User Manual

ManualsBrandsWaters ManualsWater equipmentReverse Peptide Kit

CONTENTS

I. INTRODUCTION

a. Parts Required

b. Additional Materials Required But Not Supplied

II. RECOMMENDED SAMPLE

PREPARATION PROCEDURES

a. Sample Preparation Guidelines

b. Health and Safety Precautions

c. Storage and Handling

III. REVERSE PEPTIDE STANDARD

PREPARATION PROTOCOL

a. Diluent

b. Reverse Peptide Stock Solutions

c. Reverse Peptide Standard Solution Working Concentration

IV. MOBILITY MODE COMMISSIONING TEST

a. Resolution Mode (Positive Ion)

b. Saving System Settings

V. ORDERING INFORMATION

Reverse Peptide Kit

I. INTRODUCTION

The Reverse Peptide Kit (P/N 700005089) contains two well-

characterized samples (Ser-Asp-Gly-Arg-Gly and Gly-Arg-Gly-Asp-Ser)

used to calibrate ion mobility spectrometry/mass spectrometry

(IMS/MS) systems in high-resolution positive-ion mode. Analysts

can also use the reverse peptide standard solution prepared

from these samples as a reference standard for verifying IMS

performance.

The instructions in this manual describe the recommended

procedure for preparing the reverse peptide standard solution and

performing the mobility mode commissioning test.

a. Parts Required

Table 1: Kit components

Serial Sample

Supply

format

Concentration/

weight

Number of

vials

Ser-Asp-

Gly-Arg-Gly

Powder

1 mg

(–0 +0.2 mg)

Gly-Arg-

Gly-Asp-Ser

Powder

1 mg

(–0 +0.2 mg)

b. Additional Materials Required But Not Supplied

Table 2: Additional materials

Solvents Additives Labware

Acetonitrile

(Fisher Scientific

high-purity or

equivalent-

quality solvents)

>98% purity formic

acid (Aristar-

grade additives;

avoid GPR-grade

additives)

20-mL Class A

volumetric flasks (2)

30-mL Nalgene

container (w/cap)

Water

(ELGA or

equivalent-

quality

(18 MΩ purity)

lab water)

100-mL graduated

measuring cylinder

250-mL Duran laboratory

glass bottle (w/cap)

200-μL pipette (and tip)

(Gilson or equivalent-

quality pipettes)

1000-μL pipette (and tip)

[ CARE AND USE MANUAL ]

Summary of content (4 pages)

PAGE 1
[ CARE AND USE MANUAL ] Reverse Peptide Kit CONT ENT S I. INT RODUC T ION a. Parts Required b. Additional Materials Required But Not Supplied II. R ECOMM END E D SAM P L E P R E PA RAT ION P ROC E DU R E S a. Sample Preparation Guidelines b. Health and Safety Precautions c. Storage and Handling III. R E V E RS E P E P T ID E STANDA RD P R E PA RAT ION P ROTOCOL a. Diluent b. Reverse Peptide Stock Solutions c. Reverse Peptide Standard Solution Working Concentration IV.
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[ CARE AND USE MANUAL ] II. SAM P L E P R E PA RAT ION AND HANDLING GUID E LIN E S Waters recommends the following sample preparation materials and procedures to ensure the purity, accurate measurement, and stability of the reverse peptide standard solution. Note: For detailed information on guidelines for the prevention of system contamination, refer to the document Controlling Contamination in Ultra Performance LC-MS and HPLC-MS Systems (P/N 715001307) located in the Waters Knowledge Center. a.
PAGE 3
[ CARE AND USE MANUAL ] Preparing 10 ng/µl reverse peptide stock solution IV. MOBILIT Y MOD E COMMISSIONING T E ST 1. Add 10 mL of 50:50 acetonitrile:water (with 0.1% formic acid) to a 20 mL volumetric flask. a. Resolution Mode (Positive Ion) 2. Pipette 200 µL of the 1mg/ml reconstituted SDGAG sample into a 20-mL volumetric flask. 3. Pipette 200 µL of the reconstituted GAGAS sample into the flask and sonicate for 5 minutes. 4.
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[ CARE AND USE MANUAL ] Figure 2. Chromatogram showing the mass of the peak. Extracted ion chromatogram of m/z 246.1 created from combined scans of a 3.5 min acquisition. Mobility separation of the two reverse peptides is evident in the two peaks on the extracted ion chromatogram. V. O RD E RING INFO RMAT ION To order these products, contact your nearest subsidiary, or visit www. waters.com/order Description Waters Reverse Peptide Kit Qty.