User manual

Section 2-Software
Right Pane
The right pane displays the main menu which includes the following:
• Group drop-down box - selects the preferred main menu display and associated applications. The default
selection is the Classic NanoDrop group of applications.
• Predefined application buttons - and additional main menu selections.
• User-Defined list - empty until user defined methods or kinetics methods are created. Once created,
quantification methods have a scale icon to their left while kinetics methods have a clock icon to their left.
Once an application is opened, the right pane displays the sample spectra as well as drop-downs and data fields
relevant to the specific application. A pedestal or a cuvette icon is displayed in the user guidance text box above the
graph. The icon shown indicates which mode will be used to make the next measurement.
Graph displays
The graph panel displays the most recent sample spectrum. When Overlay Spectra is selected, the data from
multiple samples is displayed. The sample ID is shown at the top left of the display panel. The most recent sample
measured will be at the top of the list and the text and associated spectrum are shown in red.
Note: Clicking a previously measured sample turns that sample ID text and associated spectrum red but does
not highlight that particular sample in the report view at the bottom of the page.
Although there is no practical limit to the number of sample spectra that can be displayed, the software can only
display approximately 28 sample IDs in the left hand legend when viewed in a full screen window. Once the display
limit is reached, the list becomes a drop down box as indicated by a black arrow icon at the top left of the display.
Use the arrow to display the previous sample IDs. Right-click in the graph display to bring up a menu box which
includes an option to turn off the sample legend.
A single region can be enlarged by simply drawing a box around the area of interest and then clicking inside the
box. Alternatively, a chevron at the bottom of the graph display opens up the view finder which displays a single
sample spectrum. The bold lines at the ends of the view finder are movable (click and drag) and define the
wavelengths to display on the graph.
To zoom back out, right-click the display to open a multi-option menu:
• Autoscale all samples - sets the upper limit of the Y-axis to the highest absorbance value for all of the
displayed spectra and the lower limit to the lowest absorbance value for all of the displayed spectra.
• Full Display all samples - resets the X and Y axis to display the full spectra for all samples.
• Set Scale - allows manual setting of minimum and maximum values for the Y axis.
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