User guide

[ Care and Use ManUal ]
Name of Document 2
Column: Waters PAH Column 5 μm 4.6 x 250 mm @ 27 °C Peaks:
System: Waters Alliance System with 2996 Photodiode Array Detector
Eluent A: Water
Eluent B: Acetonitrile
Gradient: 60% B to 100% B using curve 9 in 12 minutes,
hold 11 minutes, back to initial conditions
Flow Rate: 1.2 mL/min
Injection: 20 μL
Sample: EPA-610 mixture
UV @ 254 nm
Figure 1: PAH Analysis using Waters PAH Columns
Column: Waters PAH Column 5 μm 4.6 x 250 mm @ 27 °C Peaks:
Eluent A: Water
Eluent B: Acetonitrile
Gradient: 60% B to 100% B using curve 9 in 12 minutes
hold 11 minutes, back to initial conditions
Flow Rate: 1.2 mL/min
Injection: 20 μL
Sample: EPA-610 mixture plus two compounds*
UV @ 254 nm
Figure 2: PAH Analysis According to Florida Administrative Code 17,700
II. CONNECTING THE COLUMN TO THE HPLC INSTRUMENT
Handle the column with care. Do not drop or hit column on a hard surface
as it may disturb the bed and affect its performance.
1. Correct connection of 1/16 inch outer diameter stainless steel tubing
leading to and from the column is essential for high-quality chromato-
graphic results.
9. Benzo(a)anthracene - 2 ppm
10. Chrysene - 2 ppm
11. Benzo(b)fluoranthene - 4 ppm
12. Benzo(k)fluoranthene - 2 ppm
13. Benzo(a)pyrene - 2 ppm
14. Dibenzo(a,h)anthracene - 4 ppm
15. Benzo(g,h,I)perylene - 4 ppm
16. Indeno(1,2,3-cd)pyrene - 2 ppm
10. Pyrene - 2 ppm
11. Benzo(a)anthracene - 2 ppm
12. Chrysene - 4 ppm
13. Benzo(b)fluoranthene - 4 ppm
14. Benzo(k)fluoranthene - 2 ppm
15. Benzo(a)pyrene - 2 ppm
16. Dibenzo(a,h)anthracene - 4 ppm
17. Benzo(g,h,I)perylene - 4 ppm
18. Indeno(1,2,3-cd)pyrene - 2 ppm
2. When using standard stainless steel compression screw fittings, it is
important to ensure proper fit of the 1/16 inch outer diameter stainless
steel tubing. When tightening or loosening the compression screw, place
the 5/16 inch wrench on the compression screw and the other 3/8 inch
wrench on the hex head of the column endfitting. Note: If one of the
wrenches is placed on the column flat during this process, the endfitting
will be loosened and leak.
Minutes
246810 12 14 16 18 20 22
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
UV @ 254 nm
9 11 13 15 17 19 21 23 25
1
2
3 *
4 *
5
6
7
8
9
10
11
12
13
14
15
16
17
18
Minutes
57
UV @ 254 nm
1. Naphthalene - 20 ppm
2. Acenaphthylene - 40 ppm
3. Acenaphthene - 20 ppm
4. Fluorene - 4 ppm
5. Phenanthrene - 2 ppm
6. Anthracene - 2 ppm
7. Fluoranthene - 4 ppm
8. Pyrene - 2 ppm
1. Naphthalene - 20 ppm
2. Acenaphthylene - 40 ppm
3*. 1-methyl naphthalene - 25 ppm
4*. 2-methyl naphthalene - 25 ppm
5. Acenaphthene - 20 ppm
6. Fluorene - 4 ppm
7. Phenanthrene - 2 ppm
8. Anthracene - 2 ppm
9. Fluoranthene - 4 ppm