Owner manual

ManualsBrandsWaters ManualsWater equipmentACQUITY UPC2 Torus Columns

[ CARE AND USE MANUAL ]

CONTENTS

I. INTRODUCTION

II. GETTING STARTED

a. Safety Considerations

b. Column Connectors and Installation

c. eCord Installation

d. Column Equilibration

e. Initial Column Efficiency Determination

f. VanGuard Pre-Columns

III. COLUMN USE

a. Sample Preparation

b. Solvents

c. Additives

d. Pressure

e. Useable Flow-Rate Ranges and Expected ACQUITY UPC

System Pressures

f. Band Spreading Minimization

IV. TROUBLESHOOTING

V. COLUMN CLEANING, ADDITIVES,

AND STORAGE

a. Cleaning

b. Effects of Additives

c. Aging

d. Storage

VI. INTRODUCING ECORD INTELLIGENT

CHIP TECHNOLOGY

a. Introduction

b. Installation

c. Manufacturing Information

d. Column Use Information

VII. ACQUITY UPC

COLUMN FAMILY

ACQUITY UPC

Torus Columns

I. INTRODUCTION

Thank you for choosing an ACQUITY UPC

Torus™ Column.

Torus Columns are specifically designed to use the complete

range of capabilities of the ACQUITY UPC

System to achieve fast,

robust achiral separations. ACQUITY UPC

Torus Columns simplify

the method development process with four completely new and

innovative 1.7 µm chemistries for UltraPerformance Convergence

Chromatography.™ These columns are designed for excellent

peak shape that eliminate or reduce need for additives, coupled

with added selectivity for a wide range of compounds and

improved robustness.

These columns are based on new bonding technology used to

create an adaptive selectivity platform. Starting with the ethylene

bridged hybrid (BEH) particle, the patent pending bonding consists

of a two stage functionalization of chromatographic particles.

The first stage controls retention characteristics and is applied

to each sorbent. The individual second stage selectors impart

the observed selectivity and control peak shape.

Summary of content (8 pages)

PAGE 1
[ CARE AND USE MANUAL ] ACQUITY UPC 2 Torus Columns CONT ENT S I. INT RODUC T ION II. G E T T ING STA RT E D a. Safety Considerations b. Column Connectors and Installation c. eCord Installation d. Column Equilibration e. Initial Column Efficiency Determination f. VanGuard Pre-Columns I. INT RODUC T ION Thank you for choosing an ACQUITY UPC 2® Torus™ Column.
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[ CARE AND USE MANUAL ] Achiral Chemistry Stationary Phase Particle Shape Particle Size (µm) Pore Size (Å) Pore Volume (cc/g) Surface Area (m2 /g) Endcapped Torus 2-PIC 2-Picolylamine Spherical 1.7 130 0.7 185 Proprietary Torus DEA Diethylamine Spherical 1.7 130 0.7 185 Proprietary Torus Diol High-density Diol Spherical 1.7 130 0.7 185 Proprietary Torus 1-AA 1-Aminoanthracene Spherical 1.7 130 0.7 185 Proprietary Table 1. Physical Characteristics.
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[ CARE AND USE MANUAL ] c. eCord Installation The eCord button should be attached to the side of the column heater module. The eCord button is magnetized and does not require specific orientation. Empty Column Volumes (mL) Column Internal Diameter (mm) Column Length 2.1 mm 3.0 mm 50 mm 0.2 0.4 75 mm 0.3 0.6 d. Column Equilibration 100 mm 0.4 0.8 ACQUITY UPC 2 Columns are shipped dry. Equilibrate the column with a minimum of 10-column volumes of the mobile phase prior to use.
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[ CARE AND USE MANUAL ] 4. From below, insert VanGuard Pre-column into ACQUITY UPC 2 Column inlet and hand-tighten (collet and ferrule are not yet permanently attached). 5. While pushing the VanGuard Pre-column into the column inlet, turn assembled column and pre-column 180˚ so that precolumn is now on top. 6. Tighten with two 5/16” wrenches placed onto ACQUITY UPC 2 Column flats and VanGuard Pre-column hex nut (male end) as shown above. 7. Tighten 1/4 turn to set collet and ferrule. 8.
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[ CARE AND USE MANUAL ] 100% CO2 60/40 CO2 /MeOH Dimension Predicted Max Flow Rate (mL/min) Predicted Max Pressure (psi) Predicted Max Flow Rate (mL/min) Predicted Max Pressure (psi) 2.1 x 50 mm 3.45 5581 2.00 6011 2.1 x 75 mm 2.65 5970 1.35 5938 2.1 x 100 mm 2.05 5969 1.05 6060 2.1 x 150 mm 1.40 5951 0.70 6002 3.0 x 50 mm 4.00 4179 3.60 5946 3.0 x 75 mm 3.75 4897 2.60 5959 3.0 x 100 mm 3.45 5499 2.00 5919 3.0 x 150 mm 2.70 5977 1.40 6023 Table 2.
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[ CARE AND USE MANUAL ] a. Cleaning b. Effects of Additives Use the cleaning routine that matches the properties of the samples and/or what you believe is contaminating the column (see Table 4). Placing the column on an ACQUITY UPLC® System (or HPLC system) will enable the use of a wide range of solvents and water to improve cleaning. Flush columns with 20 column volumes of solvent. Increasing column temperature increases cleaning efficiency.
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[ CARE AND USE MANUAL ] V I. INT RODUC ING ECO RD INT E L LIG ENT C HI P T EC HNOLOGY c. Manufacturing Information a. Introduction The eCord Intelligent Chip will provide the history of a column’s performance throughout its lifetime. The eCord is permanently attached to the column to assure that the column’s performance history is maintained in the event that the column is moved from one instrument to another. Figure 3. eCord Intelligent Chip.
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[ CARE AND USE MANUAL ] d. Column Use Information V II. ACQUIT Y U P C 2 COLUMN FAMILY The eCord Chip provides the customer with column use data, column dimensions and serial number. The overall column usage information includes the total number of samples, total number of injections, total sample sets, date of first injection, date of last injection, maximum pressure and temperature.