User guide

ManualsBrandsWaters ManualsWater equipmentACQUITY UPC2 Trefoil Columns

[ CARE AND USE MANUAL ]

CONTENTS

I. INTRODUCTION

II. GETTING STARTED

a. Safety Considerations

b. Column Connectors and Installation

c. eCord Installation

d. Column Equilibration

e. Initial Column Efficiency Determination

III. COLUMN USE

a. Sample Preparation

b. Solvents

c. Additives

d. Pressure

e. Band Spreading Minimization

IV. TROUBLESHOOTING

V. COLUMN CLEANING, ADDITIVES,

AND STORAGE

a. Cleaning

b. Effects of Additives

c. Storage

VI. INTRODUCING ECORD INTELLIGENT

CHIP TECHNOLOGY

a. Introduction

b. Installation

c. Manufacturing Information

d. Column Use Information

VII. ACQUITY UPC

COLUMN FAMILY

ACQUITY UPC

Trefoil Columns

I. INTRODUCTION

Thank you for choosing an ACQUITY UPC

Trefoil™ Column.

ACQUITY UPC

Trefoil Columns are specifically designed to use

the complete range of capabilities of the ACQUITY UPC

System

to achieve fast, robust chiral separations. ACQUITY UPC

Trefoil

Columns are uniquely designed for the ACQUITY UPC

System

to enable both selectivity and speed in chiral separations and to

reduce method development time. ACQUITY UPC

Trefoil Columns

consist of modified polysaccharide coated stationary phases for

broad-spectrum chiral selectivity. The higher order structure of

the polysaccharides have specific spatial orientations that impart

chiral selectivity through well-chosen modifications or selectors.

ACQUITY UPC

Trefoil AMY1, CEL1, and CEL2 column

chemistries are complementary to each other and independently

offer different retention characteristics for separating chiral

compounds. Selectivity can be further enhanced by using

co-solvents and additive blends that most favorably modulate

chiral recognition. These columns are designed to separate

enantiomers, stereoisomers, metabolites, degradants, and

impurities with greater resolution and speed.

Summary of content (7 pages)

PAGE 1
[ CARE AND USE MANUAL ] ACQUITY UPC 2 Trefoil Columns CONT ENT S I. INT RODUC T ION II. G E T T ING STA RT E D a. Safety Considerations b. Column Connectors and Installation c. eCord Installation d. Column Equilibration e. Initial Column Efficiency Determination III. COLUMN US E a. Sample Preparation b. Solvents c. Additives d. Pressure e. Band Spreading Minimization IV. T ROUBL E SHOOT ING V. COLUMN C L EANING, A DDIT IV E S, AND STO RAG E a. Cleaning b. Effects of Additives c. Storage V I.
PAGE 2
[ CARE AND USE MANUAL ] ACQUITY UPC 2 Trefoil Columns for Chiral Separations O ACQUITY UPC 2 Trefoil AMY1 2.5 µm Columns O Amylose tris-(3,5-dimethylphenylcarbamate) 0.0 0.0 0.0 0.2 0.2 0.2 0.4 0.4 0.4 0.6 0.6 0.6 0.8 0.8 0.8 1.0 1.0 1.0 1.2 1.2 1.2 1.4 1.4 1.4 1.6 min 1.8 2.0 1.6 1.6 min 1.8 2.0min 1.8 2.0 ACQUITY UPC 2 Trefoil CEL1 2.5 µm Columns Cellulose tris-(3,5-dimethylphenylcarbamate) ACQUITY UPC 2 Trefoil CEL2 2.
PAGE 3
[ CARE AND USE MANUAL ] Any small leak could produce a situation where the LEL (lower exposure limit) is reached. Laboratories should be equipped with CO2 and/or O2 sensors when carbon dioxide (CO2) is in use. b. Column Connectors and Installation ACQUITY UPC 2 Systems utilize tubing and connectors which have been designed to meet stringent tolerance levels and to minimize extra-column volume.
PAGE 4
[ CARE AND USE MANUAL ] III. COLUMN US E To ensure the continued high performance of ACQUITY UPC 2 Columns, follow these guidelines: a. Sample Preparation 1. Sample impurities often contribute to column contamination. Waters offers both solid-phase extraction (SPE) and supercritical fluid extraction (SFE) options. For SPE, use Oasis ® Solid-Phase Extraction Cartridges/Columns or Sep-Pak ® Cartridges of the appropriate chemistry to cleanup the sample before analysis. For more information, visit to www.
PAGE 5
[ CARE AND USE MANUAL ] appropriate additive. Additives tend to improve peak shape and control the retention characteristics of analytes, but can also impart different selectivities. It is recommended to flush and remove additives and salts from the column before placing the column into storage. d. Pressure ACQUITY UPC 2 Column hardware and packing materials are packed and tested to pressures of up to 6,000 psi (414 bar or 41 Mpa). e.
PAGE 6
[ CARE AND USE MANUAL ] Retest the column after using the cleaning procedure to determine if the specific problem has been fixed. If so, continue using the column, avoiding samples and solvents that may clog the column inlet. b. Effects of Additives Use of co-solvent additives (e.g. ammonia, organic amines, formic acid, trifluoroacetic acid) can be used successfully with all Trefoil columns. Consider the volatility and detector compatibility when choosing an appropriate additive.
PAGE 7
[ CARE AND USE MANUAL ] c. Manufacturing Information d. Column Use Information The eCord Chip provides the customer with column use data, column dimensions and serial number. The overall column usage information includes the total number of samples, total number of injections, total sample sets, date of first injection, date of last injection, maximum pressure and temperature.