User manual
Section 3- Applications
A Quick Start Guide that includes a blanking procedure can be accessed from “Appendices” of the NanoDrop 2000
User Manual PDF. It is recommended that this guide be printed and posted near the instrument as a reference.
Measurement Ranges
Sample Type Lower Detection Limit
A
pproximate
Upper Detection Limit
Typical Reproducibility
(5 replicates, SD = ng/uL; CV = %)
2 ng/µL (pedestal)
2 - 100 ng/uL:
+ 2 ng/uL
Nucleic Acids
0.4 ng/µL (cuvette)
< 15,000 ng/ µL (dsDNA)
>100 ng/uL:
+ 2%
2 ng/ µL (pedestal)
2 - 100 ng/uL:
+ 2 ng/uL
Microarray
0.4 ng/ µL (cuvette)
750 ng/ µL (dsDNA)
>100 ng/uL:
+ 2%
0.10 mg/mL (purified BSA -
pedestal)
Protein A280
0.010 mg/mL (purified BSA-
cuvette )
400 mg/mL (purified BSA)
0.10 - 10 mg/mL:
+ 0.10mg/mL >10
mg/mL:
+ 2%
0.10 mg/mL
(purified BSA - pedestal)
0.010 mg/mL
Proteins & Labels
(purified BSA- cuvette )
20 mg/mL (purified BSA) 0.10 - 10mg/mL: + 0.10mg/mL
0.2 mg/mL
(20:1 reagent.sample volume)
8.0 mg/mL
2% over entire range
.01 mg/mL 0.01 mg/mL over entire range
BCA
(1:1 reagent.sample volume)
0.20 mg/mL
Modified Lowry
0.2 mg/mL 4.0 mg/mL
2% over entire range
100 ug/mL 100-500 ug/mL: + 25ug/mL
Bradford
(50:1 reagent.sample volume)
8000 ug/mL
500-8000 ug/mL:
+ 5%
15 ug/mL 15 - 50 ug/mL: + 4 ug/mL
(1:1 reagent.sample volume) 100 ug/uL 50 - 125 ug/mL:
+ 5%
50 ug/ml
(15:1 reagent.sample volume)
50-125 ug/ml
> 125 ug/ml
50-125 ug/mL:
+ 3 ug/ml
>125 ug/mL: 2%
Pierce 660 nm
25 ug/ml
(7.5:1 reagent.sample volume)
25-125 ug/ml
> 125 ug/ml
25-125 ug/mL:
+ 3 ug/ml
>125 ug/mL: 2%
sample range 0.20-4.0 pmol/ul:
± 0.20 pmol/ul
Cy3, Cy3.5, Alexa
Fluor 555 and
Alexa Fluor 660
0.2 100
sample range >4.0 pmol/ul: ± 2%
sample range 0.12-2.4 pmol/ul:
± 0.12 pmol/ul
Cy5, Cy5.5 and
Alexa Fluor 647
0.12 60
sample range >2.4 pmol/ul: ± 2%
sample range 0.40-8.0 pmol/ul:
± 0.40 pmol/ul
Alexa Fluor 488
and Alexa Fluor
594
0.4 215
sample range >8.0 pmol/ul: ± 2%
sample range 0.30-6.0 pmol/ul:
± 0.30 pmol/ul
Alexa Fluor 546 0.3 145
sample range >6.0 pmol/ul: ± 2%
Note: Measurement ranges noted for dyes in the above are specific to pedestal measurements.
3-2