Specifications

2. Measurement Parameter Editor
2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.8 37
Z-Position Z-position (available for Infinite F500 and M1000)
The Z-position represents the height of the measurement
head above the microplate. It can be determined as follows:
Manual (default value: 20000 µm)
Calculated from well: the instrument automatically
calculates the optimal Z-position for to one selected well and
applies this value to all other wells within the selected well
range.
Same as: may be used for measurements with more than
one measurement label. The Z-position is equal to that of the
previous label.
Instrument / Z-position control: may be used to determine
the appropriate Z-position from a graphical scheme. The
resulting value is applied to all further measurements until a
different Z-position is entered.
For more detailed information on Z-positioning refer to the
Instructions for Use of the Infinite F500 or M1000 instrument.
Measurement
If the Measurement Blank range should be defined, click
Change.
G-Factor
The G-Factor compensates for differences in optical
components between the parallel and perpendicular
measurement.
The G-Factor is the correction factor that can be determined
for the wavelengths of the fluorophore by measuring a
sample with a known polarization value.
Uncalibrated G-Factor: If no calibrated G-factor is available,
the default value of 1 will be displayed and marked as
Uncalibrated G-Factor. In order to enable the
measurement, confirm this value or select a new one by
either clicking the up and down arrows or by entering a value
manually.
Calibrate: When selecting Calibrate, the G-factor is
determined for the current measurement parameters and
used for the following FP measurement. In order to perform
the G-Factor calibration, please define:
Reference value: Select a polarization value that shall be
used for reference e.g. 20 mP.
Reference range: Click on Change and select the wells
filled with the reference fluid, e.g. 1 nM fluorescein.
Blank range: Click on Change and select the wells filled
with the reference blank. Select Same as measurement
blank if the reference blank is the same as the measurement
blank.
For further details see the respective Instructions for Use of
the instrument connected.
Read Specify a certain Number of flashes and, if required a
Settle time before the measurement.