Instruction Manual

[ CARE AND USE MANUAL ]
[ CARE AND USE MANUAL ]
4
2. If the column has been stored in 100% acetonitrile, condition
the column with acetonitrile/water (60%/40%) at 1 mL/min for
5 minutes before equilibrating in Eluent A.
3. After equilibrating the column, run a blank gradient.
IV. CARE AND MAINTENANCE
a. Storing the Column
For periods of three days or less, store the column in acetonitrile/water
(60%/40%). If the column will not be used for more than 72 hours,
store it in 100% acetonitrile.
Do not allow the column to dry out or to freeze.
Symptom Conditions Corrective action
Excess pressure
buildup
In-line precolumn filter plugged. Check for
injector or pump seal shedding.
Replace filter.
Column frit plugged Replace frit.
Clogged tubing Unclog or replace tubing.
Failing injector Repair the injector.
Column contaminated
Flush column with 100% acetonitrile at 0.5 ml/min for 30 minutes.
Loss of resolution
broad peaks,
low plate counts
Insufficient equilibration Continue equilibration.
Column contaminated Flush column with 100% acetonitrile at 0.5 ml/min for 30 minutes.
Incorrect diameter tubing Install 0.005 inch PEEK
™
tubing.
Table 2. Column Problems and Solutions