User manual

Section 3- Applications
UV-Vis
Overview
The UV-Vis application allows the NanoDrop 2000/2000c to function as a conventional spectrophotometer. Sample
absorbance is displayed on the screen from 190 nm to 840 nm. Up to 40 wavelengths can be designated for
absorbance monitoring and inclusion in the report.
Measurement Concentration Ranges
The NanoDrop 2000/2000c can measure samples with 10 mm pathlength absorbance equivalent of 300 A when
using the auto pathlength feature.
Unique Screen Features
The right pane displays features specific to the UV-Vis application. Task bars in the left pane not described below
are described in
‘Software Overview.”
The spectral display shows data for the current sample normalized to a 1 mm path if measured using the pedestal
mode or the selected pathlength if using the cuvette mode (model 2000c only). The pathlength information is
indicated on the Y axis.
The following features are to the right of the spectral display:
• Sample ID - field into which a sample ID is entered. The appropriate sample ID should be entered prior to
each measurement.
• Auto Pathlength – automatically allows the software to use the optimal pedestal pathlength for high
concentration samples. If selected, the shorter pathlength will be utilized when any wavelength between
220 nm to 840 nm has 1 mm equivalent absorbance value of 1.25 or higher. For wavelengths between 190
nm to 219 nm the change to the shorter pathlength occurs when any wavelength in this range has a 1 mm
equivalent absorbance value of 1.0 or higher.
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