User manual

Section 3- Applications
Unique Screen Features
The right pane displays features specific to the Protein & Labels application. Task bars in the left pane not
described below are described in “
Software Overview.”
The spectral display shows data for the current sample normalized to a 10 mm path for all measurements including
measurements made with any cuvette pathlength (model 2000c only).
The following features are to the right of the spectral display:
• Sample ID - field into which sample ID is entered. The appropriate sample ID should be entered prior to
each measurement.
• Type - six sample types (options) are available for purified protein analysis and concentration
measurement. All of the options can be viewed by selecting the drop down menu adjacent to the box. The
sample type (color-keyed) is selected by using the mouse to highlight the preferred option. A complete
description of each sample type is provided within the Protein A280 application. The default setting is 1 Abs
= 1 mg/mL.
• Ext. Coeff, E1% L/gm-cm - appears only when the Other Protein (E1%) sample type is selected. The
appropriate extinction coefficient should be entered prior to making a measurement.
•
ε /1000 and M.W. (KDa) - appears when Other protein (E & MW) is selected. The appropriate e/1000 and
M.W. (kDa) should be entered prior to making a measurement.
• Conc - based on absorbance at 280 nm and the selected extinction coefficient. Concentration units may be
selected from the adjacent drop down list. Default is mg/mL.
• Dye 1 (or 2) - user selected dye(s). The defaults are Cy3 for Dye 1 and Cy5 for Dye 2. Abs. is the
normalized absorbance of the respective dye at the 1 mm pathlength.
- Abs- the normalized absorbance of the respective dye at the 1 mm pathlength.
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