Owner manual

[ Care and Use ManUal ]
Waters Advanced Purification Columns 2
a. AP Column Accessories
The following accessories are shipped with the AP column:
• Packingfunnel
• Endplugsforcolumnstorage
• SparePartsKitconsistingof:
• 1inletO-ring
• 1outletO-ring
• 1connectortipO-ring
• 1packingfunnelO-ring
• 2filterassemblies
• 2ferrules
• 10gripperringtools
• 1Allenwrench
Table 1: AP Column Specifications
II. usIng the Column
a. Safety and Solvent Considerations
Inherent surface imperfections can weaken glass at high pressures.
Maintain lower pressures to prolong column life. The AP glass
column has an external plastic jacket which acts as a safety shield.
Toavoidcolumnhardwaredamage,neverexceed10MPa(1500psi
or100atm)insidethecolumn.
High backpressures may damage resin-based packing materials.
Consult your packing material care and use manual for packing
material backpressure limits.
AP column materials are compatible with most solvents used in
HPLC. However:
• Certainsolvents,suchasacetoneorchlorinatedhydrocarbons, 
may reduce the life expectancy of the O-rings.
• Thetransparentplasticjacketmayweakenifexposedtocertainorganic
solvents. If the jacket crazes, replace it.
b. Adjusting the Plunger
Range of movement
The packed bed is at maximum volume when the hex at the inlet end
of the plunger is flush with the top of the coarse/fine adjustment
slide. Do not withdraw the plunger further, or too few threads will be
engaged to ensure reliable operation.
The slide must be engaged against the locking hex while the column
is in use to prevent the plunger from unscrewing under pressure.
Figure 2: Column at Maximum Volume
Internal diameter 10mm
Length (nominal) 100,200,300,or600mm
Maximum operating pressure 10MPa(100Atm,1500psi)
Filter size 20µm
Materials along fluid path:
Column
Tubing
Filter
Connectors
O-rings
Borosilicate glass
Tefzel
®
Polyethylene
Ryton-4
®
(polyphenylene sulfide resin)
Buna-N
Operating temperature 4°Cto50°C
CorrectIncorrect Incorrec
tI
ncorrect
Inlet Hex is Flush
with Top of Slide
Threads
Exposed
Locking Hex
Exposed
Inlet Hex
Exposed