Specifications

2. Measurement Parameter Editor
30 Instructions for Use for i-control No. 30018668 Rev. No. 1.8 2011-03
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TRF settings:
Integration/Lag
time
Integration time: duration of signal recording per well (valid
range: 20-2000 µs).
Lag time: time between flash and the start of signal
integration.
While lag time is an optional function, the integration time is
a mandatory parameter determining the duration of signal
recording. The default values for standard fluorescence
intensity measurements are 0 µs lag time and 20 µs
integration time. TRF measurements typically require a lag
time according to the respective application.
Mirror Mirror (available for Infinite F200 Pro and F500)
The availability of mirrors depends on the selected plate
format and on the types of dichroic mirrors that are installed.
The mirror selection for Infinite F500 looks as follows:
The mirror selection for Infinite F200 Pro is limited to the
options 50% mirror, Automatic and Dichroic 510 (e.g.
fluorescein).
According to the selected filter wavelengths the appropriate
mirror may be set by the instrument (selection “automatic”) or
manually. Custom dichroic mirrors may be installed and
defined by the user (Infinite F500 only).
For further details on mirrors and mirror selection refer to the
Instructions for Use of the Infinite F200 Pro and F500
instrument, respectively.
Z-Position Z-Position (available for Infinite M200 Pro, F500 and
M1000)
The Z-position represents the height of the measurement
head above the microplate. It can be determined as follows:
Manual (default value: 20000 µm)
Calculated from well: the instrument automatically
calculates the optimal Z-position for one selected well and
applies this value to all other wells within the selected well
range.
Same as: may be used for measurements with more than
one measurement label. The Z-position is equal to that of the
previous label.
Instrument / Z-position control: may be used to determine
the appropriate Z-position using a graphical scheme. The
resulting value is applied to all further measurements until a
different Z-position is entered. The Z-position control in the
Infinite M200 Pro and Infinite M1000 also allows for defining
two wells as Blank and Signal, respectively, and setting the
Z-position to the value that gives the best Signal-to-Blank
(S/B) ratio.
For more detailed information on Z-positioning refer to the
Instructions for Use of the Infinite M200 Pro, F500 and
M1000 instrument, respectively.