Specifications

2. Measurement Parameter Editor
36 Instructions for Use for i-control No. 30018668 Rev. No. 1.8 2011-03
Enter or select the respective parameters:
Wavelength
Filter instruments configured for Fluorescence Polarization
(FP) measurements are delivered with a standard FP filter
slide. The filter slide is equipped with filters and polarizers for
excitation and emission, at 485 and 535 nm respectively, and
can be applied, for example, for fluorescein-based FP
applications.
Bandwidth For the Infinite M1000 instrument, the emission bandwidth
can be entered.
Hide/Show
Details:
Integration
Integration time: duration of signal recording per well (valid
range: 20-2000 µs). For Infinite M1000 instruments the
integration time is defined by the number of flashes. 1 to
1000 flashes can be selected (1 flash is 10 ms integration
time).
Lag time: time between flash and the start of signal
integration.
While lag time is an optional function, the integration time is
a mandatory parameter determining the duration of signal
recording. The default values for standard fluorescence
intensity measurements are 0 µs lag time and 20 µs
integration time. TRF measurements typically require a lag
time according to the respective application.
Mirror Mirror (available for Infinite F500)
The availability of mirrors depends on the selected plate
format and on the types of dichroic mirrors that are installed.
According to the selected filter wavelengths the appropriate
mirror may be set by the instrument (selection “automatic”) or
manually. Custom dichroic mirrors may be installed and
defined by the user.
For further details on mirrors and mirror selection refer to the
Instructions for Use of the Infinite F500 instrument.