User manual

Section 3- Applications
Unique Screen Features
The right pane displays features specific to the Protein Pierce 660 nm application. Task bars in the left pane not
described below are described in “
Software Overview.”
The spectral display shows data for the current sample normalized to a 1 mm path if measured using the pedestal
mode or the pathlength selected if using the cuvette option (model 2000c only). The pathlength information is
indicated on the Y axis.
• Data tab - displays current measurement data.
• Standard Curve tab - displays the plot of the standard curve, the R
2
value and the type of curve being
displayed. Curve types include interpolation, linear and polynomials. The default selection is “linear.”
• Valid / Invalid Curve –indicates whether the minimum number of standards has been measured. Once the
criterion has been met, the standard curve status will change from Invalid Curve (red) to Valid Curve
(green).
Note: The status is only an indicator that the minimum number of required points has been established
for the curve fit type selected. The Standard Curve Status indicator does not validate the integrity
of the curve. Additional standards may be required to cover expected assay concentration range.
• Samples radio button – Once the criterion has been met for a valid curve, the Sample ID field will become
active when selected. The sample ID should be entered prior to making the measurement. The sample ID
should be entered prior to making the measurement.
• Standards radio button – enables entry of standard names and concentrations into the standards table
when selected.
• Concentration µg/ml - concentration of current sample in µg/ml.
•
Absorbance at 660 nm – absorbance of the Coomassie dye complex at 660 nm for the 1 mm pathlength.
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