User manual

Unique Screen Features .................................................................................................................................................. 3-30
Making Lowry Assay Measurements.............................................................................................................................. 3-32
P ROTEIN B RADFORD ........................................................................................................................................................... 3-34
Overview......................................................................................................................................................................... 3-34
Unique Screen Features .................................................................................................................................................. 3-35
Making Bradford Assay Measurements.......................................................................................................................... 3-37
P ROTEIN P IERCE 660 nm ..................................................................................................................................................... 3-39
Overview......................................................................................................................................................................... 3-39
Unique Screen Features .................................................................................................................................................. 3-40
Making Protein Pierce 660 nm Assay Measurements..................................................................................................... 3-42
C ELL C ULTURES .................................................................................................................................................................. 3-44
Overview......................................................................................................................................................................... 3-44
Unique Screen Features .................................................................................................................................................. 3-45
Making Cell Cultures Measurements.............................................................................................................................. 3-45
A PPLICATION FAQ S ............................................................................................................................................................ 3-47
4. METHOD EDITOR ............................................................................................................................................................. 4-1
E DITOR O PTIONS ................................................................................................................................................................... 4-1
M ETHOD E DITOR F EATURES ................................................................................................................................................. 4-1
Left Pane........................................................................................................................................................................... 4-2
Right Pane......................................................................................................................................................................... 4-2
M AKING C USTOM M ETHOD M EASUREMENTS ....................................................................................................................... 4-5
5. KINETICS............................................................................................................................................................................ 5-1
S CREEN F EATURES ................................................................................................................................................................ 5-1
Data Acquisition Page....................................................................................................................................................... 5-3
C ALCULATING A R ATE .......................................................................................................................................................... 5-3
M AKING K INETIC M EASUREMENTS ....................................................................................................................................... 5-4
6. WARRANTY AND MAINTENANCE ............................................................................................................................... 6-1
W ARRANTY ........................................................................................................................................................................... 6-1
Replacement Parts............................................................................................................................................................. 6-1
S AFETY .................................................................................................................................................................................. 6-1
WEEE C OMPLIANCE ............................................................................................................................................................. 6-1
M AINTENANCE OF THE S AMPLE R ETENTION S YSTEM ........................................................................................................... 6-2
Cleaning............................................................................................................................................................................ 6-2
Pedestal Reconditioning.................................................................................................................................................... 6-3
M AINTENANCE OF THE C UVETTE S YSTEM ............................................................................................................................. 6-4
M AINTENANCE FAQ S ........................................................................................................................................................... 6-5
7. DIAGNOSTICS AND TROUBLESHOOTING ..................................................................................................................7-1
I NTENSITY C HECK ................................................................................................................................................................. 7-1
C ALIBRATION C HECK ............................................................................................................................................................ 7-2
Pedestal Calibration Check Procedure.............................................................................................................................. 7-2
Cuvette Calibration Check................................................................................................................................................ 7-3
H ARDWARE ........................................................................................................................................................................... 7-3
Connection Errors ............................................................................................................................................................. 7-3
Power Restart.................................................................................................................................................................... 7-3
S AMPLE R ELATED I SSUES ...................................................................................................................................................... 7-5
Sample Measurement Accuracy and Reproducibility ....................................................................................................... 7-5
C ONTACT T ECHNICAL S UPPORT ............................................................................................................................................ 7-7
T ROUBLESHOOTING FAQ S .................................................................................................................................................... 7-9
8. APPENDICES...................................................................................................................................................................... 8-1
Q UICK S TART G UIDE ............................................................................................................................................................. 8-1
S OFTWARE F EATURE O VERVIEW ........................................................................................................................................... 8-1
C LEANING AND R ECONDITIONING G UIDE .............................................................................................................................. 8-1