Specifications

2. Measurement Parameter Editor
2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.8 29
Bandwidth For the Infinite M1000 instrument, the bandwidth for
excitation and emission can be selected.
Read Specify a certain Number of flashes and, if required, Settle
time before the next measurement. The number of flashes is
selectable from 1 – 100 (up to 200 for M1000 only).
Settle time: Enter a value to specify the time before the start
of the measurement.
Flashes When connected to an Infinite M1000 instrument, select one
of the following options and, optionally, enter a Settle Time:
• On-the-fly
• Mode 1 (400 Hz)
• Mode 2 (100 Hz)
On-the-fly measurements with one flash per well are possible
with all plate types.
In order to obtain a good measurement precision it is
recommended to perform fluorescence measurements with
the number of flashes that is set as a default value for the
respective instrument.
Infinite M1000 allows switching between two flash
frequencies for the Fluorescence Intensity and Fluorescence
Intensity Scan mode: 100 or 400 Hz (corresponding to 100 or
400 flashes per second, respectively). The energy of one
flash is app. 0.1 Joule for the 400 Hz mode and app. 0.4
Joule for the 100 Hz mode. For standard fluorescence
measurements it is recommended to use the 400 Hz mode.
For TRF (time resolved fluorescence) measurements the 100
Hz mode is recommended to improve the results.
Mode Select Top or Bottom.
Label
Enter a label name.
Gain
The gain is an amplification factor for the photomultiplier tube
(PMT) and may be set by selecting one of the following
modes:
Manual gain: user-defined gain value (valid range: 1-255)
Optimal gain: calculated automatically by the instrument
according to the highest signal within the selected well range
in order to avoid OVER. Optimal gain determination is
performed in a pre-measurement. It is recommended to use
the optimal gain function for all applications that produce
results with unknown RFU values.
Calculated from well: determines the optimal gain for the
selected well. The resulting gain value is applied to all other
wells within the selected well range.
Extended dynamic range: (available for all Infinite readers)
The extended dynamic range option is an automatic gain
function that serves to optimally adjust the gain setting for
both very high and very low signals on a microplate within
one single measurement. By selecting “extended dynamic
range”, the measurement is done in two consecutive parts,
one with a high and one with a low gain. The results of both
measurements are automatically correlated and displayed
within one single data set.