blank.
- Cuvette Option (Model 2000c only): Insert the cuvette noting the direction of the light path indicated by
the etched arrow. The optical beam (2 mm) is directed 8.5 mm above the bottom of the cuvette. Refer to
the cuvette manufacturer for volume recommendations.
Note: The arm must be down for all measurements, including those made with cuvettes. It is
recommended that cuvettes be removed from the instrument prior to making a pedestal
measurement to ensure that the pedestal arm can move to the proper starting position
5. Enter a Sample ID in the appropriate field, load the first sample as described for the blank above and click
Measure.
Note: It is very important to ensure that cells have not settled prior to taking an aliquot for
measurement. A fresh aliquot of sample should be used for each measurement.
After the measurement:
- Simply wipe the upper and lower pedestals using a dry laboratory wipe and the instrument is ready to
measure the next sample.
- When using the cuvette option, remove the cuvette, rinse thoroughly and dry between samples.
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