Specifications

2. Measurement Parameter Editor
2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.8 33
Hide/Show TRF
Settings:
Integration/Lag time
Integration time: duration of signal recording per
well (valid range: 20-2000 µs).
Lag time: time between flash and the start of signal
integration.
While lag time is an optional function, the integration
time is a mandatory parameter determining the
duration of signal recording. The default values for
standard fluorescence intensity measurements are
0 µs lag time and 20 µs integration time. TRF
measurements typically require a lag time according
to the respective application.
Gain
The gain is an amplification factor for the
photomultiplier tube (PMT) and may be set by
selecting one of the following modes:
Manual gain: user-defined gain value (valid range: 1-
255)
Calculated from well: determines the optimal gain
for the selected well. The resulting gain value is
applied to all other wells within the selected well
range.
Read Specify a certain Number of flashes and, if required,
a Settle time before the measurement.
Flashes When connected to an Infinite M1000 instrument,
select one of the following options and, optionally,
enter a Settle Time:
Mode 1 (400 Hz)
Mode 2 (100 Hz)
In order to obtain a good measurement precision it is
recommended to perform fluorescence
measurements with the number of flashes that is set
as a default value for the respective instrument.
Infinite M1000 allows switching between two flash
frequencies for the Fluorescence Intensity and
Fluorescence Intensity Scan mode: 100 or 400 Hz
(corresponding to 100 or 400 flashes per second,
respectively). The energy of one flash is app. 0.1
Joule for the 400 Hz mode and app. 0.4 Joule for the
100 Hz mode. For standard fluorescence
measurements it is recommended to use the 400 Hz
mode.
For TRF measurements the 100 Hz mode is
recommended to improve the results.
Label
Type in a label name.