Instructions for Use for i-control Document Part No.: 30018668 2011-03 Document Version No.: 1.8 Software Version No.: 1.
Warnings, Cautions, and Notes The following types of notices are used in this publication to highlight important information or to warn the user of a potentially dangerous situation: Note Gives helpful information. CAUTION INDICATES A POSSIBILITY OF INSTRUMENT DAMAGE OR DATA LOSS IF INSTRUCTIONS ARE NOT FOLLOWED. WARNING INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT FOLLOWED.
WARNING CAREFULLY READ AND FOLLOW THE INSTRUCTIONS PROVIDED IN THIS MANUAL BEFORE OPERATING THE INSTRUMENT. Notice Every effort has been made to avoid errors in text and diagrams; however, Tecan Austria GmbH assumes no responsibility for any errors which may appear in this publication. It is the policy of Tecan Austria GmbH to improve products as new techniques and components become available.
Tecan Customer Support Address Contact Tecan Asia Pte Ltd. 18 Boon Lay Way, #09-140 TradeHub 21 Singapore 609966, Singapore T +65 6444 1886 F +65 6444 1836 tecan@tecan.com.sg Tecan Australia Pty Ltd. 10-22 Hornibrook Esplanade Clontarf Qld 4019 Australia T 1300 808 403 (free in Australia) T +61 7 3105 5010 F +61 7 3283 7922 support@tecan.com.au Tecan Austria GmbH Untersbergstrasse 1a A-5082 Grödig Austria T +43 6246 8933 256 F +43 6246 72 770 helpdesk-at@tecan.
Table of Contents 1. Introduction .................................................................................. 7 1.1 1.2 1.3 1.4 Area of Application .......................................................................... 7 1.1.1 i-control Intended Use .............................................................. 7 Specifications .................................................................................. 7 1.2.1 Hardware Requirements .......................................................
4.2 4.1.6 Help Menu.............................................................................87 Toolbar ............................................................................................ 88 5. Batch Processing ....................................................................... 91 5.1 5.2 5.3 5.4 5.5 Introduction .................................................................................... 91 Microplate Requirements for Batch Processing ..........................
1. Introduction 1. Introduction 1.1 Area of Application i-control is an easy-to-use and flexible tool, which gives the user complete control over Tecan readers. i-control presents the raw data for further use in Excel, offering excellent features for research purposes. Note Depending on the instrument connected and the modules equipped, certain i-control features may be disabled or invisible. 1.1.
1. Introduction Memory Minimum Recommended Windows XP: 512 MB RAM 1 GB RAM Windows Vista (32-bit): 1 GB RAM 2 GB RAM Windows 7 (32-bit): 1 GB RAM Windows 7 (64-bit): 2 GB RAM Space Requirements 700 MB Monitor Super VGA Graphics Resolution 1024 x 768 Color Depth 256 Mouse Microsoft mouse or compatible pointing device Communication 1 x USB 2.0 Devices 1 x CD-ROM drive 2 GB RAM 3 GB RAM 1 GB 1280 x 1024 2 x USB 2.
1. Introduction 1.2.
1. Introduction 1.3 Software Installation CAUTION YOU MUST HAVE ADMINISTRATIVE RIGHTS TO INSTALL THE SOFTWARE. CAUTION INSTALL THE SOFTWARE BEFORE PLUGGING THE INSTRUMENT INTO THE COMPUTER. The i-control software is installed using the following procedure: 1. 2. 3. Insert the installation CD into the appropriate disk drive or CD ROM drive. Open the Windows Explorer and browse to folder Software on the installation CD. Double-click i-Control .exe to start the installation procedure.
1. Introduction 5 6. 1.3.1 In the course of the installation a series of dialog boxes will appear. Read each one, enter any necessary information and click Next to continue. The files are installed and the program icon is created. When the Installation Complete dialog box appears, click Finish and the i-control program is ready to be used.
