Instruction Manual

ManualsBrandsWaters ManualsWater equipmentAccQ-Tag Amino Acid Analysis Column

[ CARE AND USE MANUAL ]

I. INTRODUCTION

This manual describes procedures for using the Waters AccQ•Tag

™

Amino Acid Analysis column for the separation of hydrolysate amino

acids derivatized according to the AccQ•Tag

™

Method.

The Waters AccQ•Tag

™

Amino Acid Analysis column is manufactured

in Waters.

ISO 9002-certified facilities under strict guidelines to assure the high-

est quality products. Please take a few moments to read this manual

carefully. Follow its recommendations to ensure reproducible chroma-

tography and to maximize column lifetime.

a. Waters AccQ•Tag

™

Column Description

The AccQ•Tag

™

column separates the hydrolysate amino acid deriva-

tives produced in the reaction with Waters AccQ•Fluor

™

reagent. The

AccQ•Tag

™

column is a high-efficiency 4 μm Nova-Pak

™

column

specifically certified for use with the AccQ•Tag

™

Method.

When you use the AccQ•Tag

™

column as part of a Waters AccQ•Tag

™

system, sub-picomole detection limits can be achieved.

b. Specifications

CONTENTS

I. INTRODUCTION

a. Waters AccQ•Tag

™

Column Description

b. Specifications

II. INSTALLING THE COLUMN

a. Preparing the System

b. Installing the Columns

III. USING THE COLUMN

a. Chromatography Guidelines

b. Efficiency Testing

c. Equilibrating the Column for the AccQ•Tag

™

Method

IV. CARE AND MAINTENANCE

a. Storing the Column

APPENDIX A. SPARE PARTS

APPENDIX B. WARRANTY/SERVICE INFORMATION

ACCQ•TAG

™

AMINO ACID ANALYSIS COLUMN

Column AccQ•Tag

™

Amino Acid Analysis Column

Dimensions 3.9 mm x 150 mm

Particle Size 4 μm

Packing Material Silica base bonded with C

Shipped in Acetonitrile/water (50%/50%)

Summary of content (6 pages)

PAGE 1
[ CARE AND USE MANUAL ] ACCQ•TAG ™ AMINO ACID ANALYSIS COLUMN I. INT RODUC T ION CONTENTS ™ This manual describes procedures for using the Waters AccQ•Tag Amino Acid Analysis column for the separation of hydrolysate amino acids derivatized according to the AccQ•Tag™ Method. I. INTRODUCTION a. Waters AccQ•Tag™ Column Description b. Specifications ™ The Waters AccQ•Tag Amino Acid Analysis column is manufactured in Waters. II. INSTALLING THE COLUMN a.
PAGE 2
[ CARE AND USE MANUAL ] II. INSTALLING THE COLUMN To prepare a new stainless steel tubing-ferrule connection: a. Preparing the System 1. Use a file with a cutting edge or a tube cutter to scribe the circumference of the steel tubing at the desired break point. This procedure requires a zero dead-volume union. Before installing 2. Grasp the tubing on both sides of the scribe mark with cloth- the column in the flow path: covered pliers (to prevent marring the tube surface), and gently 1.
PAGE 3
[ CARE AND USE MANUAL ] 2. Inject 10 pl test standard and run the analysis for 10 minutes. • Protect the column from rapid changes in pressure, which can result from rapid changes in eluent composition or flow rate. 3. Record results, instrument settings, and instrument configuration ® for future comparison. • Use water that has been purified with a Millipore Water System capable of delivering 18 megohm water. De-ionized water may contain organic compounds that alter column selectivity. Table 1.
PAGE 4
[ CARE AND USE MANUAL ] 2. If the column has been stored in 100% acetonitrile, condition IV. CARE AND MAINTENANCE the column with acetonitrile/water (60%/40%) at 1 mL/min for a. Storing the Column 5 minutes before equilibrating in Eluent A. For periods of three days or less, store the column in acetonitrile/water 3. After equilibrating the column, run a blank gradient. (60%/40%). If the column will not be used for more than 72 hours, store it in 100% acetonitrile.
PAGE 5
[ CARE AND USE MANUAL ] APPENDIX A. SPARE PARTS To order the parts below, contact the nearest subsidiary (see back cover). Table A-1 Spare Parts and Accessories Quantity Part Number AccQ•Fluor Reagent Kit — WAT052880 AccQ•Tag™ Chemistry Package — WAT052875 — WAT052885 ™ ™ AccQ•Tag Column 3.
PAGE 6
[ CARE AND USE MANUAL ] Sales Offices: Austria and European Export (Central South Eastern Europe, CIS and Middle East) 43 1 877 18 07 India and India Subcontinent 91 80 2837 1900 Ireland 353 1 448 1500 Italy 39 02 27 421 1 Australia 61 2 9933 1777 Japan 81 3 3471 7191 Belgium 32 2 726 1000 Korea 82 2 820 2700 Brazil 55 11 5094 3788 Mexico 52 55 5524 7636 Canada 1 800 252 4752 x2205 The Netherlands 31 76 508 7200 China 86 10 8586 8899 Norway 47 6 384 60 50 CIS/Russia +7 495 3367000 Poland 48 22