User manual

Section 3- Applications
Unique Screen Features
The right pane displays features specific to the Cell Cultures application. Task bars in the left pane not described
below are described in “
Software Overview.”
The spectral display shows data for the current sample normalized to a 1 mm path if measured using the pedestal
mode or the selected pathlength if using the cuvette option (model 2000c only). The pathlength information is
indicated on the Y axis.
• Absorbance correction - user defined baseline cursor value. The baseline may be moved by selecting the
cursor and vertically dragging it to a different position or by entering a specific value.
• 600 nm (Abs) – 600 nm absorbance value, as measured from the user defined baseline.
• User cursor (nm) - user defined cursor position. The cursor may be moved by selecting it and horizontally
dragging to a different position or by entering the desired wavelength.
• User cursor (Abs) - absorbance value at the user defined wavelength, as measured from the user defined
baseline.
Making Cell Cultures Measurements
1. Select the Cell Cultures application from the main menu. If the wavelength verification window appears,
ensure the arm is down and click OK.
2. Select Add to report to automatically include all measurements in the current report. The default setting is
for all samples to be added to reports. The Add to report checkbox must be selected prior to a
measurement to save the sample data to a workbook.
3. Select Overlay spectra to display multiple spectra at a time.
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