1. Introduction If Click here for status is not clicked, several windows appear with information in appearing and fading balloons about the current status of the installation (this screenshot shows the last balloon, confirming successful installation of the software): 12 Instructions for Use for i-control No. 30018668 Rev. No. 1.
1. Introduction 1.3.2 Hardware Wizard (only valid for WindowsXP) If the instrument is plugged in after the software has been installed, the following Hardware Wizard dialog boxes appear: Depending on system configuration and installed drivers, this dialog box may appear first: Select No, not this time and click Next. Select Install the software automatically and click Next. 2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.
1. Introduction The Hardware Wizard searches for the device. After the device has been found, click Next. Depending on existing previous drivers, the following dialog box appears: The wizard suggests the appropriate device. Select Next to complete the New Hardware Wizard. 14 Instructions for Use for i-control No. 30018668 Rev. No. 1.
1. Introduction Click Finish to complete installation. The software is ready for use. 2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.
1. Introduction 1.4 Starting i-control i-control can be used either with a connected instrument or in simulation mode. 1.4.1 Connected Instrument CAUTION INSTALL THE SOFTWARE BEFORE CONNECTING THE INSTRUMENT TO THE COMPUTER. Connect the instrument to your computer and switch the instrument on. Start the program by selecting Programs/Tecan/i-control from the Windows Start menu.
1. Introduction Example for the Infinite M1000 instrument: In the Connect to: dialog box select the instrument name. Connect built-in stacker: With the Infinite M1000 instrument, only the built-in stacker can be used (see screenshot). Click OK to start i-control. 2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.
1. Introduction 1.4.2 Simulated Instrument Start the program by selecting Programs/Tecan/i-control from the Windows Start menu. In the Connect to Instrument dialog box, select Show simulated instruments; from the Instrument Name list, select the demo instrument to connect to. After selecting the simulated instrument, a drop-down list appears offering several options, depending on the instrument selected above.
1. Introduction For the Infinite M1000, for example, these options are: • M1000_384/1536 • M1000_FP_INJ_STACKER • M1000_384/1536 LCE • M1000_FP_INJ_STACKER LCE For the Infinite F50, for example, these options are: • F50PRO_4_FILTERS • F50PRO_8_FILTERS Connect built-in stacker: With the Infinite M1000, the built-in stacker can be simulated. See selections as shown in the screenshot above.
2. Measurement Parameter Editor 2. Measurement Parameter Editor 2.1 Introduction The main window of the i-control software is the Measurement Parameter Editor, which is used to set up workflows. Each workflow is easily created by dragging and dropping the process steps into a sequence according to the application. The application workflow is then visible to the user in the workflow pane and can be saved for future use.
2.
2. Measurement Parameter Editor Miscellaneous 2.2.1 Comment User Request Wait for Temperature Wait (Time) Incubation Lab Ware Plate The Plate program element is used to select a plate format from the Plate definition drop-down list. Click Details... to see further information on the selected plate. If a plate with cover is used, select the Plate with cover checkbox. The measurement will automatically measure all selected wells of the plate.
2. Measurement Parameter Editor Part of Plate The Part of Plate program element is displayed according to the selected plate format (number of wells). To measure individual wells, click the desired well or to measure a range of wells drag a frame around the desired range.
2. Measurement Parameter Editor Cuvette The Cuvette program element allows performing absorbance measurement in fixed wavelength and scan mode. This option is only available for the Infinite M200 and M200 Pro. 2.2.2 Measurements For detailed information on measurement methods, refer to the respective Instructions for Use of the instrument connected. Absorbance The Absorbance program element is used to perform absorbance measurements.
2. Measurement Parameter Editor Absorbance Scan The Absorbance Scan program element is available with the Infinite M200, M200 Pro and M1000. Enter or select the respective parameters: Wavelength From: The lower wavelength limit To: The upper wavelength limit Step: Enter a valid value. Read Number of flashes: Indicates the number of flashes (select a number between 0 – 100). Settle time: The time between movement of the plate and starting of the read (selectable from 0 – 1000 ms).
