Owner's manual

ManualsBrandsWaters ManualsWater equipmentACQUITY UPLC HSS Columns

[ Care and Use ManUal ]

XSELECT HSS HPLC COLUMNS

Contents

I. GettInG started

a. Column Connectors

b. Column Installation

c. Column Equilibration

d. eCord Installation

e. Initial Column Efficiency Determination

f. VanGuard

™

Pre-Columns

II. Column use

a. Sample Preparation

b. pH Range

c. Solvents

d. Pressure

e. Temperature

III. Column CleanInG, reGeneratInG and storaGe

a. Cleaning and Regeneration

b. Storage

IV. IntroduCInG ECord IntellIGent ChIp

teChnoloGy

a. Introduction

b. Installation

c. Manufacturing Information

d. Column Use Information

V. addItIonal InformatIon

a. Tips for Maximizing ACQUITY UPLC HSS

Column Lifetimes

b. Recommended Flow Rates and Backpressures

for Reversed-Phase ACQUITY UPLC HSS Columns

Thank you for choosing a Waters ACQUITY UPLC

HSS column. The

ACQUITY UPLC HSS packing materials were designed specifically

for use with the Waters ACQUITY UPLC system and are manufactured

in a cGMP, ISO 9001:2000 certified plant using ultra pure reagents.

Each batch of ACQUITY UPLC HSS material is tested chromato-

graphically with acidic, basic and neutral analytes and the results

are held to narrow specification ranges to assure excellent, repro-

ducible performance. Every column is individually tested and a

Performance Chromatogram and Certificate of Batch Analysis are

provided on the eCord

™

intelligent chip.

ACQUITY UPLC columns were designed and tested specifically for

use on the ACQUITY UPLC system. ACQUITY UPLC columns will

exhibit maximum chromatographic performance and benefits ONLY

when used on the holistically-designed ACQUITY UPLC system since

the ACQUITY UPLC system and column were created and designed

to operate together. For these reasons, Waters cannot support

the use of ACQUITY UPLC columns on any system other than an

ACQUITY UPLC system.

aCQuIty uplC hss Columns

Summary of content (9 pages)

PAGE 1
[ Care and Use Manual ] ACQUITY UPLC HSS Columns Contents Thank you for choosing a Waters ACQUITY UPLC® HSS column. The ACQUITY UPLC HSS packing materials were designed specifically I. Getting Started for use with the Waters ACQUITY UPLC system and are manufactured a. Column Connectors in a cGMP, ISO 9001:2000 certified plant using ultra pure reagents. b. Column Installation Each batch of ACQUITY UPLC HSS material is tested chromato- c. Column Equilibration d. eCord Installation e.
PAGE 2
[ Care and Use Manual ] I. GETTING STARTED and gives more rapid baseline equilibration. Each ACQUITY UPLC HSS column comes with a Certificate of Analysis and a Performance Test Chromatogram embedded within the eCord intelligent chip. The Certificate of Analysis 4. Gradually increase the flow rate as described in step 2. 5. Once a steady backpressure and baseline have been achieved, proceed to the next section.
PAGE 3
[ Care and Use Manual ] e. Initial Column Efficiency Determination 1. VanGuard™ Pre-Column ACQUITY UPLC® Column Perform an efficiency test on the column before using it. This test may consist of: a) An analyte test mixture that is commonly used in your laboratory, and/or b) An analyte mixture as found on the “Performance Test Place wrench here Chromatogram” which accompanied your column. Note: If b) is performed, the isocratic efficiencies measured in “Performance Test Chromatogram”.
PAGE 4
[ Care and Use Manual ] Table 2: Buffer Recommendations for Using ACQUITY UPLC HSS Columns Volatility (±1 pH unit) Used for Mass Spec 0.3 Volatile Yes Acetic Acid 4.76 Volatile Yes Formic Acid 3.75 Volatile Yes Acetate (NH4CH2COOH) 4.76 3.76 – 5.76 Volatile Yes Formate (NH4COOH) 3.75 2.75 – 4.75 Volatile Yes Phosphate 1 2.15 1.15 – 3.15 Non-volatile No Phosphate 2 7.2 6.20 – 8.
PAGE 5
[ Care and Use Manual ] Note: Working at the extremes of temperature, pressure and/or pH b. For Normal-Phase Conditions will result in shorter column lifetimes. The HSS Cyano column can be used for both reversed-phase separations as well as normal-phase separations. The column is originally shipped in acetonitrile and is ready to use for reversed-phase III. Column Cleaning, Regenerating and Storage conditions. a.
PAGE 6
[ Care and Use Manual ] d. Storage post Normal-Phase use For rapid equilibration upon start-up, it is recommended that you store the HSS Cyano column in the mobile phase that is commonly used for your normal-phase separation. Completely seal the column to avoid evaporation and drying out of the bed. IV. INTRODUCING eCORD INTELLIGENT CHIP TECHNOLOGY a.
PAGE 7
[ Care and Use Manual ] d. Column Use Information The eCord chip provides the customer with column use data. The top of the screen identifies the column including chemistry type, column dimensions and serial number. The overall column usage information includes the total number of samples, total number of injections, total sample sets, date of first injection, date of last injection, maximum pressure and temperature.
PAGE 8
[ Care and Use Manual ] 7. Mobile phase-related questions to ask: • Am I working with pH 7 phosphate buffer (which is • Am I using 100% aqueous mobile phases? Am I able VERY susceptible to bacterial growth)? to add a small amount of organic modifier to my mobile 8. phase A? Sample-related questions to ask: • If I inject neat standards prepared in mobile phase do I • Do I filter my aqueous mobile phases through 0.
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[ Care and Use Manual ] Sales Offices Austria and European Export (Central South Eastern Europe, CIS and Middle East) 43 1 877 18 07 Australia 61 2 9933 1777 Belgium 32 2 726 1000 Brazil 55 11 4134 3788 Canada 1 800 252 4752 China 86 10 5293 6688 Czech Republic 420 2 617 11384 Denmark 45 46 59 8080 Finland 358 9 5659 6288 France 33 1 30 48 72 00 Germany 49 6196 400 600 Hong Kong 852 2964 1800 Hungary 36 1 350 5086 Norway 47 6 384 6050 Poland 48 22 833 4400 Puerto Rico 1 787 747 8445 Russia/CIS 7 495 727 4