Instruction Manual

Table Of Contents

ANNEX B: DEPArray recoveries preparation for downstream genetic analysis

8. If the samples contain two drops, refer to the following table:

If samples come

from...

And the contained

cells are...

Then remove from the

meniscus...

DEPArray V2 fixed -

live 20 µl of cell medium

DEPArray NxT fixed -

live 16 µl of cell medium

9. If the samples contain six drops, refer to the following table:

If samples come

from...

And the contained

cells are...

Then remove from the

meniscus...

DEPArray V2 fixed 70 µl of DABUF

live 95 µl of cell culture medium

DEPArray NxT fixed 50 µl of DABUF

live 83 µl of cell culture medium

10. Add 100 µl of 1X PBS in each tube with a pipette and close the tubes.

11. Put the pipette tip against the wall at the top of the tube to dispense the liquid.

12. Insert the centrifuge racks in the swing bucket centrifuge.

13. Centrifuge the sample as follows:

If the sample

contains...

Then centrifuge at...

live cells 300 g x 15 min

fixed cells 1,000 g x 10 min

Result: the sample is now ready for the volume reduction to ~2 µl (see "Volume reduction to 3 or 2 µl" on page30).