2. Measurement Parameter Editor Example when connected to an Infinite M200 Pro: Example when connected to an Infinite F200 Pro: 2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.
2. Measurement Parameter Editor When connected to an Infinite F500, this program element looks different: parameter fields for Mirror and Z-Position are added: When connected to an Infinite M1000, this program element looks different: parameter fields for Bandwidth, Show/Hide TRF settings and additional flash modes are available. The following are the Fluorescence Intensity parameters: Wavelength 28 Specify an Excitation and an Emission wavelength.
2. Measurement Parameter Editor Bandwidth For the Infinite M1000 instrument, the bandwidth for excitation and emission can be selected. Read Specify a certain Number of flashes and, if required, Settle time before the next measurement. The number of flashes is selectable from 1 – 100 (up to 200 for M1000 only). Settle time: Enter a value to specify the time before the start of the measurement.
2. Measurement Parameter Editor Hide/Show TRF settings: Integration/Lag time Integration time: duration of signal recording per well (valid range: 20-2000 µs). Lag time: time between flash and the start of signal integration. While lag time is an optional function, the integration time is a mandatory parameter determining the duration of signal recording. The default values for standard fluorescence intensity measurements are 0 µs lag time and 20 µs integration time.
2. Measurement Parameter Editor Fluorescence Intensity Scan The Fluorescence Intensity Scan program element is available with the Infinite M200, Infinite M 200Pro and the Infinite M1000. Example Infinite M200 Example Infinite M200 Pro 2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.
2. Measurement Parameter Editor Example Infinite M1000 Enter or select the respective parameters: 32 Scan Selection Select either Excitation Scan or Emission Scan. With the Infinite M1000 instrument, also the option 3D Scan can be selected. Excitation Wavelength Values can only be entered if Excitation Scan is selected. From: Specify the range of the excitation by entering a value. To: Specify the range of the excitation by entering a value. Step: Enter a valid value.
2. Measurement Parameter Editor 2011-03 Hide/Show TRF Settings: Integration/Lag time Integration time: duration of signal recording per well (valid range: 20-2000 µs). Lag time: time between flash and the start of signal integration. While lag time is an optional function, the integration time is a mandatory parameter determining the duration of signal recording. The default values for standard fluorescence intensity measurements are 0 µs lag time and 20 µs integration time.
2. Measurement Parameter Editor Fluorescence Polarization (available for F200, F200 Pro, F500 and M1000) The Fluorescence Polarization (FP) program element is used to measure the rotational mobility of a fluorescent compound. Fluorescence polarization measurements are based on the detection of the extent of depolarization of fluorescence emission light after excitation of a fluorescent molecule by polarized light.
2. Measurement Parameter Editor When connected to an Infinite F500, this program element looks different: parameter fields for Mirror, Z-Position and Plate-wise are added: Example when connected to an Infinite M1000 instrument: 2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.
2. Measurement Parameter Editor Enter or select the respective parameters: Wavelength Filter instruments configured for Fluorescence Polarization (FP) measurements are delivered with a standard FP filter slide. The filter slide is equipped with filters and polarizers for excitation and emission, at 485 and 535 nm respectively, and can be applied, for example, for fluorescein-based FP applications. Bandwidth For the Infinite M1000 instrument, the emission bandwidth can be entered.
2. Measurement Parameter Editor 2011-03 Z-Position Z-position (available for Infinite F500 and M1000) The Z-position represents the height of the measurement head above the microplate. It can be determined as follows: Manual (default value: 20000 µm) Calculated from well: the instrument automatically calculates the optimal Z-position for to one selected well and applies this value to all other wells within the selected well range.
