User manual

Section 3- Applications
Unique Screen Features
The right pane displays features specific to the Protein A280 application. Task bars in the left pane not described
below are described in “
Software Overview.”
The spectral display shows data for the current sample normalized to a 10 mm path for all measurements including
measurements made with any cuvette pathlength (2000c only).
The following features are to the right of the spectral display:
• Sample ID - field into which a sample ID is entered. The appropriate sample ID should be entered prior to
each measurement.
• Type - six sample types (options) are available for purified protein analysis and concentration
measurements. The options can be viewed by selecting the drop down list adjacent to the Type box. The
sample type (color-keyed) is selected by highlighting the preferred option in the drop down list. A
description of each sample type is provided below. Default is 1 Abs = 1 mg/mL
A general reference setting based on a 0.1% (1 mg/mL) protein solution producing an
Absorbance at 280 nm of 1.0 A (where the pathlength is 10 mm or 1 cm).
Bovine Serum Albumin reference. Unknown (sample) protein concentrations are calculated
using the mass extinction coefficient of 6.7 at 280 nm for a 1% (10 mg/mL) BSA solution.
IgG reference. Unknown (sample) protein concentrations are calculated using the mass
extinction coefficient of 13.7 at 280 nm for a 1% (10 mg/mL) IgG solution.
Lysozyme reference. Unknown (sample) protein concentrations are calculated using the
mass extinction coefficient of 26.4 at 280 nm for a 1% (10 mg/mL) Lysozyme solution.
User-entered values for molar extinction coefficient (M-1 cm-1) and molecular weight (MW) in
kilo Daltons (kDa) for their respective protein reference.
User-entered mass extinction coefficient (L gm-1cm-1) for a 10 mg/mL (1%) solution of the
respective reference protein.
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