Specifications

3. Defining Measurements
2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.8 63
Use Gain Regulation (available for all Infinite readers)
The command Use gain regulation is only available for plate-wise kinetic
measurements in fluorescence top/bottom and fluorescence polarization mode.
Upon activating Use gain regulation, fluorescence kinetic measurements with
increasing signals are prevented from running into “OVER” once the samples
produce too high RFU values. Instead the initially set gain (manual/ optimal/
calculated from well) is automatically reduced in order to permit the measurement
of even very high signals.
Results that are obtained with different gain settings are highlighted accordingly.
All RFU values with different gain settings are automatically correlated, allowing
the evaluation of the entire kinetic data within one and the same graph.
Kinetics: x% of Gain (available for all Infinite readers)
The function “x% of … gain” is available for plate-wise kinetic measurements in
Fluorescence Top/Bottom and Fluorescence Polarization mode.
The following options are available:
• Start a kinetic measurement with x% of “optimal” gain (optimal gain is
calculated in a pre-measurement based on the highest signal within the
defined well range on the microplate and set as initial gain for the kinetic
measurement)
• Start a kinetic measurement with x% of “calculated from well” gain (the
optimal gain setting for one defined well is calculated in a pre-measurement
and set as initial gain for the kinetic measurement)
The percentage of the initial gain may be set individually from 20-100%, with
100% being set as default value.