2. Measurement Parameter Editor 38 Gain The gain is an amplification factor for the photomultiplier tube (PMT) and may be set by selecting one of the following modes: Manual gain: user-defined gain value (valid range: 1-255) Optimal gain: calculated automatically by the instrument according to the highest signal within the selected well range in order to avoid OVER. Optimal gain determination is performed in a pre-measurement.
2. Measurement Parameter Editor Multiple Reads per Well The i-control software allows the user to define multiple reads per well (MRW) in Absorbance, Fluorescence top and Fluorescence bottom mode. The MRW feature is not available for well wise measurements. The Reference wavelength on the absorbance program element is not selectable in combination with multiple reads per well. For Infinite M1000 instruments there is a minimum Settle time of 10 msec required as soon as Multiple reads per well is selected.
2. Measurement Parameter Editor Optimal Read Similar to the MRW read mode, the "Optimal Read" function is a measurement on multiple, spatially separated spots inside the well. The spots are arrayed to cover the whole well area in order to achieve maximal well illumination.
2. Measurement Parameter Editor Luminescence The Luminescence program element is used to determine the activity of a luminescent compound. Example for the Infinite 200 Example for the Infinite200 Pro Enter or select the respective parameters: Attenuation For strongly luminescent samples it may be necessary to apply neutral density filters to reduce the luminescent signal. Select the desired attenuation option. The options depend on the instrument connected: F/M200 F/M200 Pro F500 M1000 M1000 FW 2.
2. Measurement Parameter Editor Filters Use of Color Filters for Single Luminescence: (available for Infinite F500 and M1000) All filters that are available for dual color luminescence may be used in single luminescence measurements as well. Besides the attenuation functions an additional dropdown list in the attenuation field displays the filters for GREEN, GREEN1, BLUE and MAGENTA to be selected individually for single luminescence applications.
2. Measurement Parameter Editor 2.2.3 Wavelengths From: Select the starting wavelength for the scan. To: Select the endpoint wavelength for the scan. Step: enter a valid value Bandwidth: Select a value from the drop down list. Mode Select Top or Bottom. Gain Default: this value is instrument specific (see also 30036266_IFU_InfiniteM1000) Manual: User-defined gain value (valid range 1-255) Integration Integration time: enter a value to specify the duration of integration.
2. Measurement Parameter Editor Shaking Select the Shaking program element if the plate is to be shaken, either before the measurement or between kinetic cycles. Enter the respective parameters: Duration Enter the duration of the shaking process. Mode Select between the options Linear, Orbital and Double Orbital from the drop-down list. The Mode Double Orbital is available for Infinite M1000 for FW 2.0 and higher (Ref 30061442. Amplitude Enter the required Amplitude value from the drop-down list.
2. Measurement Parameter Editor Injection The Injection program element is dependent on a precedent well strip to inject liquid into one well after the other. See also 3.3.2 The Difference between “Inject” and “Dispense”. Example for the Infinite M200 Pro The following are the Injection parameters: Select Injector Select either Injector A or B if the instrument is equipped with two injectors. Volume: Specifies the volume to inject into a single well.
2. Measurement Parameter Editor Refill for every injection: Refilling of the syringe occurs for each injection step. Click the link Wait after injection to define the time for starting the next workflow. See 2.2.5 Miscellaneous - Wait (Timer). Dispense The Dispense program element is always used plate-wise to fill the plate (or part of plate) with liquid. See 3.3.2 The Difference between “Inject” and “Dispense”. Example for the Infinite 200 Pro 46 Instructions for Use for i-control No. 30018668 Rev. No.
2. Measurement Parameter Editor The following are the Dispense parameters: Select Injector Select either Injector A or B if the instrument is equipped with two injectors. Volume: Specifies the volume to inject into a single well. Speed: Specifies the speed of liquid flow while dispensing. Refill Speed equal to Dispense Speed: Clear the check box to enter the refill speed which may be different than the injection speed. The syringe can be filled faster, even if the dispensing speed is low.
