User manual

Section 3- Applications
- Select the file drop-down option Use current settings as default as a convenient way to limit set-up time
for each new workbook.
4. Select Overlay spectra to display multiple spectra at a time.
5. Establish a blank using the appropriate buffer. It is advisable to use the dye reagent and protein buffer
without any protein added as both the blank and zero reference sample.
- Pedestal Option: Pipette 2 µL of blank solution onto the bottom pedestal, lower the arm and click Blank.
- Cuvette Option (Model 2000c only): Insert the cuvette noting the direction of the light path indicated by the
etched arrow. The optical beam (2 mm) is directed 8.5 mm above the bottom of the cuvette. Refer to the
cuvette manufacturer for volume recommendations.
Note: The arm must be down for all measurements, including those made with cuvettes. It is
recommended that cuvettes be removed from the instrument prior to making a pedestal
measurement to ensure that the pedestal arm can move to the proper starting position.
6. Under the Standards tab, highlight a standard and load as described for the blank above. Click Measure to
initiate the measurement. Be sure to measure all standards prior to measuring samples.
7. After all of the Standards have been measured, click on the Samples radio button. Enter a sample ID. Load
2 µL of sample when using the pedestal or insert the cuvette. Click Measure to initiate the measurement. It
is not necessary to blank the instrument between the standard and the unknown sample measurements.
Note: A fresh aliquot of sample should be used for each measurement.
After the measurement:
- Simply wipe the upper and lower pedestals using a dry laboratory wipe and the instrument is ready to
measure the next sample.
- When using the cuvette option, remove the cuvette, rinse thoroughly and dry between samples.
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