Manual

[ Care and Use ManUal ]
AccQ•Fluor Reagent Kit 4
XI. sPecIFIcatIons
Waters manufactures the AccQ•Fluor Reagent Kit to exacting analyti-
cal specifications to minimize amino acid background. A 10 μL injec-
tion of a property prepared derivatizatlon blank (20 μL reconstituted
AccQ•Fluor Reagent plus 80 μL. AccQ•Fluor Borate Buffer) contains
less than 2 pmol of any of the hydrolysate amino acids (less than 4
pmol Cys).
Ensure all glassware is clean. For high sensitivity analyses, pyrolyze
all sample tubes at 500 °C for at least 4 hours.
XII. technIcal lIterature
Cohen, S.A. and D.P. Michaud, (1993) “Synthesis of a Fluorescent
Derivatizing Reagent, 6-Aminoquinolyl-N- Hydroxysuccinimidyl Car-
bamate, and its Application for the Analysis of Hydrolysale Amino
Acids via High Performance Liquid Chromatography,” Anal. Biochem.
211, pp 279.287.
Cohen, S.A., De Antonis, K., and D.P. Michaud, (1993) “Compositional
Protein Analysis Using 6-Aminoquinolyl-N-Hydroxysuccinimidyl
Carbamate, a Novel Derivatization Reagent,” Techniques In Protein
Chemistry IV, pp 289.298, (R.H. Angeletil, Ed.) Academic Press, San
Diego, CA.
XIII. orderIng InForMatIon
Item Part Number
AccQ•Fluor Reagent Kit WAT052880
AccQ•Tag Chemistry Package WAT052875
AccQ•Tag Column, 3.9 x 150 mm WAT052885
AccQ•Tag Eluent A Concentrate, 950 ml WAT052890
AccQ•Tag EIuent B, 950 ml WAT052895
Amino Acid Hydrolysale Standard WAT 08 8122
Aulosampler Vials, 4 ml, Glass, 48/pkg WAT072710
Caps for 4 ml Aulosampler Vials, 144/pkg W AT 0 79711
Septa for 4 mL Aulosampler Vials, 1440/pkg WAT073005
150 μL Low Volume Inserts, 144/pkg WAT072704
Springs for Low Volume Inserts, 144/pkg WAT072708
Sample tubes, 6 x 50 mm, 72/pkg WAT00757I