Owner's manual

[ Care and Use ManUal ]
BioSuite Peptide Analysis Columns 5
Inapropertubing/columnconnection(Figure2),thetubingtouchesthe
bottom of the column endfitting, with no void between them.
Tubing touches the bottom of the
column endfitting, with no void between them.
Figure 2: Proper Tubing / Column Connection
The presence of a void in the flow stream reduces column performance.
This can occur if a Parker ferrule is connected to a Waters style endfitting
(Figure3).
A void appears if the tubing with a Parker
ferrule is connected to a Waters style column.
Figure 3: Parker Ferrule in a Waters Style Endfitting
There is only one way to fix this problem: Cut the end of the tubing with the
Parker ferrule, place a new Waters ferrule on the tubing and make a new con-
nection. Before tightening the screw, make sure that the tubing bottoms out
in the endfitting of the column. Conversely, if tubing with a Waters ferrule is
connected to a column with Parker style endfitting, the end of the tubing will
bottom out before the ferrule reaches its proper sealing position. This will
leaveagapandcreatealeak(Figure4).
The connection leaks if a Waters ferrule is
connected to a column with a Parker style endfitting.
Figure 4: Waters Ferrule in a Parker Style Endfitting
There are two ways to fix the problem:
1. Tighten the screw a bit more. The ferrule moves forward, and reaches
the sealing surface. Do not over tighten since this may end in breaking
the screw.
2. Cutthetubing,replacetheferruleandmakeanewconnection.
Alternatively, replace the conventional compression screw fitting with
anall-in-onePEEKfitting(WaterspartnumberPSL613315)thatallows
resetting of the ferrule depth. Another approach is to use a Keystone,
Inc.SLIPFREE
®
connector to always ensure the correct fit. The finger tight
SLIPFREEconnectorsautomaticallyadjusttofitallcompressionscrewtype
fittingswithouttheuseoftools,(Figure5).
Figure 5: Single and Double SLIPFREE Connectors
Features:
 • Tubingpushedintoendfitting,therebyguaranteeingavoid-free 
connection
 • Connector(s)come(s)installedontubing
 • Varioustubingi.d.andlengthsavailable
 • Fingertightto10,000psi–Neverneedswrenches
 • Readjuststoallcolumnendfittings
 • Compatiblewithallcommerciallyavailableendfittings
 • Uniquedesignseparatestube-holdingfunctionfromsealing 
function
Table 2. Waters Part Numbers for SLIPFREE Connectors
SLIPFREEType
and Tubing
Length 0.005”
TubingInternal
Diameter
0.010” 0.020”
Single 6 cm PSL 618000 PSL 618006 PSL618012
Single 10 cm PSL618002 PSL 618008 PSL618014
Single20cm PSL618004 PSL 618010 PSL 618016
Double 6 cm PSL 618001 PSL 618007 PSL 618013
Double 10 cm PSL 618003 PSL 618009 PSL 618015
Double20cm PSL 618005 PSL 618001 PSL 618017
Void
Gap