User manual

Section 3- Applications
• Standards radio button - enables entry of standard names and concentrations into the standards table
when selected.
• Concentration mg/mL - concentration of current sample in mg/mL.
• Absorbance at 562 nm - the Cu-BCA complex’s absorbance at 562 nm.
BCA Standard Curves
A standard curve is required for BCA assays.
Example Standard Curve: 20:1 reagent/sample volume
• A standard curve can be generated using a minimum of two points which may include two standards or a
reference (BCA reagent/assay buffer only – no protein) and one standard.
• The multi-point curve capability allows for multiple replicates for up to seven standards. There is no set
order in which standards must be run.
• Additional standards may only be added before unknown samples are measured. Once the first sample is
measured, no additional standards or replicates may be added.
• A standard may be deleted at any time before a sample has been measured. Right-click the results table to
the far right to delete a standard or clear the entire standards table. Right-click a standard within the
replicates table under the graph display to delete individual standard replicates.
• Deleted standards cannot be added back or re-measured once the first sample is measured.
• In order to establish a new standard curve, a new workbook must be created. Colorimetric applications will
offer several options regarding the use of previous standard curves when appending sample data to
previous workbooks. It is recommended that the user follow the protein assay manufacturer’s guidelines for
generating standard curves before appending new data to a workbook.
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