2. Measurement Parameter Editor 2.2.4 Kinetic Kinetic Cycle Use the program element Kinetic Cycle to perform several consecutive measurements, which may be executed in certain intervals. Enter the respective parameters: Cycles Number of cycles: Enter a number or click the up or down arrows for the number of actual measurement steps (2 – 1000 cycles) Duration: Enter the duration, format hh:mm:ss. Kinetic Interval Use kinetic interval: Enter the time interval (hh:mm:ss or ms).
2. Measurement Parameter Editor After having started the measurement, it is possible to interrupt a plate-wise kinetic measurement clicking the Pause button and to continue: Kinetic Condition Use the Kinetic Condition program element to define which actions should be executed at a certain cycle. If 3 is entered for Execute command at cycle within a kinetic measurement containing, e.g. a Shake step, shaking is performed only at cycle 3.
2. Measurement Parameter Editor 2.2.5 Miscellaneous Comment Use the program element Comment to enter a remark or statement for the current measurement in the text field. This text is shown together with the measurement in the Excel output sheet. User Request The User Request program element informs the operator of the instrument to execute a definite action during the workflow at a certain time.
2. Measurement Parameter Editor Enter the respective parameters: Timer Enter the Wait time (hh:mm:ss) Options Wait for injection: The time for injection is included in the wait time. Ignore wait at last kinetic cycle: When the program step Wait (Timer) is the last action within a kinetic run, the wait time will be ignored in the last cycle. Incubation Incubation is always done at the heating position to ensure inside the instrument proper temperature distribution.
2. Measurement Parameter Editor 2.3 Workflow Pane The main window in i-control is the Workflow pane, where the measurement script is visible and where parameters are defined and edited. There are two ways to insert a program element from the Control bar into the Workflow pane: • Select a program element from the Control bar; by double-clicking it, it is inserted into the Workflow pane directly after the previous program element.
2. Measurement Parameter Editor 2.3.1 Hierarchy of Elements The hierarchy of elements in the Workflow pane is as follows: 1. Plate 2. Part of Plate (Range) 3. Well Any desired measurement step can be inserted directly after a plate, range or well element. Use the Release and Indent options in the Edit menu to modify the sequence of execution of the single strip component. Select an element in the Workflow pane, click the right mouse button and select Release or Indent.
3. Defining Measurements 3. Defining Measurements The following chapter describes some examples to illustrate the definition of different measurements. The Infinite M1000 instrument offers the Quick-Start-Script button in the front right corner on the top cover of the instrument. It may be used to start favorite measurement scripts directly from the instrument. 3.1 Defining End Point Measurements The following example describes an Absorbance End Point Measurement in all wells of a 96 well plate: 1.
3. Defining Measurements If the plate shall be moved out of the instrument after measurement, insert a Move Plate program element and select the Out radio button. If a Move Plate program element is not defined after the measurement, the plate will stay inside the instrument until Move Plate Out is clicked. After finishing the definition as described above start the measurement by clicking button on the toolbar.
3. Defining Measurements 3.1.1 Plate Size – Part of the Plate Use the Plate program element in the workflow pane to choose a plate format. Select the desired plate format from the Plate definition drop-down list (e.g. a black Greiner 96 well plate). To measure a particular well or a range of wells on the plate click the link Use a part of the plate. In the Part of Plate program element click the desired well or drag a frame over the range of desired wells (e.g. A1 to F7).
3. Defining Measurements 3.2 Defining Multilabel Measurements Multilabel measurements are measurements with multiple consecutive reading modes, e.g. with multiple absorbance, fluorescence, luminescence labels or with mixed measurements. The following example describes the definition of a multilabel measurement in a 384 well plate: 58 Instructions for Use for i-control No. 30018668 Rev. No. 1.
