User manual

Section 3- Applications
Micro Array
Overview
The capability to pre-select viable fluorescently-tagged hybridization probes for use on micro arrays can eliminate
potentially flawed samples and improve research effectiveness. The NanoDrop 2000/2000c measures the
absorbance of the fluorescent dye, allowing detection at dye concentrations as low as 0.2 picomole per microliter.
The software automatically utilizes the optimal pathlength to measure the absorbance of each sample.
Measurement Concentration Ranges
The NanoDrop 2000/2000c will accurately measure fluorescent-dye and nucleic acid concentrations up to 100
pmol/µl (Cy3) and 750 ng/µl (DNA) respectively without dilution. Refer to
“Measurement Ranges” for additional
information.
Dye/Chromophore Editor
The NanoDrop 2000/2000c software allows the user to either select from a list of pre-defined dyes or enter new
dyes using the Dye/Chromophore Editor. To enter a new dye, select the Show column (the first row). This will
activate the fields for manual entry of the appropriate information. Refer to the dye manufacturer for the appropriate
correction factors for user entered dyes. The 260 nm corrections will be automatically utilized for nucleic acid
sample concentration calculations when the respective dye is selected. Once entered, the information is saved.
To delete a user defined dye, highlight the row of interest by clicking in the grey box to the left of the (+) icon and
either use the keyboard delete key or right-click to bring up the delete option. Pre-defined dyes, identified by the
lock icon adjacent to the dye entry in the editor, may not be edited or deleted. The default settings are Cy3 for Dye 1
and Cy5 for Dye 2.
Unique Screen Features
The right pane displays features specific to the Micro Array application. Task bars in the left pane not described
below are described in “
Software Overview.”
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