Reference Material Manual

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[ CARE AND USE MANUAL ]
HILIC Quality Control Reference Material
II. STORAGE AND STABILITY
The standard mix comes in a Waters crimp top vial for direct injection and contains 1 mL total in 90:10 acetonitrile:100 mM ammonium
formate pH 3.0 buffer.
The standard mix is shipped at ambient temperatures. It is highly recommended that, upon receipt, the standard should be refrigerated at
4 °C for short term storage and for long term storage frozen.
III. EXAMPLE OF USING THE HILIC QCRM
Figure 2. Chromatographic Resolution using the HILIC QCRM.
Figure 3. HILIC QCRM across different particle sizes.
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HILIC QCRM
Lot# W11061301
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1. Acenaphthene 2. Thymine 3. Adenine 4. Cytosine
1. Acenaphthene 2. Thymine 3. Adenine 4. Cytosine
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ACQUITY UPLC BEH Amide, 1.7 µm
2.1 x 150 mm
XBridge BEH Amide XP, 2.5 µm
2.1 x 150 mm
XBridge BEH Amide, 3.5 µm
2.1 x 150 mm
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Below is a reference chromatogram with conditions to help provide
an example of using the HILIC QCRM as a benchmark for system
performance.
System Information
Mobile phase (A1) 90% acetonitrile/10% 100mM
ammonium formate pH 3.0 (v/v), (A2) not
used, (B1) not used, (B2) not used
Samples:
Acenaphthene 19.0 µg/mL
Thymine 3.7 µg/mL
Adenine 3.7 µg/mL
Cytosine 7.7 µg/mL
Detection wavelength 254 nm
Temperature 30 °C
System ACQUITY UPLC
Weak needle wash 90/10 (v/v) MeCN/Milli-Q
®
Water
Strong needle wash 10/90 (v/v) MeCN/Milli-Q Water
Injection volume (µL) 2.0
Flow rate (mL/min) 0.5
Seal wash 20% MeCN/80% (v/v) Milli-Q water
Data acquisition Empower
®
2 Software