3. Defining Measurements Label 1 – Absorbance Measurement in all wells 2011-03 1. Select a 384 well plate (e.g. Greiner 384 Flat Transparent) from the Plate definition drop-down list; select all wells in the Part of Plate. 2. 3. Insert the Absorbance program element from the Control bar, and define as follows: Wavelength/Measurement: 492 nm 4. Read/Number of reads: 25 Instructions for Use for i-control No. 30018668 Rev. No. 1.
3. Defining Measurements Label 2 - Fluorescence Intensity in all wells 1. Insert the Fluorescence Intensity program element from the Control bar and define as follows: 2. Wavelength/Excitation: 483 nm 3. Wavelength/Emission: 535 nm 4. Read/Number of reads: 25 5. Gain: Optimal 60 Instructions for Use for i-control No. 30018668 Rev. No. 1.
3. Defining Measurements Label 3 – Fluorescence Intensity in all wells 1. Insert a second Fluorescence Intensity program element from the Control bar and define as follows: 2. Wavelength/Excitation: 612 nm 3. Wavelength/Emission: 670 nm 4. Read/Number of reads: 25 5. Gain: Optimal After finishing the definition as described above start the measurement by clicking the button on the toolbar. When clicking the Start button, Excel opens automatically and the results are displayed in a worksheet.
3. Defining Measurements 3.3 Defining Kinetic Measurements The following example describes a kinetic measurement of a 96 well plate: 1. Select the 96 well plate (e.g. Greiner 96 Flat Transparent) from the Plate definition drop-down list, and select all wells in the Part of Plate program element. 2. Double-click the Kinetic Cycle program element and define as follows: 3. Cycles/Number of cycles: 50 4.
3. Defining Measurements Use Gain Regulation (available for all Infinite readers) The command Use gain regulation is only available for plate-wise kinetic measurements in fluorescence top/bottom and fluorescence polarization mode. Upon activating Use gain regulation, fluorescence kinetic measurements with increasing signals are prevented from running into “OVER” once the samples produce too high RFU values.
3. Defining Measurements 3.3.1 Defining Well Kinetic Measurements with Injections A Kinetic Measurement means that the whole plate is measured in several consecutive cycles with the same settings. To define a Well Kinetic, select Well from the Control bar by double-clicking or drag the Well program element from the Control bar into the Workflow pane and drop it between Part of Plate and Kinetic Cycles. If necessary, a Kinetic interval can be defined.
3. Defining Measurements 3.3.2 The Difference between “Inject” and “Dispense” The action which is associated with inserting one of these program elements is identical: a defined volume of a liquid is injected into each selected well. The only difference is the workflow: Injecting is done well-wise, which means that the liquid is injected into the first well, and then this well is measured as defined, before the liquid is injected into the second well and so on.
3. Defining Measurements 3.4 Indenting and Releasing Program Elements The decision to indent/ release a program element will modify the workflow of the instrument during measurements. The actions of all program elements with the same indentation are performed sequentially. The only dependence between these program elements is that the next action starts directly after the previous action is finished.
3. Defining Measurements The above definition results in the following workflow: The Absorbance of all wells of a 96 well plate is first measured at 260 nm and then at 280 nm. Both Absorbance measurements are performed in 5 kinetic cycles. Indenting the second Absorbance program elements on a level with Kinetic Cycle item changes the workflow. Select the second Absorbance program element and click the right mouse button. Select Release Strip from the context sensitive menu.
4. Menus 4. Menus 4.1 Menu Bar 4.1.1 File Menu New This command opens a new measurement workflow. If an empty document is to be opened, you will be asked to save the current workflow. Click Yes to save the current workflow or click No to create a new workflow without saving the previous one. Click Cancel to leave the dialog box. Open This command opens an existing i-control workflow (*.mdfx) from the selected folder.
