User manual

Section 2-Software
- E-mail current workbook - automatically attaches the current workbook to a new email message.
When sending data to NanoDrop products technical support, please use the My Data task bar to locate
the appropriate autosave data file. Open the file with the NanoDrop 2000/2000c software and send to
Technical Support.
Note: Although all Report fields may not be displayed when opening an autosave file, all fields are
recoverable by the Technical Support team.
• Help - searchable help files may be accessed from any screen using the [Ctrl]-m, [Ctrl]-h or the F1 key.
Refer to “Preferences” under “
Data and Account Management” for more information about shortcut keys. A
PDF version of the NanoDrop 2000 User Manual is available under the Windows Start menu.
The About option under the Help menu provides information specific to the current software version and
instrument.
Left Pane Instrument Settings
• Add to Report - indicates whether sample data should be added to the current report. Although all data is
archived to an autosave workbook and can be retrieved at a later date, Add to Report should be selected
on a routine basis for easy data retrieval. Select Add to report prior to a measurement to save the sample to
a report and a workbook.
• Overlay spectra - displays multiple spectra. A crosshair cursor is displayed and linked to the most recent
sample measured. When Overlay Spectra is selected, click a spectrum to link the crosshairs to the
spectrum.
- The overlaid sample IDs are listed on the top left side of the graph display. The last sample measured
appears at the top of the legend and will be labeled red. Clicking on a different spectrum will cause the
corresponding sample ID to appear in red.
- Un-checking Overlay Spectra will clear the display of all spectra when a new measurement is made.
• Small sample volume – (Nucleic Acid and Protein A280 applications only) –allows measurements of just
0.5 µL when samples have 10 mm equivalent absorbance values of 3.0 or higher (e.g. > 150 ng/µL
dsDNA).
• Use Cuvette (NanoDrop 2000c model only) - enables cuvette measurements. Options available for use
with cuvette measurements include:
- Pathlength - includes pathlengths of 10, 5, 2, and 1 mm.
- Stir speed - speed settings range from 1-10. The default setting is Off.
- Heat to 37 °C - heats the cuvette holder to 37 ±0.5 °C. When selected, the current temperature of the
cuvette will be displayed at the bottom of the software screen. It may take between 1-10 minutes for the
cuvette holder to reach 37 °C. The time for the sample to reach 37 °C will depend on the starting
temperature of the liquid. The heater is turned off as the default setting.
Note: If moving to a different application, the heater will turn off. However, the heater will
continue to warm the cuvette block if pedestal measurements are made within the same
application.
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