User manual

Section 3- Applications
Pedestal Reconditioning
Solutions and reagents containing surfactants may “un-condition” the measurement pedestal surfaces so that the
liquid column does not form. If this occurs, use the NanoDrop Pedestal Reconditioning Compound (PR-1) as a rapid
means of reconditioning the pedestals when the surface properties have been compromised and liquid columns
break during measurement. Additional information about PR-1 may be found on our
website.
Unique Screen Features
The right pane displays features specific to the BCA Protein application. Task bars in the left pane not described
below are described in “
Software Overview.”
The spectral display shows data for the current sample normalized to a 1 mm path if measured using the pedestal
mode or the pathlength selected if using the cuvette option (model 2000c only). The pathlength information is
indicated on the Y axis.
• Data tab - displays current measurement data.
• Standard Curve tab - displays the plot of the standard curve, the R
2
value and the type of curve being
displayed. Curve types include interpolation, linear and polynomials. The default selection is “linear.”
• Valid / Invalid Curve - indicates whether the minimum number of standards has been measured. Once the
criteria have been met, the standard curve status will change from Invalid Curve (red) to Valid Curve
(green).
Note: The status is only an indicator that the minimum number of required points has been
established for the curve fit type selected. The Standard Curve Status indicator does not
validate the integrity of the curve. Additional standards may be required to cover expected
assay concentration range.
• Samples radio button - Once the criterion has been met for a valid curve, the Sample ID field will become
active when selected. The sample ID should be entered prior to making the measurement.
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