4. Menus Exit This command exits and closes the program. If you are still connected to an instrument, you will be asked if you want to disconnect and to close the program. Click Yes if you want to exit or click No if you want to return to the program. 4.1.2 Edit Menu Cut This command cuts the selected program element, which can be pasted again. Copy This command copies the selected program element. Paste This command pastes the selected program element.
4. Menus 4.1.4 Instrument Menu Disconnect/Connect This toggle command connects or disconnects an instrument to or from i-control. To connect to an instrument select the instrument name from the list. Start This command starts the measurement process. If the measurement is started, a small window informs that the measurement is in progress. Excel opens automatically and the results are displayed in a worksheet.
4. Menus Z-Position For a detailed description of optimizing the Z-position, refer to the Instructions for Use of the Infinite F500, M1000 and M200 Pro. Stacker Control If the reader is connected to a Connect stacker, the Stacker Control option appears in the Instrument menu. • Select Restack to return the processed plates from the output stack to the input stack in their original order.
4. Menus With the Infinite M1000 instrument, only the built-in stacker can be used. If the instrument is connected to a stacker, the Stacker Control option appears in the Instrument menu: • Select Restack to return the processed plates from the output stack to the input stack in their original order. After Restack is selected, a dialog box appears in which the plate type must be selected and confirmed with OK, before the restacking procedure is performed.
4. Menus 4.1.5 Settings Menu Injectors... This command opens the injector maintenance dialog box containing the following procedures: Prime (Example for the Infinite F500) Select injector A, B or both A and B. Depending on which injector is selected the corresponding group box can be edited. Select the Prime Volume and the Prime Speed depending on the instrument connected. Click Start prime to start the priming procedure.
4. Menus Backflush (Example for the Infinite F500) Select injector A, B or both A and B. Depending on which injector is selected the corresponding group box can be edited. Select the Piston Strokes and the Backflush Speed depending on the connected instrument. One piston stroke corresponds to the total volume of the used injector syringe. Click Start backflush to start the reagent backflush procedure. Refer to the Instructions of Use of the instrument connected for further details and examples.
4. Menus Wash (Example for the Infinite F500) Select injector A, B or both A and B. Depending on which injector is selected the corresponding group box can be edited. Select the Piston Strokes and the Wash Speed depending on the connected instrument. Click Start wash to start the washing procedure. Waste Tub Click Empty Waste Tub only when the waste tub has been emptied manually. The software will then alert the user if the waste tub needs to be emptied again.
4. Menus Filter Definitions (Infinite F200, F200 Pro, F500) Select the appropriate filter position and enter the new wavelength, bandwidth, and measurement mode for each new filter: Measurement Mode: Choose from the dropdown list ‘FI’ for fluorescence intensity, ‘ABS’ for absorbance measurements, FP for fluorescence polarization and ‘Empty’ for filter-free positions. Wavelength: Enter the filter wavelength.
4. Menus Plate Definition... This command allows you to choose a plate file from the drop-down list of available plates. The plate definition files contain all relevant parameters of a specific plate type, e.g. coordinates of measurement points, number of columns, number of rows, well form, well diameter, plate height, plate height with cover…). A graphic element at the bottom of the dialog visualizes the parameter which is currently defined.
4. Menus Manufacturer / Pdfx-Name Cat.-No. Drawing-No.
4. Menus Manufacturer / Pdfx-Name 80 Cat.-No. Drawing-No.
4. Menus Manufacturer / Pdfx-Name Cat.-No. Drawing-No.
4. Menus Manufacturer / Pdfx-Name Cat.-No. Drawing-No.: 236 105 236 107 236 108 436 110 436 111 BD Falcon BD24_FluoroBlok 351155 351156 351157 351158 PD801712 BD96_FluoroBlok 351161 351162 351163 351164 MTP-0006 - MTP-0007 Tecan NanoQuantPlate To make a custom plate definition file, choose one from the list as a template. After the appropriate settings have been defined, save it under a different name. Click Save as to save the selected plate definition as a *.pdfx-file. User Settings...
