Manual

ManualsBrandsWaters ManualsWater equipmentiKey

[ CARE AND USE MANUAL ]

CONTENTS

I. INTRODUCTION

II. iKEY FEATURES

a. Analytical iKey

b. Accessory iKeys

c. iKey Component Description

III. CONNECTING THE iKEY TO THE

ionKEY SOURCE

IV. iKEY USAGE

a. Sample Preparation

b. Mobile Phases

c. pH Range

d. Pressure

e. Temperature

V. REMOVING THE iKEY FROM THE

ionKEY/MS SYSTEM

VI. CARING FOR THE iKEY

a. Cleaning and Regeneration

b. Storage

c. Good Practices

VII. TROUBLESHOOTING iKEY PERFORMANCE

VIII. ORDERING INFORMATION

iKey Separation Device

I. INTRODUCTION

The Waters iKey™ Separation Device is a high-pressure,

microfluidic device that provides UPLC

separations with

the ionKey/MS™ System. The iKey eliminates manual fitting

connections and minimizes the potential for manual variability

and extra-column dispersion.

The iKey contains a ceramic substrate produced using a modified,

co-fired ceramic manufacturing process which contains an

analytical separation channel packed with stationary phase, an

electrospray emitter, a column heater, and an integrated memory

device. The integrated memory maintains the number of injections,

maximum backpressure, and number of sample sets run. A circuit

in the iKey senses and controls temperature as well.

The iKey is manufactured to exacting specifications, providing

outstanding peak symmetry for maximum sensitivity and

accurate quantitation. Each iKey is individually tested to

ensure that it passes stringent quality control requirements.

Compared to conventional chromatographic systems, successful

day-to-day performance of the ionKey/MS System requires

certain considerations. This document provides several essential

recommendations for the successful use of the iKey. The iKey

is the only analytical consumable device compatible with

ionKey/MS System.

Summary of content (11 pages)

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[ CARE AND USE MANUAL ] iKey Separation Device CONT ENT S I. INT RODUC T ION II. iK E Y F EAT U R E S a. Analytic al iKey b. Accessory iKeys c. iKey Component Description III. CONN EC T ING T H E iK E Y TO T H E ionK E Y SOU RC E IV. iK E Y USAG E a. Sample P reparation b. Mobile P hases c. pH Range d. P ressure e. Temperature V. R EMOV ING T H E iK E Y F ROM T H E ionK E Y/MS SYST EM V I. C A RING FO R T H E iK E Y a. C leaning and Regeneration b. Storage c. Good P ractices V II.
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[ CARE AND USE MANUAL ] II. iK E Y F EAT U R E S a. Analytical iKey This iKey has a 150 µm x 50 mm or 150 μm x 100 mm analytical separation channel available with a variety of packing materials. An electrospray emitter is located at the end of the channel. A sheath is provided to protect the iKey and its emitter from damage during handling.
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[ CARE AND USE MANUAL ] b. Accessory iKeys Flow Injection iKey: This iKey has no packing material. It enables one to inject a sample from the sample manager directly to the emitter on the iKey without any chromatographic separation. Flow path is identical to the 50 mm Analytical iKey. Infusion iKey: This iKey enables calibrant infusion from the ionKey/MS fluidics.
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[ CARE AND USE MANUAL ] III. CONN EC T ING T H E iK E Y TO T H E ionK E Y SOU RC E These procedures outline the steps necessary to connect your iKey into the ionKey source. Connecting your iKey properly is essential for operation. To insert the iKey in the ionKey source: 1. Ensure the lever is vertical, and the unlock indicator is in the 12 o’clock position. Unlock indicator in 12 o’clock position Lever in vertical position 2. Remove the iKey from the protective sheath. 3.
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[ CARE AND USE MANUAL ] IV. iK E Y USAG E To ensure the continued high performance of your iKey Separation Device, please follow these guidelines: a. Sample Preparation 1) Samples containing hundreds of nanograms of undigested protein (and higher) per injection have been shown to limit or shorten iKey lifetimes. Therefore, it is essential that proper sample preparation protocols are followed.
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[ CARE AND USE MANUAL ] 5) Consider filtering samples with a 0.2 µm membrane to remove particulates. If the sample is dissolved in a solvent that contains an organic modifier (e.g., acetonitrile, methanol, etc.) ensure that the membrane/filter material is compatible with the solvents in use. Alternatively, centrifuge the sample for 20 minutes at 8000 RPM, followed by the transfer of the supernatant to an appropriate Waters TruView LCMS Certified Vial could be considered.
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[ CARE AND USE MANUAL ] b. Mobile Phases To maintain and ensure maximum iKey performance, use high quality chromatography solvents. Use only filtered and degassed mobile phases. Filter all aqueous buffers prior to use through a 0.2 μm filter. Solvents containing suspended particulate materials will generally clog the iKey ceramic substrate (analogous to clogging the inlet frit of an LC column). This may result in higher backpressure or distorted peak shape.
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[ CARE AND USE MANUAL ] V. R EMOV ING T H E iK E Y F ROM T H E ionK E Y SOU RC E CAUTION: Opening the locking lever on the ionKey system without first stopping flow and decompressing the fluidic pressure can damage the iKey. 1. Stop the solvent flow and decompress the fluidic pressure on the iKey using one of these methods. a. Press the STOP FLOW button on the front of the source. b. In the Console, select Sample Manager from the system tree, and then click > Maintain > Remove iKey. 2.
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[ CARE AND USE MANUAL ] b. Storage For periods longer than four days, store the reversed-phase iKey in 100% acetonitrile. Do not store iKeys in buffered eluents. If the mobile phase contained a buffer salt, flush the reversed-phase iKey with 10 iKey volumes of HPLC grade water followed by 10 iKey volumes of acetonitrile. Failure to perform this intermediate step could result in precipitation of the buffer salt in the column when 100% acetonitrile is introduced.
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[ CARE AND USE MANUAL ] ■■ Don’t assume the iKey is to blame: ■■ ■■ ■■ ■■ ■■ ■■ Investigate cause of iKey failure; Monitor backpressure; Examine mobile phase age, bacterial contamination, mobile phase precipitation, etc.; Examine sample quality; Examine injection solvent strength. Do not prepare excessive amounts of mobile phase: ■■ To reduce the chances of mobile phase contamination or degradation, prepare enough mobile phase to last for 3–4 days.
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[ CARE AND USE MANUAL ] V III. O RD E RING INFO RMAT ION Product Description Part Number iKey, Peptide BEH C18, 130Å, 1.7 µm, 150 µm x 50 mm* 186006764 iKey, BEH C18, 130Å, 1.7 µm, 150 µm x 50 mm 186007256 iKey, Peptide CSH C18, 130Å, 1.7 µm, 150 µm x 50 mm* 186007257 iKey, CSH C18, 130Å, 1.7 µm, 150 µm x 50 mm iKey, BEH C18, 300Å, 1.7 µm, 150 µm x 50 mm 186007244 iKey, Peptide BEH C18, 130Å, 1.7 µm, 150 µm x 100 mm* iKey, BEH C18, 130Å, 1.