4. Menus Tab Start Up: Behavior at start up can be set. 1. Select a default plate. 2. Determine if the workflow pane should start with an empty workflow, plate only, or plate and part of plate. 3. Select whether the last used instrument should be reconnected 4. Select whether the ‘Open Template’ dialog at startup should be skipped. Tab General: General options can be set. 1. Ask to save the workflow (when changed) before the measurement starts. 2.
4. Menus Result Presentation... This command offers the following tabs to determine the output settings of the measured results in Excel: Depending on the connected instrument, different tabs are visible. The Infinite F500 and M1000 have for example an additional tab for fluorescence polarization. 84 Instructions for Use for i-control No. 30018668 Rev. No. 1.
4. Menus General Presentation: Destination: Select between New workbook, New worksheet, Use previous worksheet or Use existing workbook. If New workbook is selected, a new workbook is opened every time a measurement script is performed. If New worksheet is selected, a new worksheet of the existing workbook is created. If no workbook is open a new one is created. If Use existing workbook is selected, a workbook and a worksheet must be selected.
4. Menus Kinetic Result: Rotation: Select between Columnwise or Rowwise. If Columnwise is selected, the results are displayed in a column (in the Excel sheet). If Rowwise is selected, the results are displayed in a row (in the Excel sheet). Align: Select between A1A2 and A1B1. If A1A2 is selected, the results are arranged in rows (of the microplate). If A1B1 is selected, the results are arranged in columns (of the microplate). Display Times: Select between Time per cycle and Time per well.
4. Menus Exception History… The Exception History dialog box shows a list of exceptions (instrument errors, software failures) with date and time. Every time an exception occurs and an error box is displayed, all relevant information is collected and saved in a zip-file. Each of these zip-files leads to an entry in this list. Relevant information is: The error message and number, communication log-files and system information (like operating system version, free amount of disc space).
4. Menus 4.
4. Menus Select Quickstart Script (M1000 only) The currently visible workflow can be saved and started directly from the instrument: When the favorite script has been saved and is active in the text field, pressing the Quick-Start-Script button on the instrument will start this script. Saved favorite scripts can also be deleted. 2011-03 Instructions for Use for i-control No. 30018668 Rev. No. 1.
5. Batch Processing 5. Batch Processing 5.1 Introduction If the reader is connected to a Connect stacker, it is possible to perform batch processing. The defined i-control script will be performed on each of the available plates in the input stack. CAUTION DO NOT USE MICROPLATES WITH COVERS, WHEN USING THE CONNECT STACKER TO PERFORM BATCH PROCESSING. Note The defined script will be performed on each of the available plates in the input stack.
5. Batch Processing 5.3 Start Stacker Run Once a script has been defined, batch processing can be started by selecting Start Stacker Run from the Instrument menu or by clicking the button on the toolbar. The output stack of the Connect stacker must be empty before starting a run. The Stacker Operations dialog box appears: • Select Skip topmost plate if the plate shall be neglected for measurement. The topmost plate will not be processed and will be moved to the output stack.
5. Batch Processing 5.4 Restacking The Infinite M1000 allows restacking of plates without a preceding measurement. Restacking is also possible when the input stack contains plates. 5.5 Stacker Kinetics (available for Infinite F500 and M1000) In contrast to kinetic measurements on one plate, stacker kinetics allow for the analysis of multiple plates in a time-dependent manner.
Index Index A Alias ................................................................. 73 B Batch Processing ............................................. 91 C Changing Plate Size ........................................ 57 Comment ......................................................... 50 Connected Instrument ..................................... 16 Control Bar Actions ......................................................... 43 Kinetic ..........................................................
Index Start Stacker Run ............................................ 92 T Teaching .......................................................... 72 Temperature .................................................... 43 Toolbar ............................................................. 88 U User Request ................................................... 50 User Settings... ................................................ 82 96 W Wait (Timer) .....................................................
